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3-D microarrays biochip for DNA amplification in polydimethylsiloxane (PDMS) elastomer
Yu, XM; Zhang, DC; Li, T; Hao, L; Li, XH
关键词polymerase chain reaction (PCR) polydimethlysiloxane (PDMS) inductively coupled plasma (ICP)
刊名SENSORS AND ACTUATORS A-PHYSICAL
2003-11-15
DOI10.1016/S0924-4247(03)00370-4
108期:1-3页:103-107
收录类别ISTP ; SCI
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Engineering, Electrical & Electronic ; Instruments & Instrumentation
研究领域[WOS]Engineering ; Instruments & Instrumentation
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; PCR AMPLIFICATION
英文摘要

In this paper, a rapid and low-cost fabrication technique for microarrays biocbips is presented, and the fluorescence-based polymerase chain reactions (PCR) were performed in the chip for DNA amplification. The chip is consisted of 1064 column chambers. Each chamber has a volume of 25 nl, with the diameter of 460 mum and depth of 200 mum. The PCR chips were made of inexpensive silicone elastomer-polydimethylsiloxane (PDMS) and rapidly fabricated with micro-molding technique. The three-dimensional (3-D) PDMS replicas were molded directly from 3-D negative silicon master, which was fabricated by deep etching technique under the inductively coupled plasma (ICP) system. We demonstrated that the fluorescence-based PCR could be performed in the PDMS chip with nanoliter-volumes. (C) 2003 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000186955600018
引用统计
被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64219
专题北京大学第一临床医学院_血液内科
作者单位1.Peking Univ, Inst Microelect, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Yu, XM,Zhang, DC,Li, T,et al. 3-D microarrays biochip for DNA amplification in polydimethylsiloxane (PDMS) elastomer[J]. SENSORS AND ACTUATORS A-PHYSICAL,2003,108(1-3):103-107.
APA Yu, XM,Zhang, DC,Li, T,Hao, L,&Li, XH.(2003).3-D microarrays biochip for DNA amplification in polydimethylsiloxane (PDMS) elastomer.SENSORS AND ACTUATORS A-PHYSICAL,108(1-3),103-107.
MLA Yu, XM,et al."3-D microarrays biochip for DNA amplification in polydimethylsiloxane (PDMS) elastomer".SENSORS AND ACTUATORS A-PHYSICAL 108.1-3(2003):103-107.
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