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学科主题: 临床医学
题名:
Mechanistic roles of leptin in osteogenic stimulation in thoracic ligament Flavum cells
作者: Fan, Dongwei; Chen, Zhongqiang; Chen, Yupeng; Shang, Yongfeng
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2007-10-12
DOI: 10.1074/jbc.M611779200
卷: 282, 期:41, 页:29958-29966
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: POSTERIOR LONGITUDINAL LIGAMENT ; RECEPTOR-REGULATED TRANSCRIPTION ; BONE-FORMATION ; OSTEOBLAST DIFFERENTIATION ; ENDOMETRIAL CARCINOGENESIS ; MOLECULAR-MECHANISMS ; STAT3 ACTIVATION ; OSTEOCALCIN GENE ; SPINAL LIGAMENTS ; YELLOW LIGAMENT
英文摘要:

Obesity is a risk factor for thoracic ossification of ligament flavum ( TOLF) that is characterized by ectopic bone formation in the spinal ligaments. Hyperleptinemia is a common feature of obese people, and leptin, an adipocyte- derived cytokine with proliferative and osteogenic effects in several cell types, is believed to be an important factor in the pathogenesis of TOLF. However, how leptin might stimulate cell osteogenic differentiation in TOLF is not totally understood. We reported here that leptin- induced osteogenic effect inTOLFcells is associated with activation of signaling molecules STAT3, JNK, and ERK1/ 2 but not p38. Blocking STAT3 phosphorylation with a selective inhibitor, AG490, significantly abolished leptin- induced osteogenic differentiation of TOLF cells, whereas blocking ERK1/ 2 and JNK phosphorylation with their selective inhibitors PD98059 and SP600125, respectively, had only marginal effects. In addition, we showed that STAT3 interacted with Runt- related transcription factor 2 ( Runx2) in the nucleus, and STAT3, Runx2, and steroid receptor coactivator steroid receptor coactivator- 1 were components of the transcription complex recruitedon Runx2 target gene promoters in response to leptin treatment. Our experiments identified STAT3, Runx2, and steroid receptor coactivator1 as critical molecules in mediating leptin- stimulated cell osteogenesis in TOLF.

语种: 英语
WOS记录号: WOS:000249981200025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64166
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ Third Hosp, Dept Orthoped, Beijing 100083, Peoples R China

Recommended Citation:
Fan, Dongwei,Chen, Zhongqiang,Chen, Yupeng,et al. Mechanistic roles of leptin in osteogenic stimulation in thoracic ligament Flavum cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2007,282(41):29958-29966.
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