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学科主题基础医学
Protective Effects of Urinary Trypsin Inhibitor on Vascular Permeability Following Subarachnoid Hemorrhage in a Rat Model
Zhou, Ning1; Xu, Ting2; Bai, Ying1; Prativa, Sherchan3; Xu, Jia-Zhou2,4; Li, Kai5; Han, Hong-Bin5; Yan, Jun-Hao2
关键词Magnetic resonance imaging Rat Subarachnoid hemorrhage Urinary trypsin inhibitor Vascular permeability
刊名CNS NEUROSCIENCE & THERAPEUTICS
2013-09-01
DOI10.1111/cns.12122
19期:9页:659-666
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences ; Pharmacology & Pharmacy
研究领域[WOS]Neurosciences & Neurology ; Pharmacology & Pharmacy
关键词[WOS]BLOOD-BRAIN-BARRIER ; ISCHEMIA/REPERFUSION INJURY ; PROTEASE INHIBITORS ; OXIDATIVE STRESS ; APOPTOSIS ; EDEMA ; ULINASTATIN ; ACTIVATION ; ISCHEMIA ; INFLAMMATION
英文摘要

Aims Inflammation and apoptosis play important roles in increasing vascular permeability following subarachnoid hemorrhage (SAH). The objective of this study was to evaluate whether urinary trypsin inhibitor (UTI), a serine protease inhibitor, attenuates vascular permeability by its antiinflammatory and antiapoptotic effects after experimental SAH. Methods Subarachnoid hemorrhage models were established in adult male Sprague-Dawley rats by endovascular perforation. UTI was administered by intraperitoneal injection immediately following SAH. Brain edema was assessed by magnetic resonance imaging (MRI) at 24h after SAH. Neurological deficits, brain water content, vascular permeability, malondialdehyde (MDA) concentration, and myeloperoxidase (MPO) activity were evaluated. Immunohistochemical staining and Western blot were used to explore the underlying protective mechanism of UTI. Results Urinary trypsin inhibitor 50,000U/kg significantly attenuated brain edema and neurological deficits and reduced vascular permeability at 24h after SAH. MDA concentration and MPO activity in hippocampus were significantly decreased with UTI treatment. Furthermore, the levels of phosphorylated JNK, NF-B (p65), tumor necrosis factor- (TNF-), interleukin-6 (IL-6) and proapoptotic protein p53, caspase-3 were elevated in the microvascular endothelial cells of the hippocampus after SAH, which were alleviated with UTI treatment. Conclusion Urinary trypsin inhibitor reduced vascular permeability after SAH through its antiinflammatory and antiapptotic effects via blocking the activity of JNK, NF-B, and p53.

语种英语
WOS记录号WOS:000323252200004
项目编号01201002
资助机构Techpool Research Fund
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64343
专题北京大学第三临床医学院_麻醉科
北京大学基础医学院
北京大学公共卫生学院_公共卫生学院
作者单位1.Jishuitan Hosp, Dept Intens Care Unit, Beijing, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Anat & Histol, Beijing 100191, Peoples R China
3.Loma Linda Univ, Sch Med, Dept Physiol & Pharmacol, Loma Linda, CA USA
4.Zunyi Med Coll, Dept Anat, Zunyi, Peoples R China
5.Peking Univ, Hosp 3, Beijing Key Lab MRI Device & Technol, Dept Radiol, Beijing 100191, Peoples R China
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Zhou, Ning,Xu, Ting,Bai, Ying,et al. Protective Effects of Urinary Trypsin Inhibitor on Vascular Permeability Following Subarachnoid Hemorrhage in a Rat Model[J]. CNS NEUROSCIENCE & THERAPEUTICS,2013,19(9):659-666.
APA Zhou, Ning.,Xu, Ting.,Bai, Ying.,Prativa, Sherchan.,Xu, Jia-Zhou.,...&Yan, Jun-Hao.(2013).Protective Effects of Urinary Trypsin Inhibitor on Vascular Permeability Following Subarachnoid Hemorrhage in a Rat Model.CNS NEUROSCIENCE & THERAPEUTICS,19(9),659-666.
MLA Zhou, Ning,et al."Protective Effects of Urinary Trypsin Inhibitor on Vascular Permeability Following Subarachnoid Hemorrhage in a Rat Model".CNS NEUROSCIENCE & THERAPEUTICS 19.9(2013):659-666.
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