IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Isolation of diallyl trisulfide inducible differentially expressed genes in human gastric cancer cells by modified cDNA representational difference analysis
Li, Y; Lu, YY
刊名DNA AND CELL BIOLOGY
2002-11-01
21期:11页:771-780
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Genetics & Heredity
关键词[WOS]ALLYL SULFIDES ; STOMACH-CANCER ; TUMOR CELLS ; G-PROTEIN ; IDENTIFICATION ; CLONING ; RISK ; TRANSCRIPTS
英文摘要

Extensive epidemiologic studies indicated protective effects of consumption of garlic on reducing human gastric cancer (HGC) incidence. Diallyl trisulfide (DATS), a critical organic allyl sulfur component of garlic, was reported to have chemopreventive effects in inhibiting tumor process. We used DATS to treat HGC cell line BGC823 cells, and showed that DATS induces G1/S arrest and apoptosis in BGC823 cells demonstrated by a flow cytometric analysis. To further isolate DATS inducible differentially expressed genes in BGC823 cells, we combined a highly specific subtractive hybridization of cDNA representational difference analysis (cDNA RDA) with a sensitive bidirectional radioactive detection of mRNA differential display (mRNA DD) to develop a subtractive hybridization differential display (SHDD) method. This modified method adopted a first round of bidirectional subtractive hybridization between two sample cDNAs and a second round of bidirectional subtractive hybridization between the two resultant first-round difference products. Bidirectional subtractive hybridizations magnified the differences between the two sample cDNAs and favored isolating mRNA species with very small expression differences. We employed the SHDD method to detect DATS inducible differentially expressed genes in BGC823 cells. A total of 14 cDNA fragments (11 upregulated and 3 downregulated by DATS treatment) were isolated and confirmed by reverse Northern blot analysis. Our data show that SHDD is a powerful technique for identifying differentially expressed mRNA species between two sample cDNAs and provide useful cellular and molecular information for understanding the effects of garlic against human gastric cancer.

语种英语
WOS记录号WOS:000179451700001
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64350
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing 100034, Peoples R China
2.Beijing Inst Canc Res, Beijing Mol Oncol Lab, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Li, Y,Lu, YY. Isolation of diallyl trisulfide inducible differentially expressed genes in human gastric cancer cells by modified cDNA representational difference analysis[J]. DNA AND CELL BIOLOGY,2002,21(11):771-780.
APA Li, Y,&Lu, YY.(2002).Isolation of diallyl trisulfide inducible differentially expressed genes in human gastric cancer cells by modified cDNA representational difference analysis.DNA AND CELL BIOLOGY,21(11),771-780.
MLA Li, Y,et al."Isolation of diallyl trisulfide inducible differentially expressed genes in human gastric cancer cells by modified cDNA representational difference analysis".DNA AND CELL BIOLOGY 21.11(2002):771-780.
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