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学科主题口腔医学
Adhesion of monocytes to periodontal fibroblasts requires activation of NOD1/2-and TLR4-mediated LFA-1 and VLA-4
Liu, Jianru1; Liu, Wenyi1; Xie, Ying1; Wang, Yixiang2; Ouyang, Xiangying1
关键词Adhesion NOD1/2 TLR4 Monocyte Periodontal fibroblast
刊名ARCHIVES OF ORAL BIOLOGY
2015-06-01
DOI10.1016/j.archoralbio.2014.12.012
60期:6页:834-844
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]HUMAN GINGIVAL FIBROBLASTS ; TOLL-LIKE RECEPTOR-2 ; NF-KAPPA-B ; PORPHYROMONAS-GINGIVALIS ; RHEUMATOID-ARTHRITIS ; MONONUCLEAR-CELLS ; GENE-EXPRESSION ; MOLECULES ; INTERLEUKIN-8 ; TOLL-LIKE-RECEPTOR-4
英文摘要

Objective: The aim of this study was to investigate the roles of nucleotide-binding oligomerization domain-containing protein 1/2 (NOD1/2) and Toll-like receptor 4 (TLR4) in mediating the adhesion of monocytes to periodontal fibroblasts through leucocyte function-associated antigen-1 (LFA-1) and very late antigen-4 (VLA-4).

Design: The expression of NOD1, NOD2, and TLR4 was detected in the gingival tissue of patients with chronic periodontitis by immunohistochemistry. Then the adhesion of cells of human monocytic cell line U937 to human gingival fibroblasts (hGFs) and human periodontal ligament cells (hPDLCs) was investigated after U937 cells were treated with the agonists of NOD1, NOD2, and TLR4 for 24h, or transfected with small interfering RNAs (siRNAs) targeting NOD1, NOD2, and TLR4 for 48h. Meanwhile, the expression of LFA-1 and VLA-4 was examined in U937 cells through real-time polymerase chain reaction (PCR), Western blot, and flow cytometry. To confirm the roles of LFA-1 and VLA-4 involved in the process of adhesion, the adhesion blockade assay was performed using the corresponding blocking antibodies against these adhesion molecules.

Results: The immunostaining results showed that NOD1, NOD2, and TLR4 were highly expressed in the gingival tissue of patients with periodontitis, especially in the monocyte-infiltrated area. The activation of these receptors by agonists upregulated the expression of LFA-1 and VLA-4 in U937 cells, and it increased the affinity of U937 cells to hGFs or hPDLCs. On the other hand, knockdown of these receptors by specific siRNAs resulted in the opposite results. In addition, blocking either LFA-1 or VLA-4 in U937 cells significantly attenuated the agonist-triggered adhesion of U937 to periodontal fibroblasts (P<0.001).

Conclusions: These results suggested that NOD1/2 and TLR4 mediated monocyte-periodontal fibroblast adhesion via the modulation of LFA-1 and VLA-4. (C) 2014 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000355355300006
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64331
专题北京大学口腔医学院_牙周科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Liu, Jianru,Liu, Wenyi,Xie, Ying,et al. Adhesion of monocytes to periodontal fibroblasts requires activation of NOD1/2-and TLR4-mediated LFA-1 and VLA-4[J]. ARCHIVES OF ORAL BIOLOGY,2015,60(6):834-844.
APA Liu, Jianru,Liu, Wenyi,Xie, Ying,Wang, Yixiang,&Ouyang, Xiangying.(2015).Adhesion of monocytes to periodontal fibroblasts requires activation of NOD1/2-and TLR4-mediated LFA-1 and VLA-4.ARCHIVES OF ORAL BIOLOGY,60(6),834-844.
MLA Liu, Jianru,et al."Adhesion of monocytes to periodontal fibroblasts requires activation of NOD1/2-and TLR4-mediated LFA-1 and VLA-4".ARCHIVES OF ORAL BIOLOGY 60.6(2015):834-844.
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