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Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress
Zhao, Yuebin2; Ye, Lihua1; Liu, Huixue1; Xia, Qing1; Zhang, Yue1; Yang, Xiaoda2; Wang, Kui1
关键词Vanadium Mitochondria Permeability transition pore
刊名JOURNAL OF INORGANIC BIOCHEMISTRY
2010-04-01
DOI10.1016/j.jinorgbio.2009.11.007
104期:4页:371-378
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]RAT-LIVER MITOCHONDRIA ; CYTOCHROME-C-RELEASE ; DIABETIC-RATS ; TOXICITY ; COMPLEXES ; PHOSPHORYLATION ; VANADATE ; CELLS ; CYTOTOXICITY ; MECHANISMS
英文摘要

Vanadium compounds have been regarded as promising in therapeutic treatment of diabetes and in cancer prevention. In the present work, we studied the effects of vanadium compounds on mitochondria to investigate the mechanisms of toxicity. Mitochondria were isolated from rat liver and incubated with a variety of vanadium compounds, i.e. VOSO(4), NaVO(3), and vanadyl complexes with organic ligands. Our studies indicated that VO(2+), VO(3)(-), VO(acac)(2) and VOcit (1-100 mu M) could induce mitochondrial swelling in a concentration dependent manner and disrupt mitochondrial membrane potential (Delta psi(m)) in a time dependent manner, which is quite different from the rapid Delta psi(m) collapse caused by Ca(2+) or CCCP (carbonyl cyanide m-chlorophenylhydrazone, a mitochondrial uncoupling reagent). Release of cytochrome c (Cyt c) was observed and could be inhibited by cyclosporin A (CsA), an inhibitor of the mitochondrial permeability transition pore (PTP). Interestingly, VOdipic caused release of Cyt c without mitochondrial swelling and Delta psi(m) disruption, an action previously only observed on the Bax protein, suggesting a potentially role of VOdipic in regulating PTP opening. In addition, all the vanadium compounds tested stimulated mitochondrial production of reactive oxygen species (ROS). Antioxidants, i.e. vitamin C and E, significantly delayed the Delta psi(m) disruption. Overall, our experimental evidence indicated vanadium compounds exhibited multiple actions on mitochondria. Vanadium compounds did induce oxidative stress on mitochondrial and thus caused PTP opening, which led to collapse of Delta psi(m) and Cyt c release as the initiation of cell apoptosis. (C) 2009 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000274921200002
引用统计
被引频次:79[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64088
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, State Key Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China
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GB/T 7714
Zhao, Yuebin,Ye, Lihua,Liu, Huixue,et al. Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2010,104(4):371-378.
APA Zhao, Yuebin.,Ye, Lihua.,Liu, Huixue.,Xia, Qing.,Zhang, Yue.,...&Wang, Kui.(2010).Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress.JOURNAL OF INORGANIC BIOCHEMISTRY,104(4),371-378.
MLA Zhao, Yuebin,et al."Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress".JOURNAL OF INORGANIC BIOCHEMISTRY 104.4(2010):371-378.
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