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学科主题: 基础医学
题名:
Protective Effects of Urinary Trypsin Inhibitor on Vascular Permeability Following Subarachnoid Hemorrhage in a Rat Model
作者: Zhou, Ning1; Xu, Ting2; Bai, Ying1; Prativa, Sherchan3; Xu, Jia-Zhou2,4; Li, Kai5; Han, Hong-Bin5; Yan, Jun-Hao2
关键词: Magnetic resonance imaging ; Rat ; Subarachnoid hemorrhage ; Urinary trypsin inhibitor ; Vascular permeability
刊名: CNS NEUROSCIENCE & THERAPEUTICS
发表日期: 2013-09-01
DOI: 10.1111/cns.12122
卷: 19, 期:9, 页:659-666
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences ; Pharmacology & Pharmacy
研究领域[WOS]: Neurosciences & Neurology ; Pharmacology & Pharmacy
关键词[WOS]: BLOOD-BRAIN-BARRIER ; ISCHEMIA/REPERFUSION INJURY ; PROTEASE INHIBITORS ; OXIDATIVE STRESS ; APOPTOSIS ; EDEMA ; ULINASTATIN ; ACTIVATION ; ISCHEMIA ; INFLAMMATION
英文摘要:

Aims Inflammation and apoptosis play important roles in increasing vascular permeability following subarachnoid hemorrhage (SAH). The objective of this study was to evaluate whether urinary trypsin inhibitor (UTI), a serine protease inhibitor, attenuates vascular permeability by its antiinflammatory and antiapoptotic effects after experimental SAH. Methods Subarachnoid hemorrhage models were established in adult male Sprague-Dawley rats by endovascular perforation. UTI was administered by intraperitoneal injection immediately following SAH. Brain edema was assessed by magnetic resonance imaging (MRI) at 24h after SAH. Neurological deficits, brain water content, vascular permeability, malondialdehyde (MDA) concentration, and myeloperoxidase (MPO) activity were evaluated. Immunohistochemical staining and Western blot were used to explore the underlying protective mechanism of UTI. Results Urinary trypsin inhibitor 50,000U/kg significantly attenuated brain edema and neurological deficits and reduced vascular permeability at 24h after SAH. MDA concentration and MPO activity in hippocampus were significantly decreased with UTI treatment. Furthermore, the levels of phosphorylated JNK, NF-B (p65), tumor necrosis factor- (TNF-), interleukin-6 (IL-6) and proapoptotic protein p53, caspase-3 were elevated in the microvascular endothelial cells of the hippocampus after SAH, which were alleviated with UTI treatment. Conclusion Urinary trypsin inhibitor reduced vascular permeability after SAH through its antiinflammatory and antiapptotic effects via blocking the activity of JNK, NF-B, and p53.

语种: 英语
所属项目编号: 01201002
项目资助者: Techpool Research Fund
WOS记录号: WOS:000323252200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64343
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Jishuitan Hosp, Dept Intens Care Unit, Beijing, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Anat & Histol, Beijing 100191, Peoples R China
3.Loma Linda Univ, Sch Med, Dept Physiol & Pharmacol, Loma Linda, CA USA
4.Zunyi Med Coll, Dept Anat, Zunyi, Peoples R China
5.Peking Univ, Hosp 3, Beijing Key Lab MRI Device & Technol, Dept Radiol, Beijing 100191, Peoples R China

Recommended Citation:
Zhou, Ning,Xu, Ting,Bai, Ying,et al. Protective Effects of Urinary Trypsin Inhibitor on Vascular Permeability Following Subarachnoid Hemorrhage in a Rat Model[J]. CNS NEUROSCIENCE & THERAPEUTICS,2013,19(9):659-666.
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