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学科主题: 药学
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One single standard substance for the determination of multiple anthraquinone derivatives in rhubarb using high-performance liquid chromatography-diode array detection
作者: Gao, Xiao-Yan1; Jiang, Yong1; Lu, Jian-qiu2; Tu, Peng-Fei1
关键词: Traditional Chinese medicines (TCMs) ; Rhubarb ; Relative response factors ; Simultaneous determination of multiple ; components (SDMC)
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-03-13
DOI: 10.1016/j.chroma.2008.11.104
卷: 1216, 期:11, 页:2118-2123
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: MOBILE-PHASE ; THIN-LAYER ; ELECTROPHORESIS ; BILOBALIDE
英文摘要:

Due to the complexity of the chemical constitution of traditional Chinese medicines (TCMs), now there is a trend to establish methods, using multi-components analysis, for the effective quality control of TCMs. However, the limited availability Of Multiple reference substances hinders the wide popularization for routine quality control of TCMs. Using an easily available single component contained in the analyte as an internal standard to determine multiple analogues should be a practical option. In this study, we selected rhubarb rhizome as an example, and used emodin, the easily available active component, as the external standard to determine directly the content of emodin in rhubarb, and the same component as the internal standard to simultaneously determine the other six anthraquinones in rhubarb. Compared with the results obtained using the external standard method, this alternative method was found to have no statistically significant differences in our laboratory as verified by the F-test (p = 95%, n = 6). However, due to weak robustness caused by the fluctuation of relative response factors in different laboratories, such a method can only serve as a practical measure to solve the lack of so many chemical standard substances when relative response factors have been determined. This alternative method can then be used without reference substances. Once the corresponding chemical standards are available and are acceptable as well as cost-effective, then the external standard method for the simultaneous determination of multiple components (SDMC) in TCMs will prevail. (C) 2008 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 20070410441 ; 2004-N-103-02
项目资助者: China Postdoctoral Science Foundation ; Natural Research Foundation of Qinghai Province
WOS记录号: WOS:000264226500018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64250
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Beijing Univ Chinese Med, Sci Dev Ctr Tradit Chinese Med, Beijing 100029, Peoples R China

Recommended Citation:
Gao, Xiao-Yan,Jiang, Yong,Lu, Jian-qiu,et al. One single standard substance for the determination of multiple anthraquinone derivatives in rhubarb using high-performance liquid chromatography-diode array detection[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2118-2123.
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