|L-arginine impacts pulmonary vascular structure in rats with an aortocaval shunt|
|Wei, B; Du, JB; Qi, JQ; Li, J; Tang, CS|
|关键词||L-arginine shunt proliferation apoptosis|
|刊名||JOURNAL OF SURGICAL RESEARCH|
|WOS标题词||Science & Technology|
|关键词[WOS]||SMOOTH-MUSCLE CELLS ; NITRIC-OXIDE ; ENDOTHELIAL-CELLS ; APOPTOSIS ; HYPERTENSION ; DYSFUNCTION ; INCREASES ; SURGERY ; FLOW|
Background. The present study was designed to explore the therapeutic effect of L-arginine on the proliferation and apoptosis of pulmonary artery smooth muscle cells (SMCs) in high pulmonary blood flow-induced pulmonary hypertension and therefore to provide a basis for the mechanism by which L-arginine regulated pulmonary hypertension.
Materials and methods. Twenty-one male SD rats were randomly divided into shunting group, shunting with L-arginine group, and control group. Abdominal aorta and inferior vena cava shunting was produced in rats of the shunting group and the shunting with L-arginine group. Pulmonary artery mean pressure (mPAP) and pulmonary vascular microstructure were analyzed. Immunohistochemistry for proliferative cell nuclear antigen (PCNA) and Fas expressions and TdT-mediated dUTP-biotin nick-end labeling (TUNEL) were used to detect cell proliferation and apoptosis, respectively.
Results. mPAP, RV/BW, and RV/LV + S were significantly increased in shunted rats compared to normal controls (P < 0.01, respectively). Pulmonary vascular structural remodeling developed in shunted rats. Proliferative index (PI), apoptotic index (AI), and the ratio of PI/AI of pulmonary artery SMCs in the rats of shunting group were elevated obviously (P < 0.01). Meanwhile, the expressing integral score of Fas was elevated in the shunting group (P < 0.01). However, L-arginine significantly attenuated pulmonary artery pressure and ameliorated pulmonary vascular structural remodeling. Also, it reduced PI and again augmented AI of pulmonary artery SMCs. The ratio of PI/AI was significantly reduced (P < 0.01). The expressing integral score of Fas was again augmented by L-arginine (P < 0.01).
Conclusion. L-Arginine could inhibit proliferation and promote apoptosis of pulmonary artery SMCs in shunted rats. (C) 2002 Elsevier Science (USA).
|作者单位||1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China|
2.Peking Univ, Hosp 1, Dept Thorac Surg, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100871, Peoples R China
|Wei, B,Du, JB,Qi, JQ,et al. L-arginine impacts pulmonary vascular structure in rats with an aortocaval shunt[J]. JOURNAL OF SURGICAL RESEARCH,2002,108(1):20-31.|
|APA||Wei, B,Du, JB,Qi, JQ,Li, J,&Tang, CS.(2002).L-arginine impacts pulmonary vascular structure in rats with an aortocaval shunt.JOURNAL OF SURGICAL RESEARCH,108(1),20-31.|
|MLA||Wei, B,et al."L-arginine impacts pulmonary vascular structure in rats with an aortocaval shunt".JOURNAL OF SURGICAL RESEARCH 108.1(2002):20-31.|