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学科主题临床医学
Evaluation of P38 MAPK Pathway as a Molecular Signature in Ulcerative Colitis
Zhao, Xinmei1; Kang, Bin2,3; Lu, Chaolan1; Liu, Siqi2; Wang, Huanjing1; Yang, Xiaoming1; Chen, Ye1; Jiang, Bo1; Zhang, Jun2,3; Lu, Youyong2,3; Zhi, Fachao1
关键词ulcerative colitis P38 MAPK pathway proteomics MAWBP molecular signature
刊名JOURNAL OF PROTEOME RESEARCH
2011-05-01
DOI10.1021/pr100969w
10期:5页:2216-2225
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]INFLAMMATORY-BOWEL-DISEASE ; ACTIVATED PROTEIN-KINASE ; EXPRESSION ; CANCER ; CHINA ; GENE ; PHOSPHORYLATION ; HEALTH
英文摘要

Early diagnosis and treatment of ulcerative colitis (UC) is clinically challenging. To overcome this problem, we explored the interrelated multiplex signaling pathway to identify molecular signatures in UC by using integrated strategy in proteomics. Intestinal mucosa of 12 UC cases and 12 normal controls underwent comparative proteomic analysis. A total of 26 unique differential proteins were identified, including 12 up-regulated and 14 down-regulated in UC group. A differential protein cluster, consisting of 11 proteins involved in p38 mitogen-activated protein kinase (MAPK) pathway, was deduced and validated by Western blot. Furthermore, three proteins elicited from the protein cluster, phosphorylated p38, MAWBP and galectin-3, as a molecular signature, were analyzed by immunohistochemistry on 118 UC and normal samples. Increased expression of P-p38 and down-regulated MAWBP and/or galectin-3 were detected in UC compared to normal samples (p < 0.001). This signature correlated with disease progression of UC (p < 0.01), and classified UC risk with high sensitivity (94.83 +/- 2.91%) and specificity (98.33 +/- 1.65%). In addition, P38 MAPK pathway modulated the expression of the protein clusters in macrophage cell line as evidenced by the alteration with specific inhibitor SB203580. These results indicate that molecular signature of P38 MAPK pathway might be a potential biomarker for evaluating UC risk.

语种英语
WOS记录号WOS:000290234800008
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64210
专题北京大学临床肿瘤学院_分子肿瘤学研究室
作者单位1.So Med Univ, Guangdong Prov Key Lab Gastroenterol, Dept Gastroenterol, Nanfang Hosp, Guangzhou 510515, Guangdong, Peoples R China
2.Chinese Acad Sci, Beijing Genom Inst, Beijing 101318, Peoples R China
3.Peking Univ Canc Hosp Inst, Mol Oncol Lab, Beijing 100142, Peoples R China
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Zhao, Xinmei,Kang, Bin,Lu, Chaolan,et al. Evaluation of P38 MAPK Pathway as a Molecular Signature in Ulcerative Colitis[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(5):2216-2225.
APA Zhao, Xinmei.,Kang, Bin.,Lu, Chaolan.,Liu, Siqi.,Wang, Huanjing.,...&Zhi, Fachao.(2011).Evaluation of P38 MAPK Pathway as a Molecular Signature in Ulcerative Colitis.JOURNAL OF PROTEOME RESEARCH,10(5),2216-2225.
MLA Zhao, Xinmei,et al."Evaluation of P38 MAPK Pathway as a Molecular Signature in Ulcerative Colitis".JOURNAL OF PROTEOME RESEARCH 10.5(2011):2216-2225.
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