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学科主题: 临床医学
题名:
L-arginine impacts pulmonary vascular structure in rats with an aortocaval shunt
作者: Wei, B; Du, JB; Qi, JQ; Li, J; Tang, CS
关键词: L-arginine ; shunt ; proliferation ; apoptosis
刊名: JOURNAL OF SURGICAL RESEARCH
发表日期: 2002-11-01
DOI: 10.1006/jsre.2002.6523
卷: 108, 期:1, 页:20-31
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Surgery
研究领域[WOS]: Surgery
关键词[WOS]: SMOOTH-MUSCLE CELLS ; NITRIC-OXIDE ; ENDOTHELIAL-CELLS ; APOPTOSIS ; HYPERTENSION ; DYSFUNCTION ; INCREASES ; SURGERY ; FLOW
英文摘要:

Background. The present study was designed to explore the therapeutic effect of L-arginine on the proliferation and apoptosis of pulmonary artery smooth muscle cells (SMCs) in high pulmonary blood flow-induced pulmonary hypertension and therefore to provide a basis for the mechanism by which L-arginine regulated pulmonary hypertension.

Materials and methods. Twenty-one male SD rats were randomly divided into shunting group, shunting with L-arginine group, and control group. Abdominal aorta and inferior vena cava shunting was produced in rats of the shunting group and the shunting with L-arginine group. Pulmonary artery mean pressure (mPAP) and pulmonary vascular microstructure were analyzed. Immunohistochemistry for proliferative cell nuclear antigen (PCNA) and Fas expressions and TdT-mediated dUTP-biotin nick-end labeling (TUNEL) were used to detect cell proliferation and apoptosis, respectively.

Results. mPAP, RV/BW, and RV/LV + S were significantly increased in shunted rats compared to normal controls (P < 0.01, respectively). Pulmonary vascular structural remodeling developed in shunted rats. Proliferative index (PI), apoptotic index (AI), and the ratio of PI/AI of pulmonary artery SMCs in the rats of shunting group were elevated obviously (P < 0.01). Meanwhile, the expressing integral score of Fas was elevated in the shunting group (P < 0.01). However, L-arginine significantly attenuated pulmonary artery pressure and ameliorated pulmonary vascular structural remodeling. Also, it reduced PI and again augmented AI of pulmonary artery SMCs. The ratio of PI/AI was significantly reduced (P < 0.01). The expressing integral score of Fas was again augmented by L-arginine (P < 0.01).

Conclusion. L-Arginine could inhibit proliferation and promote apoptosis of pulmonary artery SMCs in shunted rats. (C) 2002 Elsevier Science (USA).

语种: 英语
WOS记录号: WOS:000179528000004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64381
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Thorac Surg, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100871, Peoples R China

Recommended Citation:
Wei, B,Du, JB,Qi, JQ,et al. L-arginine impacts pulmonary vascular structure in rats with an aortocaval shunt[J]. JOURNAL OF SURGICAL RESEARCH,2002,108(1):20-31.
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