IR@PKUHSC  > 北京大学口腔医学院  > 儿童口腔科
学科主题口腔医学
YAP Regulates the Expression of Hoxa1 and Hoxc13 in Mouse and Human Oral and Skin Epithelial Tissues
Liu, Ming1; Zhao, Shuangyun1,2; Lin, Qingjie1,3; Wang, Xiu-Ping1
刊名MOLECULAR AND CELLULAR BIOLOGY
2015-04-01
DOI10.1128/MCB.00765-14
35期:8页:1449-1461
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]YES-ASSOCIATED PROTEIN ; HIPPO SIGNALING-PATHWAY ; NAIL ECTODERMAL DYSPLASIA ; TUMOR-SUPPRESSOR PATHWAY ; ACID RESPONSE ELEMENT ; EMBRYONIC STEM-CELLS ; ORGAN SIZE CONTROL ; GENE-EXPRESSION ; RETINOIC ACID ; CARDIOMYOCYTE PROLIFERATION
英文摘要

Yes-associated protein (YAP) is a Hippo signaling transcriptional coactivator that plays pivotal roles in stem cell proliferation, organ size control, and tumor development. The downstream targets of YAP have been shown to be highly context dependent. In this study, we used the embryonic mouse tooth germ as a tool to search for the downstream targets of YAP in ectoderm-derived tissues. Yap deficiency in the dental epithelium resulted in a small tooth germ with reduced epithelial cell proliferation. We compared the gene expression profiles of embryonic day 14.5 (E14.5) Yap conditional knockout and YAP transgenic mouse tooth germs using transcriptome sequencing (RNA-Seq) and further confirmed the differentially expressed genes using real-time PCR and in situ hybridization. We found that YAP regulates the expression of Hoxa1 and Hoxc13 in oral and dental epithelial tissues as well as in the epidermis of skin during embryonic and adult stages. Sphere formation assay suggested that Hoxa1 and Hoxc13 are functionally involved in YAP-regulated epithelial progenitor cell proliferation, and chromatin immunoprecipitation (ChIP) assay implies that YAP may regulate Hoxa1 and Hoxc13 expression through TEAD transcription factors. These results provide mechanistic insights into abnormal YAP activities in mice and humans.

语种英语
WOS记录号WOS:000351741500011
资助机构HSDM
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64191
专题北京大学口腔医学院_儿童口腔科
作者单位1.Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 USA
2.Peking Univ, Sch Stomatol, Dept Pediat Dent, Beijing 100871, Peoples R China
3.Shandong Univ, Sch Stomatol, Dept Implantat, Jinan 250100, Peoples R China
推荐引用方式
GB/T 7714
Liu, Ming,Zhao, Shuangyun,Lin, Qingjie,et al. YAP Regulates the Expression of Hoxa1 and Hoxc13 in Mouse and Human Oral and Skin Epithelial Tissues[J]. MOLECULAR AND CELLULAR BIOLOGY,2015,35(8):1449-1461.
APA Liu, Ming,Zhao, Shuangyun,Lin, Qingjie,&Wang, Xiu-Ping.(2015).YAP Regulates the Expression of Hoxa1 and Hoxc13 in Mouse and Human Oral and Skin Epithelial Tissues.MOLECULAR AND CELLULAR BIOLOGY,35(8),1449-1461.
MLA Liu, Ming,et al."YAP Regulates the Expression of Hoxa1 and Hoxc13 in Mouse and Human Oral and Skin Epithelial Tissues".MOLECULAR AND CELLULAR BIOLOGY 35.8(2015):1449-1461.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Ming]的文章
[Zhao, Shuangyun]的文章
[Lin, Qingjie]的文章
百度学术
百度学术中相似的文章
[Liu, Ming]的文章
[Zhao, Shuangyun]的文章
[Lin, Qingjie]的文章
必应学术
必应学术中相似的文章
[Liu, Ming]的文章
[Zhao, Shuangyun]的文章
[Lin, Qingjie]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。