|Anti-tumor effect of Liqi, a traditional Chinese medicine prescription, in tumor bearing mice|
|Ji, Deng-Bo1; Ye, Jia1; Jiang, Yi-Min2; Qian, Bo-Wen3|
|刊名||BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE|
|WOS标题词||Science & Technology|
|类目[WOS]||Integrative & Complementary Medicine|
|研究领域[WOS]||Integrative & Complementary Medicine|
|关键词[WOS]||NECROSIS-FACTOR ; PONCIRUS-TRIFOLIATA ; IN-VIVO ; THROMBOXANE A(2) ; SAUSSUREA-LAPPA ; CANCER ; CELLS ; METASTASIS ; PROSTACYCLIN ; PLATELETS|
Background: Liqi, an herbal preparation used in traditional Chinese medicine, has been used to treat cancer in China for centuries. We investigated the anti-tumor effects of liqi and their mechanisms in mice that had been xenografted with tumors.
Methods: Sarcoma 180 tumor, Lewis lung carcinoma, and SGC-7901 cells were implanted in BALB/c mice, C57BL/6 mice, and BALB/c nude mice, respectively. Liqi was administered to subgroups of these mice. The tumor weight and size were measured. Cell cycle analysis and T lymphocyte subsets were determined by flow cytometry. The activity of NK cells and TNF was tested using cytotoxicity assay on YAC-1 cells and L929 cells, respectively, and the activity of IL-2 was tested with an IL-2-dependent CTLL-2 cell proliferation assay. Platelet aggregation was monitored by measuring electric impedance, and the levels of thromboxane A2 (TXA(2)) and prostacyclin (PGI(2)) in blood were measured by (125)I-TXB(2) and (125)I-Keto-PGF(1 alpha) radioimmunoassay.
Results: The results showed that liqi inhibited tumor growth in tumor-implanted mice and arrested the cell proliferation in the G0/G1 phase and reduced the portion of cells in S and G2/M phase for SGC-7901 cells. Liqi increased the activity of NK cells and TNF-alpha, stimulated IL-2 production and activity, and regulated T lymphocyte subpopulations. Liqi inhibited the Lewis lung carcinoma metastasis by inhibiting platelet aggregation and normalizing the balance between TXA(2) and PGI(2).
Conclusion: All these findings demonstrated that liqi has an anti-tumor effect in vivo. The mechanism may be related to immune regulation and anticoagulation effects.
|作者单位||1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China|
2.Peking Univ, Med & Healthy Anal Ctr, Beijing 100191, Peoples R China
3.Shanghai Univ Chinese Tradit Med, Dept Tradit Chinese Med, Shanghai 201203, Peoples R China
|Ji, Deng-Bo,Ye, Jia,Jiang, Yi-Min,et al. Anti-tumor effect of Liqi, a traditional Chinese medicine prescription, in tumor bearing mice[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2009,9.|
|APA||Ji, Deng-Bo,Ye, Jia,Jiang, Yi-Min,&Qian, Bo-Wen.(2009).Anti-tumor effect of Liqi, a traditional Chinese medicine prescription, in tumor bearing mice.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,9.|
|MLA||Ji, Deng-Bo,et al."Anti-tumor effect of Liqi, a traditional Chinese medicine prescription, in tumor bearing mice".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 9(2009).|