IR@PKUHSC  > 北京大学第三临床医学院  > 皮肤科
学科主题临床医学
Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis
Cong, Y. J.1,2; Li, L. F.3
关键词beta-lactoglobulin cow milk allergy epitope critical amino acid
刊名JOURNAL OF DAIRY SCIENCE
2012-11-01
DOI10.3168/jds.2012-5543
95期:11页:6307-6312
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Agriculture, Dairy & Animal Science ; Food Science & Technology
研究领域[WOS]Agriculture ; Food Science & Technology
关键词[WOS]COWS MILK ALLERGY ; HUMAN IGE-BINDING ; ALPHA-LACTALBUMIN ; PEPTIDES ; CHILDREN ; SPECIFICITY ; ANTIBODIES
英文摘要

beta-Lactoglobulin represents one of the major allergens causing cow milk allergy. Few studies have clearly evaluated immunological relationships between IgE- and IgG-binding epitopes of beta-lactoglobulin. For characterization of immunological epitopes, peptides of 15 amino acids (AA) in length were synthesized. Immunoglobulin E- and IgG-binding epitopes were immunolabeled with individual sera from cow milk-allergic patients. Alanine scanning of immunodominant epitopes was used to identify the critical AA of IgE- and IgG-binding epitopes. The results showed that 4 IgE-binding epitopes were identified. Our initial data revealed IgE-binding epitopes at AA 17 to 31, AA 72 to 86, AA 92 to 106, and AA 152 to 166. Threonine 20, Met23, and Asp27 are the critical AA of IgG-binding epitopes. Two IgG epitopes were identified, which were located at AA 22 to 36 and AA 127 to 141. The critical AA of IgG-binding epitopes were Leu26 and Val31. Results obtained from this study will provide necessary information to alter the cDNA to encode a protein capable of activating milk-specific T cells, but with reduced IgE- or IgG-binding capacity.

语种英语
WOS记录号WOS:000310107000014
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64297
专题北京大学第三临床医学院_皮肤科
作者单位1.Beijing Technol & Business Univ, Beijing Key Lab Food Flavor Chem, Beijing 100048, Peoples R China
2.Beijing Technol & Business Univ, Beijing Higher Inst Engn Res Ctr Food Addit & Ing, Beijing 100048, Peoples R China
3.Peking Univ Third Hosp, Dept Dermatol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Cong, Y. J.,Li, L. F.. Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis[J]. JOURNAL OF DAIRY SCIENCE,2012,95(11):6307-6312.
APA Cong, Y. J.,&Li, L. F..(2012).Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis.JOURNAL OF DAIRY SCIENCE,95(11),6307-6312.
MLA Cong, Y. J.,et al."Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis".JOURNAL OF DAIRY SCIENCE 95.11(2012):6307-6312.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cong, Y. J.]的文章
[Li, L. F.]的文章
百度学术
百度学术中相似的文章
[Cong, Y. J.]的文章
[Li, L. F.]的文章
必应学术
必应学术中相似的文章
[Cong, Y. J.]的文章
[Li, L. F.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。