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学科主题: 临床医学
题名:
Human Adipose Derived Stem Cells Induced Cell Apoptosis and S Phase Arrest in Bladder Tumor
作者: Yu, Xi1,2,3; Su, Boxing1,2,3; Ge, Peng1,2,3; Wang, Zicheng1,2,3; Li, Sen4; Huang, Bingwei5; Gong, Yanqing1,2,3; Lin, Jian1,2,3
刊名: STEM CELLS INTERNATIONAL
发表日期: 2015
DOI: 10.1155/2015/619290
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering
研究领域[WOS]: Cell Biology
关键词[WOS]: STROMAL CELLS ; CYTOCHROME-C ; CANCER-CELLS ; CYCLE ARREST ; BONE-MARROW ; IN-VIVO ; TISSUE ; GROWTH ; MITOCHONDRIA ; CARCINOMA
英文摘要:

The aim of this study was to determine the effect of human adipose derived stem cells (ADSCs) on the viability and apoptosis of human bladder cancer cells. EJ and T24 cells were cocultured with ADSCs or cultured with conditioned medium of ADSCs (ADSC-CM), respectively. The cell counting and colony formation assay showed ADSCs inhibited the proliferation of EJ and T24 cells. Cell viability assessment revealed that the secretions of ADSCs, in the form of conditioned medium, were able to decrease cancer cell viability. Wound-healing assay suggested ADSC-CM suppressed migration of T24 and EJ cells. Moreover, the results of the flow cytometry indicated that ADSC-CM was capable of inducing apoptosis of T24 cells and inducing S phase cell cycle arrest. Western blot revealed ADSC-CM increased the expression of cleaved caspase-3 and cleaved PARP, indicating thatADSC-CM induced apoptosis in a caspase-dependent way. PTEN/PI3K/Akt pathway and Bcl-2 family proteinswere involved in themechanism of this reaction. Our study indicated that ADSCs may provide a promising and practicable manner for bladder tumor therapy.

语种: 英语
所属项目编号: WJP-LC-12036
项目资助者: Wu Jieping Medical Foundation
WOS记录号: WOS:000349179100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64242
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Beijing Shunyi Dist Hosp, Beijing 101300, Peoples R China
2.Qinzhou First Peoples Hosp, Qinzhou 535000, Peoples R China
3.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
4.Natl Urol Canc Ctr, Beijing 100034, Peoples R China
5.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China

Recommended Citation:
Yu, Xi,Su, Boxing,Ge, Peng,et al. Human Adipose Derived Stem Cells Induced Cell Apoptosis and S Phase Arrest in Bladder Tumor[J]. STEM CELLS INTERNATIONAL,2015.
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