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学科主题: 临床医学
题名:
Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes on alpha(s1)-casein by alanine scanning analysis
作者: Cong, Yanjun1,2; Yi, Hong1,2; Qing, Yuting1,2; Li, Linfeng3
关键词: alpha(s1)-casein ; cow milk allergy ; epitope ; critical amino acid
刊名: JOURNAL OF DAIRY SCIENCE
发表日期: 2013-11-01
DOI: 10.3168/jds.2013-6880
卷: 96, 期:11, 页:6870-6876
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Agriculture, Dairy & Animal Science ; Food Science & Technology
研究领域[WOS]: Agriculture ; Food Science & Technology
关键词[WOS]: BOVINE-MILK ALPHA-S1-CASEIN ; IGE-BINDING EPITOPES ; MUTATIONAL ANALYSIS ; NONATOPIC CHILDREN ; CELL EPITOPES ; ALLERGEN ; ANTIBODIES ; FOOD ; PERSISTENT ; OVALBUMIN
英文摘要:

alpha(s1)-Casein represents one of the major allergens causing cow milk allergy. Few studies have clearly evaluated immunological relationships between IgE- and IgG-binding epitopes of alpha(s1)-casein. This study aimed to map IgE- and IgG-binding epitopes of alpha(s1)-casein by the serology method, and identify the critical amino acids of alpha(s1)-casein by alanine scanning analysis. Our initial data revealed IgE-binding epitopes located in the sequences of AA 126 to 140, AA 6 to 20, AA 171 to 185, and AA 11 to 25. The sequences at AA 21 to 35, AA 56 to 70, and AA 161 to 175 were recognized by IgG antibodies. The alanine scanning analysis showed that IgE- and IgG-binding epitopes share the same critical AA: arginine at position 22 and phenylalanine at position 23. Results obtained from this study will provide necessary information to alter the cDNA to encode a protein with reduced IgE- or IgG-binding capacity.

语种: 英语
所属项目编号: 31101236 ; 6132004 ; 2011AA100903 ; KM201110011003
项目资助者: National Natural Science Foundation of China (Beijing, China) ; Beijing Natural Science Foundation (China) ; Beijing New-Star Plan of Science and Technology, National Science and Technology Project in Rural Areas ; Beijing Municipal Commission of Education (China)
WOS记录号: WOS:000325664800010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64204
Appears in Collections:北京大学第三临床医学院_皮肤科_期刊论文

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作者单位: 1.Beijing Technol & Business Univ, Engn Res Ctr Food Addit & Ingredients, Beijing Key Lab Food Flavor Chem, Beijing 100048, Peoples R China
2.Beijing Technol & Business Univ, Engn Res Ctr Food Addit & Ingredients, Beijing Higher Inst, Beijing 100048, Peoples R China
3.Peking Univ, Hosp 3, Dept Dermatol, Beijing 100083, Peoples R China

Recommended Citation:
Cong, Yanjun,Yi, Hong,Qing, Yuting,et al. Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes on alpha(s1)-casein by alanine scanning analysis[J]. JOURNAL OF DAIRY SCIENCE,2013,96(11):6870-6876.
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