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学科主题: 临床医学
题名:
Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis
作者: Cong, Y. J.1,2; Li, L. F.3
关键词: beta-lactoglobulin ; cow milk allergy ; epitope ; critical amino acid
刊名: JOURNAL OF DAIRY SCIENCE
发表日期: 2012-11-01
DOI: 10.3168/jds.2012-5543
卷: 95, 期:11, 页:6307-6312
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Agriculture, Dairy & Animal Science ; Food Science & Technology
研究领域[WOS]: Agriculture ; Food Science & Technology
关键词[WOS]: COWS MILK ALLERGY ; HUMAN IGE-BINDING ; ALPHA-LACTALBUMIN ; PEPTIDES ; CHILDREN ; SPECIFICITY ; ANTIBODIES
英文摘要:

beta-Lactoglobulin represents one of the major allergens causing cow milk allergy. Few studies have clearly evaluated immunological relationships between IgE- and IgG-binding epitopes of beta-lactoglobulin. For characterization of immunological epitopes, peptides of 15 amino acids (AA) in length were synthesized. Immunoglobulin E- and IgG-binding epitopes were immunolabeled with individual sera from cow milk-allergic patients. Alanine scanning of immunodominant epitopes was used to identify the critical AA of IgE- and IgG-binding epitopes. The results showed that 4 IgE-binding epitopes were identified. Our initial data revealed IgE-binding epitopes at AA 17 to 31, AA 72 to 86, AA 92 to 106, and AA 152 to 166. Threonine 20, Met23, and Asp27 are the critical AA of IgG-binding epitopes. Two IgG epitopes were identified, which were located at AA 22 to 36 and AA 127 to 141. The critical AA of IgG-binding epitopes were Leu26 and Val31. Results obtained from this study will provide necessary information to alter the cDNA to encode a protein capable of activating milk-specific T cells, but with reduced IgE- or IgG-binding capacity.

语种: 英语
所属项目编号: 31101236 ; 2011AA100903 ; KM201110011003
项目资助者: National Natural Science Foundation of China (Beijing, China) ; National Science and Technology Project in Rural Areas ; Beijing Municipal Commission of Education
WOS记录号: WOS:000310107000014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64297
Appears in Collections:北京大学第三临床医学院_皮肤科_期刊论文

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作者单位: 1.Beijing Technol & Business Univ, Beijing Key Lab Food Flavor Chem, Beijing 100048, Peoples R China
2.Beijing Technol & Business Univ, Beijing Higher Inst Engn Res Ctr Food Addit & Ing, Beijing 100048, Peoples R China
3.Peking Univ Third Hosp, Dept Dermatol, Beijing 100083, Peoples R China

Recommended Citation:
Cong, Y. J.,Li, L. F.. Identification of the critical amino acid residues of immunoglobulin E and immunoglobulin G epitopes in beta-lactoglobulin by alanine scanning analysis[J]. JOURNAL OF DAIRY SCIENCE,2012,95(11):6307-6312.
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