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学科主题: 基础医学
题名:
Localization of protein kinase C isoforms in the optic pathway of mouse embryos and their role in axon routing at the optic chiasm
作者: Wang, Liqing1,2; Lam, Joyce Shi-Ying1; Zhao, Hui1; Wang, Jun3; Chan, Sun-On1
关键词: Development ; Retina ; Visual pathway ; Chiasm ; Optic tract
刊名: BRAIN RESEARCH
发表日期: 2014-08-05
DOI: 10.1016/j.brainres.2014.05.027
卷: 1575, 页:22-32
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: CHONDROITIN SULFATE PROTEOGLYCANS ; RETINOFUGAL PATHWAY ; ENZYMATIC REMOVAL ; IMMUNOHISTOCHEMICAL LOCALIZATION ; POSTNATAL-DEVELOPMENT ; CONFOCAL MICROSCOPY ; NEURITE OUTGROWTH ; GROWTH CONES ; EXPRESSION ; RETINA
英文摘要:

Protein kinase C (PKC) plays a key role in many receptor-mediated signaling pathways that regulate cell growth and development. However, its roles in guiding axon growth and guidance in developing neural pathways are largely unknown. To investigate possible functions of PKC in the growth and guidance of axons in the optic chasm, we first determined the localization of major PKC isoforms in the retinofugal pathway of mouse embryos, at the stage when axons navigate through the midline. Results showed that PKC was expressed in isoform specific patterns in the pathway. PKC-alpha immunoreactivity was detected in the chiasm and the optic tract. PKC-beta II was strong in the optic stalk but was attenuated on axons in the diencephalon. Immunostaining for PKC-epsilon showed a colocalization in the chiasmatic neurons that express a surface antigen stage specific embryonic antigen-1 (SSEA-1). These chiasmatic neurons straddled the midline of the optic chiasm, and have been shown in earlier studies a role in regulation of axon growth and guidance. Expression levels of PKC-beta I, -delta and -gamma were barely detectable in the pathway. Blocking of PKC signaling with Ro-32-0432, an inhibitor specific for PKC-alpha and beta at nanomolar concentration, produced a dramatic reduction of ipsilateral axons from both nasal retina and temporal crescent. We conclude from these studies that PKC-alpha and -beta II are the predominant forms in the developing optic pathway, whereas PKC-epsilon is the major form in the chiasmatic neurons. Furthermore, PKC-alpha and -beta II are likely involved in signaling pathways triggered by inhibitory molecules at the midline that guide optic axons to the uncrossed pathway. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 461709 ; CUHK461612
项目资助者: Earmarked Grant from Research Grants Council, University Grants Committee (RGC) ; General Research Fund from the Hong Kong Special Administrative Region Government
WOS记录号: WOS:000340322800003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64330
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
2.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Neurol, Guangzhou 510275, Guangdong, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Anat & Embryol, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Liqing,Lam, Joyce Shi-Ying,Zhao, Hui,et al. Localization of protein kinase C isoforms in the optic pathway of mouse embryos and their role in axon routing at the optic chiasm[J]. BRAIN RESEARCH,2014,1575:22-32.
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