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TRBV kinetics and its association with HLA disparity and aGVHD following allogeneic hematopoietic stem cell transplantation
Sun, L.; Ren, H.; Liu, W.; Wang, L.; Yin, Y.; Li, Y.; Ma, X.; Qiu, Z.; Cen, X.; Shi, Y.
关键词TCR beta variable human leukocyte antigen disparity allogeneic hematopoietic stem cell transplantation
刊名INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
2013-04-01
DOI10.1111/ijlh.12007
35期:2页:119-127
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology
资助者National Natural Science Foundation of China ; China National Key Technology RD Program ; National Natural Science Foundation of China ; China National Key Technology RD Program
研究领域[WOS]Hematology
关键词[WOS]VERSUS-HOST-DISEASE ; BONE-MARROW-TRANSPLANTATION ; RECEPTOR ALPHA-CHAIN ; IMMUNE RECONSTITUTION ; PERIPHERAL-BLOOD ; T-CELLS ; UNMANIPULATED GRAFTS ; MOLECULAR ANALYSIS ; LEUKEMIA PATIENTS ; REPERTOIRE
英文摘要

Introduction The relative expression of T cell receptor (TCR) beta variable (TRBV) and TCR diversity was compared between recipients receiving human leukocyte antigen (HLA)-mismatched transplants and those receiving HLA-matched transplants, using granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood stem cells and bone marrow as grafts. Methods The kinetics of the relative expression of TRBV family members were analyzed using real-time quantitative PCR. Additionally, the association of TRBV clonotype with acute graft-versus-host disease (aGVHD) was determined by cloning and sequence analysis of the CDR3 region. Results The TCR diversity in recipients receiving HLA-mismatched transplants was significantly lower than in those receiving HLA-matched transplants at 1month and 2months after hematopoietic stem cell transplantation (HSCT) (both P<0.05). However, these differences disappeared 3months after transplantation. The relative expression of TRBV27 (n=7 recipients) at the onset of aGVHD was higher than in corresponding donors (P=0.025), but no significant differences were observed between recipients lacking aGVHD and their donors at serial time points after HSCT. Conclusion Our results suggest that HLA disparity may affect the relative expression of TRBV in the early phase after transplantation, and TRBV27 may be associated with the onset of aGVHD.

语种英语
所属项目编号30940030 ; 81070448 ; 2008BAI61B01
资助者National Natural Science Foundation of China ; China National Key Technology RD Program ; National Natural Science Foundation of China ; China National Key Technology RD Program
WOS记录号WOS:000316146600002
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64259
专题北京大学第一临床医学院_血液内科
作者单位Peking Univ, Dept Hematol, Hosp 1, Beijing 100034, Peoples R China
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Sun, L.,Ren, H.,Liu, W.,et al. TRBV kinetics and its association with HLA disparity and aGVHD following allogeneic hematopoietic stem cell transplantation[J]. INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY,2013,35(2):119-127.
APA Sun, L..,Ren, H..,Liu, W..,Wang, L..,Yin, Y..,...&Shi, Y..(2013).TRBV kinetics and its association with HLA disparity and aGVHD following allogeneic hematopoietic stem cell transplantation.INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY,35(2),119-127.
MLA Sun, L.,et al."TRBV kinetics and its association with HLA disparity and aGVHD following allogeneic hematopoietic stem cell transplantation".INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY 35.2(2013):119-127.
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