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学科主题临床医学
Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes on alpha(s1)-casein by alanine scanning analysis
Cong, Yanjun1,2; Yi, Hong1,2; Qing, Yuting1,2; Li, Linfeng3
关键词alpha(s1)-casein cow milk allergy epitope critical amino acid
刊名JOURNAL OF DAIRY SCIENCE
2013-11-01
DOI10.3168/jds.2013-6880
96期:11页:6870-6876
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Agriculture, Dairy & Animal Science ; Food Science & Technology
资助者National Natural Science Foundation of China (Beijing, China) ; Beijing Natural Science Foundation (China) ; Beijing New-Star Plan of Science and Technology, National Science and Technology Project in Rural Areas ; Beijing Municipal Commission of Education (China) ; National Natural Science Foundation of China (Beijing, China) ; Beijing Natural Science Foundation (China) ; Beijing New-Star Plan of Science and Technology, National Science and Technology Project in Rural Areas ; Beijing Municipal Commission of Education (China)
研究领域[WOS]Agriculture ; Food Science & Technology
关键词[WOS]BOVINE-MILK ALPHA-S1-CASEIN ; IGE-BINDING EPITOPES ; MUTATIONAL ANALYSIS ; NONATOPIC CHILDREN ; CELL EPITOPES ; ALLERGEN ; ANTIBODIES ; FOOD ; PERSISTENT ; OVALBUMIN
英文摘要

alpha(s1)-Casein represents one of the major allergens causing cow milk allergy. Few studies have clearly evaluated immunological relationships between IgE- and IgG-binding epitopes of alpha(s1)-casein. This study aimed to map IgE- and IgG-binding epitopes of alpha(s1)-casein by the serology method, and identify the critical amino acids of alpha(s1)-casein by alanine scanning analysis. Our initial data revealed IgE-binding epitopes located in the sequences of AA 126 to 140, AA 6 to 20, AA 171 to 185, and AA 11 to 25. The sequences at AA 21 to 35, AA 56 to 70, and AA 161 to 175 were recognized by IgG antibodies. The alanine scanning analysis showed that IgE- and IgG-binding epitopes share the same critical AA: arginine at position 22 and phenylalanine at position 23. Results obtained from this study will provide necessary information to alter the cDNA to encode a protein with reduced IgE- or IgG-binding capacity.

语种英语
所属项目编号31101236 ; 6132004 ; 2011AA100903 ; KM201110011003
资助者National Natural Science Foundation of China (Beijing, China) ; Beijing Natural Science Foundation (China) ; Beijing New-Star Plan of Science and Technology, National Science and Technology Project in Rural Areas ; Beijing Municipal Commission of Education (China) ; National Natural Science Foundation of China (Beijing, China) ; Beijing Natural Science Foundation (China) ; Beijing New-Star Plan of Science and Technology, National Science and Technology Project in Rural Areas ; Beijing Municipal Commission of Education (China)
WOS记录号WOS:000325664800010
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64204
Collection北京大学第三临床医学院_皮肤科
作者单位1.Beijing Technol & Business Univ, Engn Res Ctr Food Addit & Ingredients, Beijing Key Lab Food Flavor Chem, Beijing 100048, Peoples R China
2.Beijing Technol & Business Univ, Engn Res Ctr Food Addit & Ingredients, Beijing Higher Inst, Beijing 100048, Peoples R China
3.Peking Univ, Hosp 3, Dept Dermatol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Cong, Yanjun,Yi, Hong,Qing, Yuting,et al. Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes on alpha(s1)-casein by alanine scanning analysis[J]. JOURNAL OF DAIRY SCIENCE,2013,96(11):6870-6876.
APA Cong, Yanjun,Yi, Hong,Qing, Yuting,&Li, Linfeng.(2013).Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes on alpha(s1)-casein by alanine scanning analysis.JOURNAL OF DAIRY SCIENCE,96(11),6870-6876.
MLA Cong, Yanjun,et al."Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes on alpha(s1)-casein by alanine scanning analysis".JOURNAL OF DAIRY SCIENCE 96.11(2013):6870-6876.
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