学科主题临床医学
Metabonomic analysis of liver tissue from BALB/c mice with d-galactosamine/lipopolysaccharide-induced acute hepatic failure
Feng, Bo1; Wu, Shengming2; Liu, Feng1; Gao, Yan1; Dong, Fangting2; Wei, Lai1
关键词Acute liver failure Metabonomics GC/MS Liver tissue
刊名BMC GASTROENTEROLOGY
2013-04-29
DOI10.1186/1471-230X-13-73
13
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]NMR-BASED METABONOMICS ; HEPATOTOXICITY ; RAT ; CIRRHOSIS ; OUTCOMES
英文摘要

Background: Compared with biofluids, target tissues and organs more directly reflect the pathophysiological state of a disease process. In this study, a D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced mouse model was constructed to investigate metabonomics of liver tissue and directly characterize metabolic changes in acute liver failure (ALF).

Methods: After pretreatment of liver tissue, gas chromatography coupled to time-of-flight mass spectrometry (GC/TOFMS) was used to separate and identify the liver metabolites. Partial least squares - discriminant analysis models were constructed to separate the ALF and control groups and to find those compounds whose liver levels differed significantly between the two groups.

Results: Distinct clustering was observed between the ALF and control mice. Fifty-eight endogenous metabolites were identified. Compared with the control mice, many metabolites, including sugars, amino acids, fatty acids, and organic acids, underwent significant changes in the ALF group, some of which differed from changes observed in plasma. Significant reduction of some important intermediate metabolites indicates that production of ketone bodies, the tricarboxylic acid and urea cycles, gluconeogenesis, glycolysis and pentose phosphate pathways are inhibited after GalN/LPS administration.

Conclusions: GC/TOFMS can be a powerful technique to perform metabonomic studies of liver tissue. GalN/LPS treatment can severely disturb substance metabolism in the liver, with different effects on metabolites compared with those observed in the plasma.

语种英语
WOS记录号WOS:000318506900001
项目编号2008ZX10002-012 ; 2008ZX10002-013 ; 7122191 ; RDB2009-16
资助机构National S&amp ; T Major Project for Infectious Diseases Control ; Beijing Natural Science Foundation ; Peking University People&prime ; s Hospital Research and Development Fund
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64141
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
2.Natl Ctr Biomed Anal, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Feng, Bo,Wu, Shengming,Liu, Feng,et al. Metabonomic analysis of liver tissue from BALB/c mice with d-galactosamine/lipopolysaccharide-induced acute hepatic failure[J]. BMC GASTROENTEROLOGY,2013,13.
APA Feng, Bo,Wu, Shengming,Liu, Feng,Gao, Yan,Dong, Fangting,&Wei, Lai.(2013).Metabonomic analysis of liver tissue from BALB/c mice with d-galactosamine/lipopolysaccharide-induced acute hepatic failure.BMC GASTROENTEROLOGY,13.
MLA Feng, Bo,et al."Metabonomic analysis of liver tissue from BALB/c mice with d-galactosamine/lipopolysaccharide-induced acute hepatic failure".BMC GASTROENTEROLOGY 13(2013).
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