IR@PKUHSC  > 北京大学第三临床医学院  > 骨科
学科主题临床医学
Simvastatin induces estrogen receptor-alpha (ER-alpha) in murine bone marrow stromal cells
Song, Chunli1; Wang, Jingying1; Song, Quansheng1; Li, Xu1; Chen, Zhongqiang1; Ma, Qingjun1; Liu, Zhongjun1; Jia, Hongti1,2; Dang, Gengting1
关键词simvastatin bone marrow stromal cells (BMSCs) osteoblast cell differentiation estrogen receptor alpha (ER-alpha) osteoporosis
刊名JOURNAL OF BONE AND MINERAL METABOLISM
2008-05-01
DOI10.1007/s00774-007-0820-6
26期:3页:213-217
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Medicine, Research & Experimental
研究领域[WOS]Endocrinology & Metabolism ; Research & Experimental Medicine
关键词[WOS]HYPERCHOLESTEROLEMIC POSTMENOPAUSAL WOMEN ; HORMONE REPLACEMENT THERAPY ; COA REDUCTASE INHIBITORS ; MINERAL DENSITY ; OSTEOBLASTIC DIFFERENTIATION ; GENE-EXPRESSION ; KOREAN WOMEN ; STATINS ; OSTEOPOROSIS ; FRACTURE
英文摘要

Simvastatin has been shown to stimulate osteogenesis both in vitro and in vivo. However, the mechanism by which simvastatin exerts its effects is still unclear. We previously reported that simvastatin promotes bone morphogenetic protein 2 (BMP-2) expression, induces osteoblastic differentiation, and inhibits adipocytic differentiation in mouse bone marrow stromal cells (BMSCs), and that this occurs, at least in part, via a BMP-2-dependent pathway. The aim of this study was to investigate further the mechanisms by which simvastatin stimulates osteogenesis in mouse BMSCs. To determine whether simvastatin-mediated osteogenesis was dependent on BMP-2, mouse BMSCs were treated with nonimmune normal mouse IgG or BMP-2 neutralizing antibodies combined with different concentrations of simvastatin. Surprisingly, the stimulatory effect of simvastatin on alkaline phosphatase (ALP) activity was not completely blocked by neutralizing BMP-2 monoclonal antibody treatment. Interestingly, we found that estrogen receptor-alpha (ER-alpha) protein levels increased after mouse BMSCs were treated with simvastatin for 72 h in a concentration-dependent manner. Moreover, the stimulatory effect of simvastatin on ALP activity in BMSCs was blocked by the estrogen receptor agonist ICI 182,780, and cotreatment with 17-beta-estradiol and simvastatin increased ALP activities by two-to threefold in the BMSCs compared with treatment with simvastatin alone. These results suggest that simvastatin-induced in vitro osteogenesis in mouse BMSCs is mediated, at least in part, by induction of ER-alpha and not by BMP-2 alone. These results provide new insight into the mechanisms of simvastatin-induced bone formation in BMSCs.

语种英语
WOS记录号WOS:000255746200002
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64086
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Peking Univ Hosp 3, Dept Orthoped, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Song, Chunli,Wang, Jingying,Song, Quansheng,et al. Simvastatin induces estrogen receptor-alpha (ER-alpha) in murine bone marrow stromal cells[J]. JOURNAL OF BONE AND MINERAL METABOLISM,2008,26(3):213-217.
APA Song, Chunli.,Wang, Jingying.,Song, Quansheng.,Li, Xu.,Chen, Zhongqiang.,...&Dang, Gengting.(2008).Simvastatin induces estrogen receptor-alpha (ER-alpha) in murine bone marrow stromal cells.JOURNAL OF BONE AND MINERAL METABOLISM,26(3),213-217.
MLA Song, Chunli,et al."Simvastatin induces estrogen receptor-alpha (ER-alpha) in murine bone marrow stromal cells".JOURNAL OF BONE AND MINERAL METABOLISM 26.3(2008):213-217.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Song, Chunli]的文章
[Wang, Jingying]的文章
[Song, Quansheng]的文章
百度学术
百度学术中相似的文章
[Song, Chunli]的文章
[Wang, Jingying]的文章
[Song, Quansheng]的文章
必应学术
必应学术中相似的文章
[Song, Chunli]的文章
[Wang, Jingying]的文章
[Song, Quansheng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。