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学科主题: 临床医学
题名:
High-throughput salting-out assisted liquid-liquid extraction with acetonitrile for the determination of anandamide in plasma of hemodialysis patients with liquid chromatography tandem mass spectrometry
作者: Xiong, Xin1; Zhang, Lei2; Cheng, Litao3; Mao, Wei2
关键词: anandamide ; salting-out assisted liquid-liquid extraction ; LC-MS ; MS ; hemodialysis patients
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2015-09-01
DOI: 10.1002/bmc.3425
卷: 29, 期:9, 页:1317-1324
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: FATTY-ACID ETHANOLAMIDES ; LC-MS/MS ; SAMPLE PREPARATION ; BRAIN ; ENDOCANNABINOIDS ; BIOANALYSIS
英文摘要:

Anandamide (AEA) is an endocannabinoid present in human plasma that is associated with several physiological functions and disease states. However, low AEA plasma levels pose challenges in terms of analytical characterization. Classical liquid-based lipid extraction and solid-phase extraction require complicated procedures and the drying down of relatively large volumes of solvents, making them unsuitable for high-throughput analysis. Here a high-throughput salting-out assisted liquid-liquid extraction (SALLE) method with acetonitrile and mass spectrometry compatible salts for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of AEA in human plasma has been developed and validated. The seamless interface of SALLE and LC-MS eliminated the drying-down step, only 100L of plasma is required and minimal volumes of organic solvent are used. Good reproducibility, accuracy and precision were demonstrated during the method validation. The method is linear up to 10ng/mL with a lower limit of quantitation of 0.1ng/mL for AEA, the accuracy for AEA was from 93.3 to 96.7% and the precision was <8.57%. This new methodology was successfully applied to analysis of clinical samples from maintenance hemodialysis patients. Copyright (c) 2015 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 21405007 ; 2012B031800213
项目资助者: National Natural Science Funds of China ; Guangdong Province Science and Technology Project
WOS记录号: WOS:000359861400004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64315
Appears in Collections:北京大学第三临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Nephrol, Beijing 100191, Peoples R China
3.Guangdong Prov Hosp Chinese Med, Dept Nephrol, Guangzhou 510120, Guangdong, Peoples R China

Recommended Citation:
Xiong, Xin,Zhang, Lei,Cheng, Litao,et al. High-throughput salting-out assisted liquid-liquid extraction with acetonitrile for the determination of anandamide in plasma of hemodialysis patients with liquid chromatography tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(9):1317-1324.
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