北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Generation of novel monoclonal antibodies and their application for detecting ARD1 expression in colorectal cancer
作者: Ren, Tingting1; Jiang, Beihai1; Jin, Genglin1; Li, Jianning1; Dong, Bin2; Zhang, Jianzhi1; Meng, Lin1; Wu, Jian1; Shou, Chengchao1
关键词: ARD1 ; monoclonal antibody ; colorectal cancer
刊名: CANCER LETTERS
发表日期: 2008-06-08
DOI: 10.1016/j.canlet.2008.01.028
卷: 264, 期:1, 页:83-92
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: ACETYLTRANSFERASE COMPLEX ; CELL-CYCLE ; ACETYLATION ; HIF-1-ALPHA ; PROTEIN ; HARD1 ; DESTABILIZATION ; IDENTIFICATION ; HYPOXIA ; GENE
英文摘要:

Arrest defective 1 (ARD1) is an acetyltransferase involved in cell cycle control in yeast. ARD1 interacts with human N-acetyltransferase (NATH) to form a functional N-terminal acetyltransferase complex. Recently it had been linked with proliferation and apoptosis in mammalian cells, but its function in cancer development remains unclear. To evaluate significance of ARD1 expression in human colorectal cancer, we generated a panel of monoclonal antibodies (mAbs) with high specificity and sensitivity against ARD1. All of the 10 different clones could be used in ELISA and Western blot, and clone 10C12, 13G2, and 4D10 can interact with ARD1 in eukaryotic cells by immunoprecipitation (IP). Clones of 14D4 and 10C12 were strongly reacted to ARD1 in immunocytochemistry (ICH) and immunohistochemistry (IHC). ARD1 expression was evaluated in human colorectal cancer and colitis tissues by immunohistochemical analysis with mAb 14D4. Forty-one were ARD1-positive in 50 colorectal cancer tissues and only 12 were weak positive in the 50 matched normal tissues (P < 0.001). Moreover, ARD1 expression was not detectable in 20 cases of colitis tissue (P < 0.001). Furthermore, all of the six human colorectal cancer cell lines we examined were also ARD1-positive at mRNA and protein levels. Taken together, the novel mAbs against ARD1 we generated could be good tools for both basic and clinical studies, and ARD1 could be a potential biomarker in colorectal cancer. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000256011600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64385
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Biochem & Mol Biol, Beijing 100036, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Core Facil Ctr, Beijing 100036, Peoples R China

Recommended Citation:
Ren, Tingting,Jiang, Beihai,Jin, Genglin,et al. Generation of novel monoclonal antibodies and their application for detecting ARD1 expression in colorectal cancer[J]. CANCER LETTERS,2008,264(1):83-92.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ren, Tingting]'s Articles
[Jiang, Beihai]'s Articles
[Jin, Genglin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ren, Tingting]‘s Articles
[Jiang, Beihai]‘s Articles
[Jin, Genglin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace