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Mechanistic roles of leptin in osteogenic stimulation in thoracic ligament Flavum cells
Fan, Dongwei; Chen, Zhongqiang; Chen, Yupeng; Shang, Yongfeng
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2007-10-12
DOI10.1074/jbc.M611779200
282期:41页:29958-29966
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]POSTERIOR LONGITUDINAL LIGAMENT ; RECEPTOR-REGULATED TRANSCRIPTION ; BONE-FORMATION ; OSTEOBLAST DIFFERENTIATION ; ENDOMETRIAL CARCINOGENESIS ; MOLECULAR-MECHANISMS ; STAT3 ACTIVATION ; OSTEOCALCIN GENE ; SPINAL LIGAMENTS ; YELLOW LIGAMENT
英文摘要

Obesity is a risk factor for thoracic ossification of ligament flavum ( TOLF) that is characterized by ectopic bone formation in the spinal ligaments. Hyperleptinemia is a common feature of obese people, and leptin, an adipocyte- derived cytokine with proliferative and osteogenic effects in several cell types, is believed to be an important factor in the pathogenesis of TOLF. However, how leptin might stimulate cell osteogenic differentiation in TOLF is not totally understood. We reported here that leptin- induced osteogenic effect inTOLFcells is associated with activation of signaling molecules STAT3, JNK, and ERK1/ 2 but not p38. Blocking STAT3 phosphorylation with a selective inhibitor, AG490, significantly abolished leptin- induced osteogenic differentiation of TOLF cells, whereas blocking ERK1/ 2 and JNK phosphorylation with their selective inhibitors PD98059 and SP600125, respectively, had only marginal effects. In addition, we showed that STAT3 interacted with Runt- related transcription factor 2 ( Runx2) in the nucleus, and STAT3, Runx2, and steroid receptor coactivator steroid receptor coactivator- 1 were components of the transcription complex recruitedon Runx2 target gene promoters in response to leptin treatment. Our experiments identified STAT3, Runx2, and steroid receptor coactivator1 as critical molecules in mediating leptin- stimulated cell osteogenesis in TOLF.

语种英语
WOS记录号WOS:000249981200025
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64166
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ Third Hosp, Dept Orthoped, Beijing 100083, Peoples R China
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Fan, Dongwei,Chen, Zhongqiang,Chen, Yupeng,et al. Mechanistic roles of leptin in osteogenic stimulation in thoracic ligament Flavum cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2007,282(41):29958-29966.
APA Fan, Dongwei,Chen, Zhongqiang,Chen, Yupeng,&Shang, Yongfeng.(2007).Mechanistic roles of leptin in osteogenic stimulation in thoracic ligament Flavum cells.JOURNAL OF BIOLOGICAL CHEMISTRY,282(41),29958-29966.
MLA Fan, Dongwei,et al."Mechanistic roles of leptin in osteogenic stimulation in thoracic ligament Flavum cells".JOURNAL OF BIOLOGICAL CHEMISTRY 282.41(2007):29958-29966.
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