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学科主题临床医学
Different expression of dectin-1 and Toll-like receptor 2 in the lungs of different immune status mice infected with Aspergillus fumigatus
Yang Jian-xun; Liu Wan; Lu Qiao-yun; Wan Zhe; Wang Xiao-hong; Li Ruo-yu
关键词Aspergillus fumigatus dectin-1 Toll-like receptor 2 real-time polymerase chain reaction
刊名CHINESE MEDICAL JOURNAL
2009-09-05
DOI10.3760/cma.j.issn.0366-6999.2009.17.011
122期:17页:2017-2021
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]BETA-GLUCAN RECEPTOR ; PULMONARY ASPERGILLOSIS ; MURINE MACROPHAGES ; CANDIDA-ALBICANS ; FUNGAL-INFECTION ; RECOGNITION ; INNATE ; CYCLOPHOSPHAMIDE ; TOLL-LIKE-RECEPTOR-2 ; RESPONSES
英文摘要

Background Invasive pulmonary aspergillosis (IPA) is a severe and frequently fatal disease in patients receiving treatment with immunosuppressive agents such as cyclophosphamide. Aspergillus fumigatus (A. fumigatus) now is a leading cause of IPA. Dectin-1 and Toll-like receptor 2 (TLR2) are important pattern recognition receptors involved in immune responses to A. fumigatus in vitro. However, the expression of the two receptors during the infection of A. fumigatus in vivo is not completely understood. The effects of cyclophosphamide treatment on the expression of the receptors need to be further studied.

Methods We established different immune status in mice models with or without A. fumigatus infection. On days 1, 3 and 5 post inoculation, pulmonary tissues from mice of the different groups were harvested. Dectin-1 and TLR2 mRNA expression in the lungs of the mice were investigated by real-time PCR. The pulmonary fungal burden in the mice with A. fumigatus infection was also evaluated.

Results In the immunocompetent mice, three days after A. fumigatus inoculation, dectin-1 and TLR2 expression increased markedly compared with the normal control group. Cyclophosphamide inhibited the clearance of pathogens and the expression of dectin-1 with or without A. fumigatus infection in the lungs as well. There was no statistical difference in TLR2 expression between the different immune status groups.

Conclusions Our results suggest that in vivo, dectin-1 and TLR2 are activated during the experimental period which would provide a broad range of possibilities for a specific and effective inflammatory response to kill A. fumigatus. Inhibition of dectin-1 expression may be one of the mechanisms of cyclophosphamide in the development of IPA. Chin Med J 2009;122(17):2017-2021

语种英语
WOS记录号WOS:000269783700011
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64349
专题北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Hosp 1, Dept Dermatol, Res Ctr Med Mycol, Beijing 100034, Peoples R China
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Yang Jian-xun,Liu Wan,Lu Qiao-yun,et al. Different expression of dectin-1 and Toll-like receptor 2 in the lungs of different immune status mice infected with Aspergillus fumigatus[J]. CHINESE MEDICAL JOURNAL,2009,122(17):2017-2021.
APA Yang Jian-xun,Liu Wan,Lu Qiao-yun,Wan Zhe,Wang Xiao-hong,&Li Ruo-yu.(2009).Different expression of dectin-1 and Toll-like receptor 2 in the lungs of different immune status mice infected with Aspergillus fumigatus.CHINESE MEDICAL JOURNAL,122(17),2017-2021.
MLA Yang Jian-xun,et al."Different expression of dectin-1 and Toll-like receptor 2 in the lungs of different immune status mice infected with Aspergillus fumigatus".CHINESE MEDICAL JOURNAL 122.17(2009):2017-2021.
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