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Involvement of system A in the retina-to-blood transport of L-proline across the inner blood-retinal barrier
Yoneyama, Daisuke1; Shinozaki, Yumiko1; Lu, Wan-Liang1,2; Tomi, Masatoshi1; Tachikawa, Masanori1; Hosoya, Ken-ichi1
关键词blood-retinal barrier retinal capillary endothelial cells L-proline system A retina-to-blood transport
刊名EXPERIMENTAL EYE RESEARCH
2010-04-01
DOI10.1016/j.exer.2010.01.003
90期:4页:507-513
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]AMINO-ACID TRANSPORTER ; TISSUE EXPRESSION PATTERN ; EFFLUX TRANSPORT ; FUNCTIONAL-CHARACTERISTICS ; GLUTAMATE RELEASE ; GENE-EXPRESSION ; CELL-LINES ; L-CYSTINE ; BRAIN ; RAT
英文摘要

The purpose of the present study was to elucidate the mechanisms of retina-to-blood transport of L-proline across the blood retinal barrier (BRB) in vivo and in vitro, and to identify the responsible transporter(s). The vitreous humor/retina-to-blood transport of [(3)H]L-proline across the BRB was evaluated by microdialysis. Transport mechanisms of [(3)H]L-proline were investigated by cellular uptake using an in vitro model of the inner BRB (TR-iBRB2 cells). The mRNA level of system A was determined by quantitative real-time PCR analysis with specific primers. [(3)H]L-Proline and [(14)C]D-mannitol, which is a bulk flow marker, were bi-exponentially eliminated from the vitreous humor after vitreous bolus injection. The elimination rate constant of [(3)H]L-proline during the terminal phase was 1.6-fold greater than that of [(14)C]D-mannitol. The terminal elimination rate constant difference between [(3)H]L-proline and [(14)C]D-mannitol was reduced in the retinal presence of 3 mM L-proline and 5 mM a-methylaminoisobutyric acid, suggesting that L-proline is transported via a carrier-mediated retina-to-blood transport process across the BRB. [(3)H]L-Proline uptake by TR-iBRB2 cells appeared to be mediated through a saturable and Na(+)-dependent process. The corresponding Michaelis-Menten constant was 392 mu M. This process was reduced by substrates for system A, suggesting that system A is involved in L-proline uptake. Of the isoforms of system A, ATA1, ATA2, and ATA3, ATA2 mRNA is predominantly expressed in TR-iBRB2 cells and isolated rat retinal endothelial cells. In conclusion, system A, most likely ATA2, is responsible for the retina-to-blood transport of c-proline across the inner BRB and may play a role in maintaining the concentration of small neutral amino acids in the retina. (C) 2010 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000276757100005
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64329
Collection北京大学药学院
作者单位1.Toyama Univ, Grad Sch Med & Pharmaceut Sci, Dept Pharmaceut, Toyama 9300194, Japan
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yoneyama, Daisuke,Shinozaki, Yumiko,Lu, Wan-Liang,et al. Involvement of system A in the retina-to-blood transport of L-proline across the inner blood-retinal barrier[J]. EXPERIMENTAL EYE RESEARCH,2010,90(4):507-513.
APA Yoneyama, Daisuke,Shinozaki, Yumiko,Lu, Wan-Liang,Tomi, Masatoshi,Tachikawa, Masanori,&Hosoya, Ken-ichi.(2010).Involvement of system A in the retina-to-blood transport of L-proline across the inner blood-retinal barrier.EXPERIMENTAL EYE RESEARCH,90(4),507-513.
MLA Yoneyama, Daisuke,et al."Involvement of system A in the retina-to-blood transport of L-proline across the inner blood-retinal barrier".EXPERIMENTAL EYE RESEARCH 90.4(2010):507-513.
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