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学科主题: 临床医学
题名:
3-D microarrays biochip for DNA amplification in polydimethylsiloxane (PDMS) elastomer
作者: Yu, XM; Zhang, DC; Li, T; Hao, L; Li, XH
关键词: polymerase chain reaction (PCR) ; polydimethlysiloxane (PDMS) ; inductively coupled plasma (ICP)
刊名: SENSORS AND ACTUATORS A-PHYSICAL
发表日期: 2003-11-15
DOI: 10.1016/S0924-4247(03)00370-4
卷: 108, 期:1-3, 页:103-107
收录类别: ISTP ; SCI
文章类型: Proceedings Paper
WOS标题词: Science & Technology
类目[WOS]: Engineering, Electrical & Electronic ; Instruments & Instrumentation
研究领域[WOS]: Engineering ; Instruments & Instrumentation
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ; PCR AMPLIFICATION
英文摘要:

In this paper, a rapid and low-cost fabrication technique for microarrays biocbips is presented, and the fluorescence-based polymerase chain reactions (PCR) were performed in the chip for DNA amplification. The chip is consisted of 1064 column chambers. Each chamber has a volume of 25 nl, with the diameter of 460 mum and depth of 200 mum. The PCR chips were made of inexpensive silicone elastomer-polydimethylsiloxane (PDMS) and rapidly fabricated with micro-molding technique. The three-dimensional (3-D) PDMS replicas were molded directly from 3-D negative silicon master, which was fabricated by deep etching technique under the inductively coupled plasma (ICP) system. We demonstrated that the fluorescence-based PCR could be performed in the PDMS chip with nanoliter-volumes. (C) 2003 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000186955600018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64219
Appears in Collections:北京大学第一临床医学院_血液内科_期刊论文

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作者单位: 1.Peking Univ, Inst Microelect, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China

Recommended Citation:
Yu, XM,Zhang, DC,Li, T,et al. 3-D microarrays biochip for DNA amplification in polydimethylsiloxane (PDMS) elastomer[J]. SENSORS AND ACTUATORS A-PHYSICAL,2003,108(1-3):103-107.
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