北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 化学生物学系  > 期刊论文
学科主题: 药学
题名:
Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress
作者: Zhao, Yuebin2; Ye, Lihua1; Liu, Huixue1; Xia, Qing1; Zhang, Yue1; Yang, Xiaoda2; Wang, Kui1
关键词: Vanadium ; Mitochondria ; Permeability transition pore
刊名: JOURNAL OF INORGANIC BIOCHEMISTRY
发表日期: 2010-04-01
DOI: 10.1016/j.jinorgbio.2009.11.007
卷: 104, 期:4, 页:371-378
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: RAT-LIVER MITOCHONDRIA ; CYTOCHROME-C-RELEASE ; DIABETIC-RATS ; TOXICITY ; COMPLEXES ; PHOSPHORYLATION ; VANADATE ; CELLS ; CYTOTOXICITY ; MECHANISMS
英文摘要:

Vanadium compounds have been regarded as promising in therapeutic treatment of diabetes and in cancer prevention. In the present work, we studied the effects of vanadium compounds on mitochondria to investigate the mechanisms of toxicity. Mitochondria were isolated from rat liver and incubated with a variety of vanadium compounds, i.e. VOSO(4), NaVO(3), and vanadyl complexes with organic ligands. Our studies indicated that VO(2+), VO(3)(-), VO(acac)(2) and VOcit (1-100 mu M) could induce mitochondrial swelling in a concentration dependent manner and disrupt mitochondrial membrane potential (Delta psi(m)) in a time dependent manner, which is quite different from the rapid Delta psi(m) collapse caused by Ca(2+) or CCCP (carbonyl cyanide m-chlorophenylhydrazone, a mitochondrial uncoupling reagent). Release of cytochrome c (Cyt c) was observed and could be inhibited by cyclosporin A (CsA), an inhibitor of the mitochondrial permeability transition pore (PTP). Interestingly, VOdipic caused release of Cyt c without mitochondrial swelling and Delta psi(m) disruption, an action previously only observed on the Bax protein, suggesting a potentially role of VOdipic in regulating PTP opening. In addition, all the vanadium compounds tested stimulated mitochondrial production of reactive oxygen species (ROS). Antioxidants, i.e. vitamin C and E, significantly delayed the Delta psi(m) disruption. Overall, our experimental evidence indicated vanadium compounds exhibited multiple actions on mitochondria. Vanadium compounds did induce oxidative stress on mitochondrial and thus caused PTP opening, which led to collapse of Delta psi(m) and Cyt c release as the initiation of cell apoptosis. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 20671008 ; 20971008
项目资助者: NSFC
WOS记录号: WOS:000274921200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64088
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, State Key Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Zhao, Yuebin,Ye, Lihua,Liu, Huixue,et al. Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2010,104(4):371-378.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Yuebin]'s Articles
[Ye, Lihua]'s Articles
[Liu, Huixue]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Yuebin]‘s Articles
[Ye, Lihua]‘s Articles
[Liu, Huixue]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace