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学科主题: 口腔医学
题名:
Apoptosis induced by atRA in MEPM cells is mediated through activation of caspase and RAR
作者: Yu, ZL; Han, J; Lin, JX; Xiao, Y; Zhang, XZ; Li, Y
关键词: all-trans retinoic acid ; mouse embryonic palatal mesenchymal cells ; apoptosis ; caspase ; retinoic acid receptor
刊名: TOXICOLOGICAL SCIENCES
发表日期: 2006-02-01
DOI: 10.1093/toxsci/kfj046
卷: 89, 期:2, 页:504-509
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: RETINOIC ACID RECEPTOR ; MITOCHONDRIAL CONTROL ; GROWTH-INHIBITION ; CYTOCHROME-C ; CANCER CELLS ; MOUSE ; INDUCTION ; LIGANDS ; LIMB ; DIFFERENTIATION
英文摘要:

We have previously demonstrated that all-trans retinoic (atRA) induced growth inhibition and apoptosis in mouse embryonic palate mesenchymal cells (MEPM). In the present study, we investigated the molecular mechanisms of atRA-induced apoptosis and its putative action pathway. atRA-induced apoptosis is associated with activation of the initiator caspase-9 and the effector caspase-3, but not of the effector caspase-8. A broad caspase inhibitor (z-VAD-fmk), caspase-9 inhibitor z-LEHD-fmk and caspase-3 inhibitor (z-DEVD-fmk) blocked atRA-induced DNA fragmentation and sub-G1 fraction, but not caspase-8 inhibitor z-IETD-fmk. We further showed that atRA dose-dependently promoted mRNA expression of retinoic acid receptor beta (RAR-beta) and gamma. A weaker increase in RAR-alpha mRNA was seen only at the highest concentration of atRA (5 mu M). The pan RAR antagonist, BMS493, completely abrogated atRA-induced DNA fragmentation, Sub-G1 fraction, and caspase-3 activation. Taken together, these findings show that caspase-mediated induction of apoptosis by atRA is an RAR-dependent signaling pathway.

语种: 英语
WOS记录号: WOS:000234631000019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64320
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Beijing 100081, Peoples R China

Recommended Citation:
Yu, ZL,Han, J,Lin, JX,et al. Apoptosis induced by atRA in MEPM cells is mediated through activation of caspase and RAR[J]. TOXICOLOGICAL SCIENCES,2006,89(2):504-509.
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