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学科主题: 口腔医学
题名:
YAP Regulates the Expression of Hoxa1 and Hoxc13 in Mouse and Human Oral and Skin Epithelial Tissues
作者: Liu, Ming1; Zhao, Shuangyun1,2; Lin, Qingjie1,3; Wang, Xiu-Ping1
刊名: MOLECULAR AND CELLULAR BIOLOGY
发表日期: 2015-04-01
DOI: 10.1128/MCB.00765-14
卷: 35, 期:8, 页:1449-1461
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: YES-ASSOCIATED PROTEIN ; HIPPO SIGNALING-PATHWAY ; NAIL ECTODERMAL DYSPLASIA ; TUMOR-SUPPRESSOR PATHWAY ; ACID RESPONSE ELEMENT ; EMBRYONIC STEM-CELLS ; ORGAN SIZE CONTROL ; GENE-EXPRESSION ; RETINOIC ACID ; CARDIOMYOCYTE PROLIFERATION
英文摘要:

Yes-associated protein (YAP) is a Hippo signaling transcriptional coactivator that plays pivotal roles in stem cell proliferation, organ size control, and tumor development. The downstream targets of YAP have been shown to be highly context dependent. In this study, we used the embryonic mouse tooth germ as a tool to search for the downstream targets of YAP in ectoderm-derived tissues. Yap deficiency in the dental epithelium resulted in a small tooth germ with reduced epithelial cell proliferation. We compared the gene expression profiles of embryonic day 14.5 (E14.5) Yap conditional knockout and YAP transgenic mouse tooth germs using transcriptome sequencing (RNA-Seq) and further confirmed the differentially expressed genes using real-time PCR and in situ hybridization. We found that YAP regulates the expression of Hoxa1 and Hoxc13 in oral and dental epithelial tissues as well as in the epidermis of skin during embryonic and adult stages. Sphere formation assay suggested that Hoxa1 and Hoxc13 are functionally involved in YAP-regulated epithelial progenitor cell proliferation, and chromatin immunoprecipitation (ChIP) assay implies that YAP may regulate Hoxa1 and Hoxc13 expression through TEAD transcription factors. These results provide mechanistic insights into abnormal YAP activities in mice and humans.

语种: 英语
项目资助者: HSDM
WOS记录号: WOS:000351741500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64191
Appears in Collections:北京大学口腔医学院_儿童口腔科_期刊论文

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作者单位: 1.Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 USA
2.Peking Univ, Sch Stomatol, Dept Pediat Dent, Beijing 100871, Peoples R China
3.Shandong Univ, Sch Stomatol, Dept Implantat, Jinan 250100, Peoples R China

Recommended Citation:
Liu, Ming,Zhao, Shuangyun,Lin, Qingjie,et al. YAP Regulates the Expression of Hoxa1 and Hoxc13 in Mouse and Human Oral and Skin Epithelial Tissues[J]. MOLECULAR AND CELLULAR BIOLOGY,2015,35(8):1449-1461.
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