北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室  > 期刊论文
学科主题: 临床医学
题名:
Evaluation of P38 MAPK Pathway as a Molecular Signature in Ulcerative Colitis
作者: Zhao, Xinmei1; Kang, Bin2,3; Lu, Chaolan1; Liu, Siqi2; Wang, Huanjing1; Yang, Xiaoming1; Chen, Ye1; Jiang, Bo1; Zhang, Jun2,3; Lu, Youyong2,3; Zhi, Fachao1
关键词: ulcerative colitis ; P38 MAPK pathway ; proteomics ; MAWBP ; molecular signature
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2011-05-01
DOI: 10.1021/pr100969w
卷: 10, 期:5, 页:2216-2225
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: INFLAMMATORY-BOWEL-DISEASE ; ACTIVATED PROTEIN-KINASE ; EXPRESSION ; CANCER ; CHINA ; GENE ; PHOSPHORYLATION ; HEALTH
英文摘要:

Early diagnosis and treatment of ulcerative colitis (UC) is clinically challenging. To overcome this problem, we explored the interrelated multiplex signaling pathway to identify molecular signatures in UC by using integrated strategy in proteomics. Intestinal mucosa of 12 UC cases and 12 normal controls underwent comparative proteomic analysis. A total of 26 unique differential proteins were identified, including 12 up-regulated and 14 down-regulated in UC group. A differential protein cluster, consisting of 11 proteins involved in p38 mitogen-activated protein kinase (MAPK) pathway, was deduced and validated by Western blot. Furthermore, three proteins elicited from the protein cluster, phosphorylated p38, MAWBP and galectin-3, as a molecular signature, were analyzed by immunohistochemistry on 118 UC and normal samples. Increased expression of P-p38 and down-regulated MAWBP and/or galectin-3 were detected in UC compared to normal samples (p < 0.001). This signature correlated with disease progression of UC (p < 0.01), and classified UC risk with high sensitivity (94.83 +/- 2.91%) and specificity (98.33 +/- 1.65%). In addition, P38 MAPK pathway modulated the expression of the protein clusters in macrophage cell line as evidenced by the alteration with specific inhibitor SB203580. These results indicate that molecular signature of P38 MAPK pathway might be a potential biomarker for evaluating UC risk.

语种: 英语
所属项目编号: 2009CB522204
项目资助者: 97-3 program
WOS记录号: WOS:000290234800008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64210
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.So Med Univ, Guangdong Prov Key Lab Gastroenterol, Dept Gastroenterol, Nanfang Hosp, Guangzhou 510515, Guangdong, Peoples R China
2.Chinese Acad Sci, Beijing Genom Inst, Beijing 101318, Peoples R China
3.Peking Univ Canc Hosp Inst, Mol Oncol Lab, Beijing 100142, Peoples R China

Recommended Citation:
Zhao, Xinmei,Kang, Bin,Lu, Chaolan,et al. Evaluation of P38 MAPK Pathway as a Molecular Signature in Ulcerative Colitis[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(5):2216-2225.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Xinmei]'s Articles
[Kang, Bin]'s Articles
[Lu, Chaolan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Xinmei]‘s Articles
[Kang, Bin]‘s Articles
[Lu, Chaolan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace