IR@PKUHSC  > 北京大学药学院
学科主题药学
Honokiol protects brain against ischemia-reperfusion injury in rats through disrupting PSD95-nNOS interaction
Hu, Zhenyu1; Bian, Xiling1; Liu, Xiaoyan1; Zhu, Yuanjun1; Zhang, Xiaoyi2; Chen, Shizhong3; Wang, Kewei1; Wang, Yinye1
关键词Honokiol Cerebral ischemia-reperfusion Therapeutic time window PSD95-nNOS NMDA
刊名BRAIN RESEARCH
2013-01-23
DOI10.1016/j.brainres.2012.11.004
1491页:204-212
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]CEREBRAL-ARTERY OCCLUSION ; NMDA RECEPTOR ; IN-VIVO ; DAMAGE ; STROKE ; APOPTOSIS ; MAGNOLOL ; NNOS ; MICE ; ACTIVATION
英文摘要

Honokiol, a major bioactive constituent of the bark of Magnolia officinalis has been confirmed to have the neuroprotective effect on ischemic stroke in rats. This study was designed to observe the therapeutic time window of honokiol microemulsion on cerebral ischemia-reperfusion injury to support its potential for future clinical trials and further explore the underlying mechanisms. Honokiol microemulsion (50 mu g/kg, i.v. at 0, 1 or 3 h after reperfusion) significantly reduced neurological deficit, infarct volume and brain water content in rats subjected to cerebral ischemia-reperfusion, and honokiol (0.1-10 mu M) significantly attenuated oxygen-glucose deprivation- or glutamate-induced injury of fetal rat cortical neurons. In co-immunoprecipitation and western blot test, honokiol decreased the intensity of nNOS related to PSD95 but failed to affect that of PSD95 related to NR2B in NR2B-PSD95-nNOS complex, and it also inhibited the translocation of nNOS from cytosol to membrane without affecting total nNOS expression, and then markedly decreased NO production in cortical neurons. Besides, the results of whole-cell patch-clamp recordings showed that honokiol reversibly inhibited the NMDA current by about 64%. In conclusion, honokiol has a therapeutic window of at least 5 h after the onset of cerebral ischemia or 3 h after reperfusion in rats, which may be in part ascribed to the disruption of the PSD95-nNOS interaction leading to the inhibition of neurotoxic NO production. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000314321600022
项目编号2009zx09102-146 ; 81000552
资助机构National Science and Technology Major Project ; Natural Science Foundation of China
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64117
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Capital Med Univ, Beijing Area Major Lab Peptide & Small Mol Drugs, Coll Pharmaceut Sci & Chem Biol, Beijing 100069, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Hu, Zhenyu,Bian, Xiling,Liu, Xiaoyan,et al. Honokiol protects brain against ischemia-reperfusion injury in rats through disrupting PSD95-nNOS interaction[J]. BRAIN RESEARCH,2013,1491:204-212.
APA Hu, Zhenyu.,Bian, Xiling.,Liu, Xiaoyan.,Zhu, Yuanjun.,Zhang, Xiaoyi.,...&Wang, Yinye.(2013).Honokiol protects brain against ischemia-reperfusion injury in rats through disrupting PSD95-nNOS interaction.BRAIN RESEARCH,1491,204-212.
MLA Hu, Zhenyu,et al."Honokiol protects brain against ischemia-reperfusion injury in rats through disrupting PSD95-nNOS interaction".BRAIN RESEARCH 1491(2013):204-212.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hu, Zhenyu]的文章
[Bian, Xiling]的文章
[Liu, Xiaoyan]的文章
百度学术
百度学术中相似的文章
[Hu, Zhenyu]的文章
[Bian, Xiling]的文章
[Liu, Xiaoyan]的文章
必应学术
必应学术中相似的文章
[Hu, Zhenyu]的文章
[Bian, Xiling]的文章
[Liu, Xiaoyan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。