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IR@PKUHSC  > 北京大学口腔医学院  > 牙周科  > 期刊论文
学科主题: 口腔医学
题名:
Adhesion of monocytes to periodontal fibroblasts requires activation of NOD1/2-and TLR4-mediated LFA-1 and VLA-4
作者: Liu, Jianru1; Liu, Wenyi1; Xie, Ying1; Wang, Yixiang2; Ouyang, Xiangying1
关键词: Adhesion ; NOD1/2 ; TLR4 ; Monocyte ; Periodontal fibroblast
刊名: ARCHIVES OF ORAL BIOLOGY
发表日期: 2015-06-01
DOI: 10.1016/j.archoralbio.2014.12.012
卷: 60, 期:6, 页:834-844
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: HUMAN GINGIVAL FIBROBLASTS ; TOLL-LIKE RECEPTOR-2 ; NF-KAPPA-B ; PORPHYROMONAS-GINGIVALIS ; RHEUMATOID-ARTHRITIS ; MONONUCLEAR-CELLS ; GENE-EXPRESSION ; MOLECULES ; INTERLEUKIN-8 ; TOLL-LIKE-RECEPTOR-4
英文摘要:

Objective: The aim of this study was to investigate the roles of nucleotide-binding oligomerization domain-containing protein 1/2 (NOD1/2) and Toll-like receptor 4 (TLR4) in mediating the adhesion of monocytes to periodontal fibroblasts through leucocyte function-associated antigen-1 (LFA-1) and very late antigen-4 (VLA-4).

Design: The expression of NOD1, NOD2, and TLR4 was detected in the gingival tissue of patients with chronic periodontitis by immunohistochemistry. Then the adhesion of cells of human monocytic cell line U937 to human gingival fibroblasts (hGFs) and human periodontal ligament cells (hPDLCs) was investigated after U937 cells were treated with the agonists of NOD1, NOD2, and TLR4 for 24h, or transfected with small interfering RNAs (siRNAs) targeting NOD1, NOD2, and TLR4 for 48h. Meanwhile, the expression of LFA-1 and VLA-4 was examined in U937 cells through real-time polymerase chain reaction (PCR), Western blot, and flow cytometry. To confirm the roles of LFA-1 and VLA-4 involved in the process of adhesion, the adhesion blockade assay was performed using the corresponding blocking antibodies against these adhesion molecules.

Results: The immunostaining results showed that NOD1, NOD2, and TLR4 were highly expressed in the gingival tissue of patients with periodontitis, especially in the monocyte-infiltrated area. The activation of these receptors by agonists upregulated the expression of LFA-1 and VLA-4 in U937 cells, and it increased the affinity of U937 cells to hGFs or hPDLCs. On the other hand, knockdown of these receptors by specific siRNAs resulted in the opposite results. In addition, blocking either LFA-1 or VLA-4 in U937 cells significantly attenuated the agonist-triggered adhesion of U937 to periodontal fibroblasts (P<0.001).

Conclusions: These results suggested that NOD1/2 and TLR4 mediated monocyte-periodontal fibroblast adhesion via the modulation of LFA-1 and VLA-4. (C) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 181271150
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000355355300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64331
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Jianru,Liu, Wenyi,Xie, Ying,et al. Adhesion of monocytes to periodontal fibroblasts requires activation of NOD1/2-and TLR4-mediated LFA-1 and VLA-4[J]. ARCHIVES OF ORAL BIOLOGY,2015,60(6):834-844.
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