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学科主题基础医学
Identification of Genes to Differentiate Closely Related Salmonella Lineages
Zou, Qing-Hua1; Li, Ren-Qing2; Wang, Ye-Jun3; Liu, Shu-Lin1,3,4
刊名PLOS ONE
2013-02-18
DOI10.1371/journal.pone.0055988
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GENOMIC CLEAVAGE MAP ; ENTERICA SEROVAR TYPHIMURIUM ; PATHOGENICITY ISLANDS ; TYPHI ; PARATYPHI ; EVOLUTION ; SEQUENCE ; REARRANGEMENTS ; RECOMBINATION ; CONVERGENCE
英文摘要

Background: Salmonella are important human and animal pathogens. Though highly related, the Salmonella lineages may be strictly adapted to different hosts or cause different diseases, from mild local illness like gastroenteritis to fatal systemic infections like typhoid. Therefore, rapid and accurate identification of Salmonella is essential for timely and correct diagnosis of Salmonella infections. The current identification methods such as 16S rRNA sequencing and multilocus sequence typing are expensive and time consuming. Additionally, these methods often do not have sufficient distinguishing resolution among the Salmonella lineages.

Methodologies/Principal Findings: We compared 27 completely sequenced Salmonella genomes to identify possible genomic features that could be used for differentiation of individual lineages. We concatenated 2372 core genes in each of the 27 genomes and constructed a neighbor-joining tree. On the tree, strains of each serotype were clustered tightly together and different serotypes were unambiguously separated with clear genetic distances, demonstrating systematic genomic divergence among the Salmonella lineages. We made detailed comparisons among the 27 genomes and identified distinct sets of genomic differences, including nucleotide variations and genomic islands (GIs), among the Salmonella lineages. Two core genes STM4261 and entF together could unambiguously distinguish all Salmonella lineages compared in this study. Additionally, strains of a lineage have a common set of GIs and closely related lineages have similar sets of GIs.

Conclusions: Salmonella lineages have accumulated distinct sets of mutations and laterally acquired DNA (e.g., GIs) in evolution. Two genes entF and STM4261 have diverged sufficiently among the Salmonella lineages to be used for their differentiation. Further investigation of the distinct sets of mutations and GIs will lead to novel insights into genomic evolution of Salmonella and greatly facilitate the elucidation of pathogeneses of Salmonella infections.

语种英语
WOS记录号WOS:000315159200017
项目编号31100134
资助机构National Natural Science Foundation of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64175
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing 100871, Peoples R China
2.Beijing Ctr Dis Control & Prevent, Inst Immunol, Beijing, Peoples R China
3.Harbin Med Univ, Genom Res Ctr, State Prov Key Labs Biomed Pharmaceut China, Harbin, Peoples R China
4.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
推荐引用方式
GB/T 7714
Zou, Qing-Hua,Li, Ren-Qing,Wang, Ye-Jun,et al. Identification of Genes to Differentiate Closely Related Salmonella Lineages[J]. PLOS ONE,2013,8(2).
APA Zou, Qing-Hua,Li, Ren-Qing,Wang, Ye-Jun,&Liu, Shu-Lin.(2013).Identification of Genes to Differentiate Closely Related Salmonella Lineages.PLOS ONE,8(2).
MLA Zou, Qing-Hua,et al."Identification of Genes to Differentiate Closely Related Salmonella Lineages".PLOS ONE 8.2(2013).
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