|Vesicular Glutamate Transporter-3 Contributes to Visceral Hyperalgesia Induced by Trichinella spiralis Infection in Rats|
|Yang, Chang-qing1; Wei, Yan-yu1; Leng, Yu-xin1; Zhong, Chan-juan1; Zhang, Yong-shen1; Wan, You2; Duan, Li-ping1|
|关键词||Vesicular glutamate transporter-3 Visceral hyperalgesia Trichinella spiralis Stress Rat|
|刊名||DIGESTIVE DISEASES AND SCIENCES|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||IRRITABLE-BOWEL-SYNDROME ; PAIN ; HYPERSENSITIVITY ; NEURONS|
According to a recent study, vesicular glutamate transporter-3 (VGLUT3) contributes to injury-induced mechanical hyperalgesia in mice.
The aims of the study were to investigate whether VGLUT3 is involved in visceral pain, and whether transient intestinal infection or acute cold restraint stress (ACRS) affects VGLUT3 expression levels in rats.
Changes in VGLUT3 and c-Fos proteins were evaluated in rats which received noxious colorectal distension (CRD) stimulation. Transient intestinal infection was effected by oral administration of Trichinella spiralis (T. spiralis) larvae in Brown Norway rats. On the 100th day post-infection (PI), half of the PI-rats and non infected controls were subjected to an ACRS procedure. The visceromotor response to CRD was measured using the abdominal withdrawal reflex (AWR) score. Immunofluorescence and western blot analysis were used to estimate the expression of VGLUT3 in both peripheral and central neurons.
Noxious stimulation induced a significant increase in the expression of VGLUT3 in the L6S1 spinal dorsal horn. Compared with the control group, the pain threshold was significantly decreased in the ACRS, PI, and PI + ACRS groups. VGLUT3 expression in the L6S1 dorsal root ganglion (DRG) and spinal neurons were significantly increased in PI and PI + ACRS groups as compared with the control group.
VGLUT3 is involved in conduction of visceral pain sensation and in visceral hyperalgesia induced by Trichinella spiralis infection in rats.
|资助机构||National Natural Science Foundation of China ; Doctoral Program of Higher Education of China|
|作者单位||1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China|
2.Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100191, Peoples R China
|Yang, Chang-qing,Wei, Yan-yu,Leng, Yu-xin,et al. Vesicular Glutamate Transporter-3 Contributes to Visceral Hyperalgesia Induced by Trichinella spiralis Infection in Rats[J]. DIGESTIVE DISEASES AND SCIENCES,2012,57(4):865-872.|
|APA||Yang, Chang-qing.,Wei, Yan-yu.,Leng, Yu-xin.,Zhong, Chan-juan.,Zhang, Yong-shen.,...&Duan, Li-ping.(2012).Vesicular Glutamate Transporter-3 Contributes to Visceral Hyperalgesia Induced by Trichinella spiralis Infection in Rats.DIGESTIVE DISEASES AND SCIENCES,57(4),865-872.|
|MLA||Yang, Chang-qing,et al."Vesicular Glutamate Transporter-3 Contributes to Visceral Hyperalgesia Induced by Trichinella spiralis Infection in Rats".DIGESTIVE DISEASES AND SCIENCES 57.4(2012):865-872.|