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学科主题: 口腔医学
题名:
Identification of a Novel Splicing Form of Amelogenin Gene in a Reptile, Ctenosaura similis
作者: Wang, Xinping1; Deng, Xuliang2; Zhang, Xichen1
刊名: PLOS ONE
发表日期: 2012-09-24
DOI: 10.1371/journal.pone.0045871
卷: 7, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PRIMARY RNA TRANSCRIPT ; MESSENGER-RNAS ; DNA-SEQUENCE ; RAT INCISOR ; CDNA SEQUENCE ; IN-VIVO ; PROTEIN ; ENAMEL ; CLONING ; EXPRESSION
英文摘要:

Amelogenin, the major enamel matrix protein in tooth development, has been demonstrated to play a significant role in tooth enamel formation. Previous studies have identified the alternative splicing of amelogenin in many mammalian vertebrates as one mechanism for amelogenin heterogeneous expression in teeth. While amelogenin and its splicing forms in mammalian vertebrates have been cloned and sequenced, the amelogenin gene, especially its splicing forms in non-mammalian species, remains largely unknown. To better understand the mechanism underlying amelogenin evolution, we previously cloned and characterized an amelogenin gene sequence from a squamate, the green iguana. In this study, we employed RT-PCR to amplify the amelogenin gene from the black spiny-tailed iguana Ctenosaura similis teeth, and discovered a novel splicing form of the amelogenin gene. The transcript of the newly identified iguana amelogenin gene (named C. Similis-T2L) is 873 nucleotides long encoding an expected polypeptide of 206 amino acids. The C. Similis-T2L contains a unique exon denominated exon X, which is located between exon 5 and exon 6. The C. Similis-T2L contains 7 exons including exon 1, 2, 3, 5, X, 6, and 7. Analysis of the secondary and tertiary structures of T2L amelogenin protein demonstrated that exon X has a dramatic effect on the amelogenin structures. This is the first report to provide definitive evidence for the amelogenin alternative splicing in non-mammalian vertebrates, revealing a unique exon X and the splicing form of the amelogenin gene transcript in Ctenosaura similis.

语种: 英语
所属项目编号: 4305050102Q6 ; 4305050102O1 ; 2012CB933904 ; 81171000
项目资助者: Jilin University ; National Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号: WOS:000309554700080
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64302
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Beijing Univ, Sch Stomatol, Beijing 100871, Peoples R China
2.Jilin Univ, Coll Vet Med, Changchun 130023, Jilin, Peoples R China

Recommended Citation:
Wang, Xinping,Deng, Xuliang,Zhang, Xichen. Identification of a Novel Splicing Form of Amelogenin Gene in a Reptile, Ctenosaura similis[J]. PLOS ONE,2012,7(9).
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