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学科主题: 基础医学
题名:
Insulin induces proliferation and cardiac differentiation of P19CL6 cells in a dose-dependent manner
作者: Li, Wen-Yan1; Song, Yang-Liu1; Xiong, Cheng-Juan1; Lu, Pei-Qi2; Xue, Li-Xiang3; Yao, Chun-Xia1; Wang, Wei-Ping3; Zhang, Shu-Feng4; Zhang, Shan-Feng1; Wei, Qing-Xia1; Zhang, Yan-Yan5; Zhao, Ji-Min1; Zang, Ming-Xi1
关键词: BCL-2-antagonist of cell death ; cell differentiation ; cell growth ; cyclin D1 ; insulin
刊名: DEVELOPMENT GROWTH & DIFFERENTIATION
发表日期: 2013-09-01
DOI: 10.1111/dgd.12075
卷: 55, 期:7, 页:676-686
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Developmental Biology
研究领域[WOS]: Cell Biology ; Developmental Biology
关键词[WOS]: PLURIPOTENT STEM-CELLS ; CYCLIN D1 ; CARDIOMYOCYTE DIFFERENTIATION ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; GLUCOSE-UPTAKE ; PATHWAY ; GATA-4 ; MYOGENESIS ; GROWTH
英文摘要:

Insulin is a peptide hormone produced by beta cells of the pancreas. The roles of insulin in energy metabolism have been well studied, with most of the attention focused on glucose utilization, but the roles of insulin in cell proliferation and differentiation remain unclear. In this study, we observed for the first time that 10 nmol/L insulin treatment induces cell proliferation and cardiac differentiation of P19CL6 cells, whereas 50 and 100 nmol/L insulin treatment induces P19CL6 cell apoptosis and blocks cardiac differentiation of P19CL6 cells. By using real-time polymerase chain reaction (PCR) and Western blotting analysis, we found that the mRNA levels of cyclin D1 and alpha myosin heavy chain (alpha-MHC) are induced upon 10 nmol/L insulin stimulation and inhibited upon 50/100 nmol/L insulin treatment, whereas the mRNA levels of BCL-2-antagonist of cell death (BAD) exists a reverse trend. The similar results were observed in P19CL6 cells expressing GATA-6 or peroxisome proliferator-activated receptor alpha (PPAR alpha). Our results identified the downstream targets of insulin, cyclin D1, BAD, alpha-MHC, and GATA-4, elucidate a novel molecular mechanism of insulin in promoting cell proliferation and differentiation.

语种: 英语
所属项目编号: 2007AA02Z122 ; 2012AA022501 ; 81141044 ; 81371895 ; 31071206 ; 082102310024 ; 104300510016
项目资助者: Ministry of Science and Technology of China (863 Program) ; National Natural Science Foundation of China ; International Cooperation and Exchange Foundation of Henan Scientific Committee ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, the State Education Ministry (SRF for ROCS, SEM) ; Ministry of Personnel ; Henan University Key Teacher Foundation
WOS记录号: WOS:000324434400003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64220
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Zhengzhou Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Zhengzhou 450001, Henan, Peoples R China
2.Zhengzhou Univ, Affiliated Hosp 2, Zhengzhou 450001, Henan, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
4.Zhengzhou Univ, Peoples Hosp Henan Prov, Zhengzhou 450001, Henan, Peoples R China
5.Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450001, Henan, Peoples R China

Recommended Citation:
Li, Wen-Yan,Song, Yang-Liu,Xiong, Cheng-Juan,et al. Insulin induces proliferation and cardiac differentiation of P19CL6 cells in a dose-dependent manner[J]. DEVELOPMENT GROWTH & DIFFERENTIATION,2013,55(7):676-686.
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