IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Potential of 3,4-dihydroxy-phenyl lactic acid for ameliorating ischemia-reperfusion-induced microvascular disturbance in rat mesentery
Han, Jing-Yan1,2,4; Horie, Yoshinori2; Fan, Jing-Yu4; Sun, Kai4; Guo, Jun2,4; Miura, Soichiro3; Hibi, Toshifumi2
关键词leukocyte adhesion mast cell degranulation oxidative stress Salvia miltiorrhiza
刊名AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
2009
DOI10.1152/ajpgi.90284.2008
296期:1页:G36-G44
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Physiology
研究领域[WOS]Gastroenterology & Hepatology ; Physiology
关键词[WOS]INDUCED LEUKOCYTE ADHESION ; SALVIA-MILTIORRHIZA ; MICROCIRCULATORY DISTURBANCE ; INJURY ; ISCHEMIA/REPERFUSION ; EXTRACT ; NEUTROPHILS ; ROSIGLITAZONE ; DYSFUNCTION ; PROTECTION
英文摘要

Han J-Y, Horie Y, Fan J-Y, Sun K, Guo J, Miura S, Hibi T. Potential of 3,4-dihydroxy-phenyl lactic acid for ameliorating ischemia-reperfusion- induced microvascular disturbance in rat mesentery. Am J Physiol Gastrointest Liver Physiol 296: G36-G44, 2009. First published November 13, 2008; doi:10.1152/ajpgi.90284.2008.-This study intended to examine the effect of 3,4- dihydroxy-phenyl lactic acid (DLA), a major ingredient of Salvia miltiorrhiza, on ischemia-reperfusion (I/R)-induced rat mesenteric microcirculatory injury. DLA (5 mg.kg(-1).h(-1)), superoxide dismutase (SOD, 12,000 U.kg(-1).h(-1)), or catalase (CAT, 20 mg/kg) was continuously infused either starting from 10 min before the ischemia or 10 min after the initiation of reperfusion. The venule diameter, number of adherent leukocytes, FITC-albumin leakage, dihydrorhodamine 123 fluorescence, and mast cell degranulation were determined using an intravital microscope. The production of hydrogen peroxide (H2O2) and the expression of adhesion molecules CD11b/CD18 in neutrophils were evaluated by in vitro experiments. The results showed that pretreatment with DLA significantly reduced peroxide production in and leukocyte adhesion to venular wall, albumin leakage, and mast cell degranulation induced by I/R. The DLA posttreatment exerted an ameliorating effect on I/R-induced disorders as well, characterized by inhibiting further increase in peroxide production in venular wall and albumin leakage and diminishing the number of leukocytes that had adhered to the venular wall. In vitro experiments revealed that treatment with DLA significantly attenuated TNF-alpha plus fMLP-evoked production of H2O2 and the H2O2-elicited expression of CD11b/CD18 on neutrophils. SOD and CAT manifested similarly but with the exception that either SOD or CAT were unable to retrieve the adherent leukocytes if administrated after initiation of reperfusion and to depress the H2O2-induced expression of CD11b/CD18 on neutrophils. It is concluded that DLA protects from and ameliorates the I/R-induced microcirculatory disturbance by interfering with both peroxide production and adhesion molecule expression.

语种英语
WOS记录号WOS:000262063400005
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64185
Collection北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100083, Peoples R China
2.Keio Univ, Sch Med, Dept Internal Med, Tokyo, Japan
3.Natl Def Med Coll, Dept Internal Med, Saitama, Japan
4.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Han, Jing-Yan,Horie, Yoshinori,Fan, Jing-Yu,et al. Potential of 3,4-dihydroxy-phenyl lactic acid for ameliorating ischemia-reperfusion-induced microvascular disturbance in rat mesentery[J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,2009,296(1):G36-G44.
APA Han, Jing-Yan.,Horie, Yoshinori.,Fan, Jing-Yu.,Sun, Kai.,Guo, Jun.,...&Hibi, Toshifumi.(2009).Potential of 3,4-dihydroxy-phenyl lactic acid for ameliorating ischemia-reperfusion-induced microvascular disturbance in rat mesentery.AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,296(1),G36-G44.
MLA Han, Jing-Yan,et al."Potential of 3,4-dihydroxy-phenyl lactic acid for ameliorating ischemia-reperfusion-induced microvascular disturbance in rat mesentery".AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY 296.1(2009):G36-G44.
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