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学科主题临床医学
Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene
Feng Li-juan; Wan Zhe; Wang Xiao-hong; Li Ruo-yu; Liu Wei
关键词fluconazole Candida albicans ERG11 gene drug resistance
刊名CHINESE MEDICAL JOURNAL
2010-03-05
DOI10.3760/cma.j.issn.0366-6999.2010.05.007
123期:5页:544-548
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]AMINO-ACID SUBSTITUTION ; HIV-INFECTED PATIENTS ; HUMAN-IMMUNODEFICIENCY-VIRUS ; LANOSTEROL 14-ALPHA-DEMETHYLASE ; REDUCED AFFINITY ; AZOLE RESISTANCE ; DRUG-RESISTANCE ; MOLECULAR-MECHANISMS ; 3-DIMENSIONAL MODEL ; STRAINS
英文摘要

Background The cytochrome P450 lanosterol 14 alpha-demethylase (Erg11p) encoded by ERG11 gene is the primary target for azole antifungals. Changes in azole affinity of this enzyme caused by amino acid substitutions have been reported as a mechanism of azole antifungal resistance. This study aimed to investigate the relationship between amino acid substitutions in Erg11p from fluconazole resistant Candida albicans (C. albicans) isolates and their c ross-resistance to azoles.

Methods Mutations in ERG11 gene were screened in 10 clinical isolates of fluconazole resistant C. albicans strains. DNA sequence of ERG11 was determined by PCR based DNA sequencing.

Results In the 10 isolates, 19 types of amino acid substitutions were found, of which 10 substitutions (F72S, F103L, F1451, F198L, G206D, G227D, N349S, F416S, F422L and T482A) have not been reported previously. Mutations in ERG11 gene were detected in 9 isolates of fluconazole resistant C. albicans, but were not detected in 1 isolate.

Conclusions Although no definite correlation was found between the type of amino acid substitutions in Erg11p and the phenotype of cross-resistance to azoles, the substitutions F72S, F1451 and G227D in our study may be highly associated with resistance to azoles because of their special location in Erg11p. Chin Med J 2010;123(5):544-548

语种英语
WOS记录号WOS:000275740200007
项目编号107002
资助机构Key Project of Chinese Ministry of Education
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64280
专题北京大学第一临床医学院_皮肤性病科
北京大学第三临床医学院_药剂科
作者单位Peking Univ, Hosp 1, Dept Dermatol, Res Ctr Med Mycol, Beijing 100034, Peoples R China
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GB/T 7714
Feng Li-juan,Wan Zhe,Wang Xiao-hong,et al. Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene[J]. CHINESE MEDICAL JOURNAL,2010,123(5):544-548.
APA Feng Li-juan,Wan Zhe,Wang Xiao-hong,Li Ruo-yu,&Liu Wei.(2010).Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene.CHINESE MEDICAL JOURNAL,123(5),544-548.
MLA Feng Li-juan,et al."Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene".CHINESE MEDICAL JOURNAL 123.5(2010):544-548.
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