北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA)
作者: Xiao, Meng1,2; Guo, Li-Na1; Kong, Fanrong3; Wang, He1; Sorrell, Tania C.3; Li, Ruo-Yu4; Jiang, Wei5; Chen, Sharon C-A.3; Xu, Ying-Chun1
关键词: Trichosporon ; reverse line blot hybridization assay ; rolling circle amplification
刊名: MEDICAL MYCOLOGY
发表日期: 2013-04-01
DOI: 10.3109/13693786.2012.723223
卷: 51, 期:3, 页:300-308
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Mycology ; Veterinary Sciences
研究领域[WOS]: Mycology ; Veterinary Sciences
关键词[WOS]: MOLECULAR-IDENTIFICATION ; ANTIFUNGAL SUSCEPTIBILITIES ; INTERGENIC SPACER-1 ; CLINICAL SPECIMENS ; CANDIDA ; DNA ; MALIGNANCIES ; ASPERGILLUS ; INFECTIONS ; SPP.
英文摘要:

We developed a reverse line blot (RLB) hybridization-, and rolling circle amplification (RCA)-based assays for the identification of Trichoporon species and evaluated them with 48 isolates that had been previously recognized as belonging to eight species (Trichosporon asahii, T. cutaneum, T. dermatis, T. domesticum, T. inkin, T. japonicum, T. jirovecii, and T. laibachii). Results were compared to those obtained with DNA sequencing of three rRNA gene loci, i.e., the internal transcribed spacer (ITS) region, D1/D2 domain of the 28S rRNA gene and intergenic spacer 1 (IGS1) region. Using species-specific, or group-specific probes targeted at the ITS region and the D1/D2 domain, the RLB assay permitted accurate species identification of all 48 isolates with 100% specificity. Species-specific RLB probes correctly assigned 45/48 (94%) of the isolates (six species) with the exception of T. dermatis and T. japonicum isolates which were not targeted by the assay. Identification of T. dermatis relied on a positive hybridization result with the group-specific probe hybridizing with T. dermatis and T. jirovecii and the absence of a signal with the T. jirovecii-specific probe. T. japonicum strains were first assigned to the T. asahii-T. japonicum group by hybridization with the two species group-specific probe and then as T. japonicum by the absence of signal with a T. asahii specific probe. Twelve species-specific RCA probes targeting the eight species studied detected templates of all 48 Trichosporon isolates and an artificial template of T. asteroides, all with good specificity. Both RLB and RCA are potential alternatives to DNA sequencing for the identification of Trichosporon species. The RLB approach is suited for the batched simultaneous analysis of large numbers of isolates, while RCA is more appropriate for the immediate study of single isolates. Comparative costs are US$7 and US$2 per assay for the RLB and RCA methods, respectively.

语种: 英语
所属项目编号: 2009ZX10004-102 ; 200802026
项目资助者: National Science Research Megaproject against Major Infectious Diseases in China ; Ministry of Health of the People&prime ; s Republic of China
WOS记录号: WOS:000316228100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64229
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Clin Lab, Beijing 100730, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China
3.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
4.Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing 100871, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Dept Clin Lab, Affiliated Hosp 1, Beijing, Peoples R China

Recommended Citation:
Xiao, Meng,Guo, Li-Na,Kong, Fanrong,et al. Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA)[J]. MEDICAL MYCOLOGY,2013,51(3):300-308.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xiao, Meng]'s Articles
[Guo, Li-Na]'s Articles
[Kong, Fanrong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xiao, Meng]‘s Articles
[Guo, Li-Na]‘s Articles
[Kong, Fanrong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace