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学科主题口腔医学
Intracellular Adhesion Molecule-1 Is Regulated by Porphyromonas gingivalis Through Nucleotide Binding Oligomerization Domain-Containing Proteins 1 and 2 Molecules in Periodontal Fibroblasts
Liu, Jianru1; Duan, Jinyu1; Wang, Yixiang2; Ouyang, Xiangying1
关键词Fibroblasts intracellular adhesion molecule-1 NOD1 protein, human NOD2 protein, human periodontitis Porphyromonas gingivalis
刊名JOURNAL OF PERIODONTOLOGY
2014-02-01
DOI10.1902/jop.2013.130152
85期:2页:358-368
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]INNATE IMMUNE-RESPONSES ; TOLL-LIKE RECEPTORS ; ORAL EPITHELIAL-CELLS ; PROINFLAMMATORY CYTOKINES ; INFLAMMATORY RESPONSE ; LIGAMENT FIBROBLASTS ; ENDOTHELIAL-CELLS ; CREVICULAR FLUID ; NOD1 ; EXPRESSION
英文摘要

Background: The mechanism by which Porphyromonas gingivalis regulates intracellular adhesion molecule 1 (ICAM-1) expression in human periodontal ligament cells (hPDLCs) and human gingival fibroblasts (hGFs) is unknown. The aim of this study is to investigate whether nucleotide binding oligomerization domain-containing protein (NOD) 1 and NOD2 are involved in this process and the clinical significance of ICAM-1 in periodontitis.

Methods: hPDLCs and hGFs were treated with P. gingivalis, L-Ala-gamma-D-glutamyl-mesodiaminopimelic acid (an agonist for NOD1), and muramyl dipeptide (an agonist for NOD2). Alternatively, cells transfected with small interfering RNA targeting NOD1 and NOD2 were treated with P. gingivalis. ICAM-1, NOD1, and NOD2 were detected at mRNA and protein levels. In addition, clinical examinations were performed in 30 healthy controls and 40 patients with chronic periodontitis (CP) before and after treatment, and serum-soluble ICAM-1 (sICAM-1) levels in these individuals were detected by enzyme-linked immunosorbent assay.

Results: This study shows that P. gingivalis caused an increase in ICAM-1, NOD1, and NOD2 expression in periodontal fibroblasts. There was a linear correlation between ICAM-1 and NOD1 and NOD2 levels. Activation of NOD1 and NOD2 by the specific agonist led to the upregulation of ICAM-1, whereas knocking down NOD1 and NOD2 caused a reduction in P. gingivalis-induced ICAM-1 production. Furthermore, sICAM-1 levels were higher in patients with CP than in healthy controls and were positively related to the clinical periodontal parameters. After periodontal treatment, sICAM-1 levels decreased significantly.

Conclusions: The present results indicate that sICAM-1 levels are correlated to the severity of periodontitis. NOD1 and NOD2 mediate P. gingivalis-induced ICAM-1 production in periodontal fibroblasts. NOD1 and NOD2 could be considered potential targets for periodontal therapy.

语种英语
所属项目编号181271150
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000331140100021
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64384
Collection北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Dept Cent Lab, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jianru,Duan, Jinyu,Wang, Yixiang,et al. Intracellular Adhesion Molecule-1 Is Regulated by Porphyromonas gingivalis Through Nucleotide Binding Oligomerization Domain-Containing Proteins 1 and 2 Molecules in Periodontal Fibroblasts[J]. JOURNAL OF PERIODONTOLOGY,2014,85(2):358-368.
APA Liu, Jianru,Duan, Jinyu,Wang, Yixiang,&Ouyang, Xiangying.(2014).Intracellular Adhesion Molecule-1 Is Regulated by Porphyromonas gingivalis Through Nucleotide Binding Oligomerization Domain-Containing Proteins 1 and 2 Molecules in Periodontal Fibroblasts.JOURNAL OF PERIODONTOLOGY,85(2),358-368.
MLA Liu, Jianru,et al."Intracellular Adhesion Molecule-1 Is Regulated by Porphyromonas gingivalis Through Nucleotide Binding Oligomerization Domain-Containing Proteins 1 and 2 Molecules in Periodontal Fibroblasts".JOURNAL OF PERIODONTOLOGY 85.2(2014):358-368.
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