IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA)
Xiao, Meng1,2; Guo, Li-Na1; Kong, Fanrong3; Wang, He1; Sorrell, Tania C.3; Li, Ruo-Yu4; Jiang, Wei5; Chen, Sharon C-A.3; Xu, Ying-Chun1
关键词Trichosporon reverse line blot hybridization assay rolling circle amplification
刊名MEDICAL MYCOLOGY
2013-04-01
DOI10.3109/13693786.2012.723223
51期:3页:300-308
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology ; Veterinary Sciences
研究领域[WOS]Mycology ; Veterinary Sciences
关键词[WOS]MOLECULAR-IDENTIFICATION ; ANTIFUNGAL SUSCEPTIBILITIES ; INTERGENIC SPACER-1 ; CLINICAL SPECIMENS ; CANDIDA ; DNA ; MALIGNANCIES ; ASPERGILLUS ; INFECTIONS ; SPP.
英文摘要

We developed a reverse line blot (RLB) hybridization-, and rolling circle amplification (RCA)-based assays for the identification of Trichoporon species and evaluated them with 48 isolates that had been previously recognized as belonging to eight species (Trichosporon asahii, T. cutaneum, T. dermatis, T. domesticum, T. inkin, T. japonicum, T. jirovecii, and T. laibachii). Results were compared to those obtained with DNA sequencing of three rRNA gene loci, i.e., the internal transcribed spacer (ITS) region, D1/D2 domain of the 28S rRNA gene and intergenic spacer 1 (IGS1) region. Using species-specific, or group-specific probes targeted at the ITS region and the D1/D2 domain, the RLB assay permitted accurate species identification of all 48 isolates with 100% specificity. Species-specific RLB probes correctly assigned 45/48 (94%) of the isolates (six species) with the exception of T. dermatis and T. japonicum isolates which were not targeted by the assay. Identification of T. dermatis relied on a positive hybridization result with the group-specific probe hybridizing with T. dermatis and T. jirovecii and the absence of a signal with the T. jirovecii-specific probe. T. japonicum strains were first assigned to the T. asahii-T. japonicum group by hybridization with the two species group-specific probe and then as T. japonicum by the absence of signal with a T. asahii specific probe. Twelve species-specific RCA probes targeting the eight species studied detected templates of all 48 Trichosporon isolates and an artificial template of T. asteroides, all with good specificity. Both RLB and RCA are potential alternatives to DNA sequencing for the identification of Trichosporon species. The RLB approach is suited for the batched simultaneous analysis of large numbers of isolates, while RCA is more appropriate for the immediate study of single isolates. Comparative costs are US$7 and US$2 per assay for the RLB and RCA methods, respectively.

语种英语
WOS记录号WOS:000316228100009
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64229
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Clin Lab, Beijing 100730, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China
3.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
4.Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing 100871, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Dept Clin Lab, Affiliated Hosp 1, Beijing, Peoples R China
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GB/T 7714
Xiao, Meng,Guo, Li-Na,Kong, Fanrong,et al. Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA)[J]. MEDICAL MYCOLOGY,2013,51(3):300-308.
APA Xiao, Meng.,Guo, Li-Na.,Kong, Fanrong.,Wang, He.,Sorrell, Tania C..,...&Xu, Ying-Chun.(2013).Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA).MEDICAL MYCOLOGY,51(3),300-308.
MLA Xiao, Meng,et al."Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA)".MEDICAL MYCOLOGY 51.3(2013):300-308.
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