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学科主题: 临床医学
题名:
Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene
作者: Feng Li-juan; Wan Zhe; Wang Xiao-hong; Li Ruo-yu; Liu Wei
关键词: fluconazole ; Candida albicans ; ERG11 gene ; drug resistance
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-03-05
DOI: 10.3760/cma.j.issn.0366-6999.2010.05.007
卷: 123, 期:5, 页:544-548
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: AMINO-ACID SUBSTITUTION ; HIV-INFECTED PATIENTS ; HUMAN-IMMUNODEFICIENCY-VIRUS ; LANOSTEROL 14-ALPHA-DEMETHYLASE ; REDUCED AFFINITY ; AZOLE RESISTANCE ; DRUG-RESISTANCE ; MOLECULAR-MECHANISMS ; 3-DIMENSIONAL MODEL ; STRAINS
英文摘要:

Background The cytochrome P450 lanosterol 14 alpha-demethylase (Erg11p) encoded by ERG11 gene is the primary target for azole antifungals. Changes in azole affinity of this enzyme caused by amino acid substitutions have been reported as a mechanism of azole antifungal resistance. This study aimed to investigate the relationship between amino acid substitutions in Erg11p from fluconazole resistant Candida albicans (C. albicans) isolates and their c ross-resistance to azoles.

Methods Mutations in ERG11 gene were screened in 10 clinical isolates of fluconazole resistant C. albicans strains. DNA sequence of ERG11 was determined by PCR based DNA sequencing.

Results In the 10 isolates, 19 types of amino acid substitutions were found, of which 10 substitutions (F72S, F103L, F1451, F198L, G206D, G227D, N349S, F416S, F422L and T482A) have not been reported previously. Mutations in ERG11 gene were detected in 9 isolates of fluconazole resistant C. albicans, but were not detected in 1 isolate.

Conclusions Although no definite correlation was found between the type of amino acid substitutions in Erg11p and the phenotype of cross-resistance to azoles, the substitutions F72S, F1451 and G227D in our study may be highly associated with resistance to azoles because of their special location in Erg11p. Chin Med J 2010;123(5):544-548

语种: 英语
所属项目编号: 107002
项目资助者: Key Project of Chinese Ministry of Education
WOS记录号: WOS:000275740200007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64280
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Dermatol, Res Ctr Med Mycol, Beijing 100034, Peoples R China

Recommended Citation:
Feng Li-juan,Wan Zhe,Wang Xiao-hong,et al. Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene[J]. CHINESE MEDICAL JOURNAL,2010,123(5):544-548.
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