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学科主题: 临床医学
题名:
Effects of Icariin on Improving Erectile Function in Streptozotocin-Induced Diabetic Rats
作者: Liu, Tao1; Xin, Hua2; Li, Wei-Ren1; Zhou, Feng1; Li, Guang-Yong1; Gong, Yan-Qing1; Gao, Zhe-Zhu1; Qin, Xin-Cheng3; Cui, Wan-Shou1; Shindel, Alan W.4; Xin, Zhong-Cheng1
关键词: Erectile Function ; Diabetes ; Cavernous Nerve Injury ; Corpus Cavernosum ; Nitric Oxide Synthase ; Smooth Muscle ; Endothelium ; Transforming Growth Factor-beta ; Smad
刊名: JOURNAL OF SEXUAL MEDICINE
发表日期: 2011-10-01
DOI: 10.1111/j.1743-6109.2011.02421.x
卷: 8, 期:10, 页:2761-2772
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: CAVERNOUS NERVE INJURY ; NITRIC-OXIDE SYNTHASE ; ENDOTHELIAL-CELLS ; STEM-CELLS ; DYSFUNCTION ; EXPRESSION ; MODEL ; RECOVERY ; MELLITUS ; PATHOPHYSIOLOGY
英文摘要:

Introduction. Icariin has been shown to improve penile hemodynamics in animal models of erectile dysfunction from cavernous nerve injury and castration. The effects of icariin on penile hemodynamics in diabetic animals remain to be determined. Transforming growth factor beta 1 (TGF beta 1) has been implicated in the pathogenesis of diabetes-related erectile dysfunction.

Aim. The aim of this study was to investigate the effects of icariin in the penis of streptozotocin (STZ)-induced diabetic rat.

Methods. Two-month-old Sprague-Dawley male rats received one-time intraperitoneal (IP) STZ (60 mg/kg) or vehicle injection after a 16-hour fast. Three days later, the STZ-induced diabetic rats were randomly divided into four groups and were treated with daily gavage feedings of a 50: 50 mix of normal saline and dimethyl sulfoxide (DMSO) or icariin dissolved in DMSO at doses of 1, 5, and 10 mg/kg for 3 months. A positive control group underwent IP injection of saline followed by daily gavage of saline/DMSO solution. Treatment was stopped 1 week prior to functional assay and euthanasia.

Main Outcome Measure. Penile hemodynamics was assessed by electrical stimulation of the cavernous nerves with real-time intracavernous pressure (ICP) measurement. After euthanasia, penile tissue was studied using immunohistochemistry, Western blot, and enzyme-linked immunosorbent assay (ELISA) to assess the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) and TGF beta 1/Smad2 signaling pathway.

Results. Diabetes attenuated ICP response in control animals. Untreated diabetic animals had decreased smooth muscle/collagen ratio and endothelial cell content in the corpora cavernosa; treatment with icariin partially attenuating these effects. Icariin-treated animals also had a significantly greater expression of nicotinamide adenine dinucleotide phosphate-positive nerves and the endothelial cell markers, von Willebrand factor (vWF), and platelet endothelial cell adhesion molecule-1 (PECAM). TGF beta 1/Smad2 signaling pathway was down-regulated in the penis from icariin-treated models relative to what was observed in negative control animals.

Conclusion. Icariin treatment preserved penile hemodynamics, smooth muscle and endothelial integrity, and neuronal nitric oxide synthase expression in the penis of diabetic rats. Down-regulation of TGF beta 1/Smad2 signaling pathway might mediate this effect. Liu T, Xin H, Li W-R, Zhou F, Li G-Y, Gong Y-Q, Gao Z-Z, Qin X-C, Cui W-S, Shindel AW, and Xin Z-C. Effects of icariin on improving erectile function in streptozotocin-induced diabetic rats. J Sex Med 2011; 8: 2761-2772.

语种: 英语
所属项目编号: 30972993
项目资助者: National Natural Science Foudation of China
WOS记录号: WOS:000295874900010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64287
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Androl Ctr, Hosp 1, Beijing 100871, Peoples R China
2.Chonbuk Natl Univ Hosp, Dept Ophthalmol, Jeonju, South Korea
3.Chinese Dis Control & Prevent Ctr, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China
4.Univ Calif Davis, Dept Urol, Sacramento, CA 95817 USA

Recommended Citation:
Liu, Tao,Xin, Hua,Li, Wei-Ren,et al. Effects of Icariin on Improving Erectile Function in Streptozotocin-Induced Diabetic Rats[J]. JOURNAL OF SEXUAL MEDICINE,2011,8(10):2761-2772.
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