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High-throughput salting-out assisted liquid-liquid extraction with acetonitrile for the determination of anandamide in plasma of hemodialysis patients with liquid chromatography tandem mass spectrometry
Xiong, Xin1; Zhang, Lei2; Cheng, Litao3; Mao, Wei2
关键词anandamide salting-out assisted liquid-liquid extraction LC-MS MS hemodialysis patients
刊名BIOMEDICAL CHROMATOGRAPHY
2015-09-01
DOI10.1002/bmc.3425
29期:9页:1317-1324
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]FATTY-ACID ETHANOLAMIDES ; LC-MS/MS ; SAMPLE PREPARATION ; BRAIN ; ENDOCANNABINOIDS ; BIOANALYSIS
英文摘要

Anandamide (AEA) is an endocannabinoid present in human plasma that is associated with several physiological functions and disease states. However, low AEA plasma levels pose challenges in terms of analytical characterization. Classical liquid-based lipid extraction and solid-phase extraction require complicated procedures and the drying down of relatively large volumes of solvents, making them unsuitable for high-throughput analysis. Here a high-throughput salting-out assisted liquid-liquid extraction (SALLE) method with acetonitrile and mass spectrometry compatible salts for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of AEA in human plasma has been developed and validated. The seamless interface of SALLE and LC-MS eliminated the drying-down step, only 100L of plasma is required and minimal volumes of organic solvent are used. Good reproducibility, accuracy and precision were demonstrated during the method validation. The method is linear up to 10ng/mL with a lower limit of quantitation of 0.1ng/mL for AEA, the accuracy for AEA was from 93.3 to 96.7% and the precision was <8.57%. This new methodology was successfully applied to analysis of clinical samples from maintenance hemodialysis patients. Copyright (c) 2015 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000359861400004
项目编号21405007 ; 2012B031800213
资助机构National Natural Science Funds of China ; Guangdong Province Science and Technology Project
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64315
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Nephrol, Beijing 100191, Peoples R China
3.Guangdong Prov Hosp Chinese Med, Dept Nephrol, Guangzhou 510120, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Xiong, Xin,Zhang, Lei,Cheng, Litao,et al. High-throughput salting-out assisted liquid-liquid extraction with acetonitrile for the determination of anandamide in plasma of hemodialysis patients with liquid chromatography tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(9):1317-1324.
APA Xiong, Xin,Zhang, Lei,Cheng, Litao,&Mao, Wei.(2015).High-throughput salting-out assisted liquid-liquid extraction with acetonitrile for the determination of anandamide in plasma of hemodialysis patients with liquid chromatography tandem mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,29(9),1317-1324.
MLA Xiong, Xin,et al."High-throughput salting-out assisted liquid-liquid extraction with acetonitrile for the determination of anandamide in plasma of hemodialysis patients with liquid chromatography tandem mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 29.9(2015):1317-1324.
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