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学科主题: 基础医学
题名:
Enhanced osteogenic differentiation of mesenchymal stem cells on poly(L-lactide) nanofibrous scaffolds containing carbon nanomaterials
作者: Duan, Shun1; Yang, Xiaoping1,2; Mei, Fang3; Tang, Yan3; Li, Xiaoli4; Shi, Yuzhou1; Mao, Jifu1; Zhang, Hongquan3; Cai, Qing1,2
关键词: osteogenic differentiation ; nanofibrous scaffold ; carbon nanotube ; graphene
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2015-04-01
DOI: 10.1002/jbm.a.35283
卷: 103, 期:4, 页:1424-1435
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: PHASE-SEPARATION ; OSTEOBLAST DIFFERENTIATION ; STROMAL CELLS ; IN-VITRO ; NANOTUBES ; GRAPHENE ; MATRIX ; PROLIFERATION ; COMPOSITES ; ATTACHMENT
英文摘要:

Carbon nanomaterials (CNMs), such as carbon nanotube (CNT) and graphene, are highlighted in bone regeneration because of their osteoinductive properties. Their combinations with nanofibrous polymeric scaffolds, which mimic the morphology of natural extracellular matrix of bone, arouse keen interest in bone tissue engineering. To this end, CNM were incorporated into nanofibrous poly(l-lactic acid) scaffolds by thermal-induced phase separation. The CNM-containing composite nanofibrous scaffolds were biologically evaluated by both in vitro co-culture of bone mesenchymal stem cells (BMSCs) and in vivo implantation. The nanofibrous structure itself demonstrated significant enhancement in cell adhesion, proliferation and oseogenic differentiation of BMSCs, and with the incorporation of CNM, the composite nanofibrous scaffolds further promoted osteogenic differentiation of BMSCs significantly. Between the two CNMs, graphene showed stronger effect in promoting osteogenic differentiation of BMSCs than CNT. The results of in vivo experiments revealed that the composite nanofibrous scaffolds had both good biocompatibility and strong ability in inducing osteogenesis. CNMs could remarkably enhance the expression of osteogenesis-related proteins as well as the formation of type I collagen. Similarly, the graphene-containing composite nanofibrous scaffolds demonstrated the strongest effect on inducing osteogenesis in vivo. These findings demonstrated that CNM-containing composite nanofibrous scaffolds were obviously more efficient in promoting osteogenesis than pure polymeric scaffolds. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1424-1435, 2015.

语种: 英语
所属项目编号: 2012CB933904 ; 2012BAI07B08 ; Z121100005212007 ; 51142004 ; NCET-11-0556
项目资助者: National Basic Research Program of China ; National Key Technology Research and Development Program of China ; Beijing Municipal Science &amp ; Technology Commission ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University
WOS记录号: WOS:000350395300014
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64130
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China
2.Beijing Univ Chem Technol, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
4.Tsinghua Univ, Res Inst, Key Lab Biomed Mat & Implants, Shenzhen 518057, Peoples R China

Recommended Citation:
Duan, Shun,Yang, Xiaoping,Mei, Fang,et al. Enhanced osteogenic differentiation of mesenchymal stem cells on poly(L-lactide) nanofibrous scaffolds containing carbon nanomaterials[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2015,103(4):1424-1435.
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