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HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules
Guan, Xiang-Yu1; Li, Hui-Fang1; Yang, Wen-Zhi1; Lin, Chang-Hu3; Sun, Chao2; Wang, Bao-Rong1; Guo, De-An1; Ye, Min1
关键词XLGB Quality control HPLC-DAD-MSn HPLC Epimedii Herba
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2011-07-15
DOI10.1016/j.jpba.2011.03.021
55期:5页:923-933
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]LIQUID-CHROMATOGRAPHY ; FLAVONOL GLYCOSIDES ; MASS-SPECTROMETRY ; PSORALEA-CORYLIFOLIA ; EPIMEDIUM-SAGITTATUM
英文摘要

In this study, a systematic method was established for the global quality control of Xian-ling-gu-bao capsules (XLGB), a popular six-herb Traditional Chinese Patent Medicine (TCPM) for the treatment of osteoporosis. Both qualitative and quantitative analyses were conducted. In qualitative analysis part, a fast and sensitive method based on high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-MSn) was established for rapid separation and sensitive identification. Samples were separated on a Waters Symmetry C-18 column (250 mm x 4.6 mm, 5 mu m) by gradient elution using acetonitrile (A) and water-formic acid (B; 0.03%, v/v) as mobile phase at a flow rate of 1.0 ml/min. Based on the mass spectra, UV spectra and retention time, 47 compounds were identified or tentatively characterized, including 27 flavonols (all from Epimedii Herba, the major component herb), 4 coumarins, 3 flavonones, 1 chalcone, 3 isoflavones, 1 coumestrol, 3 triterpenoid saponins, 1 iridoid, 3 steroidal saponins, and I phenolic acid. Among them, 18 compounds were confirmed by comparing with reference standards. In quantitative analysis part, 10 major compounds in 18 batches of XLGB were simultaneously determined by HPLC/UV detected at 270 nm. The method was validated with respect to intra- and inter-day precision, repeatability and stability, with RSD less than 1.0%, 1.5%, 2.9% and 1.8%, respectively. All the 10 analytes showed good linearity in wide linear ranges (r(2) = 0.9999), and their average recoveries varied between 97.8% and 104.9%. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000291129700009
项目编号985-2-119-121
资助机构Peking University Health Science Center
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64178
专题北京大学药学院
作者单位1.Guizhou Acad Sci, Inst Biol, Guiyang 550005, Peoples R China
2.Guizhou Acad Sci, Guiyang 550001, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Guan, Xiang-Yu,Li, Hui-Fang,Yang, Wen-Zhi,et al. HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,55(5):923-933.
APA Guan, Xiang-Yu.,Li, Hui-Fang.,Yang, Wen-Zhi.,Lin, Chang-Hu.,Sun, Chao.,...&Ye, Min.(2011).HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,55(5),923-933.
MLA Guan, Xiang-Yu,et al."HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 55.5(2011):923-933.
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