IR@PKUHSC  > 北京大学第二临床医学院  > 血液科
学科主题临床医学
ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells
Xiao, Yiren1,2; Jiang, Zhiwu1,2; Li, Yin3; Ye, Wei1,2; Jia, Bei4; Zhang, Minjie5; Xu, Yan6,7; Wu, Donghai1,2; Lai, Liangxue1,2; Chen, Yaoyu8; Chang, Yingjun9; Huang, Xiaojun9; Liu, Hudan10; Qing, Guoliang10; Liu, Pentao11; Li, Yangqiu6,7; Xu, Bing3; Zhong, Mei4; Yao, Yao12; Pei, Duanqing1,2; Li, Peng1,2
刊名HAEMATOLOGICA
2015-05-01
DOI10.3324/haematol.2014.118612
100期:5页:585-594
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology
资助者Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Equipment Function Development &amp ; Technology Innovation Project of Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Equipment Function Development &amp ; Technology Innovation Project of Chinese Academy of Sciences
研究领域[WOS]Hematology
关键词[WOS]ANGIOPOIETIN-LIKE PROTEINS ; EX-VIVO EXPANSION ; BONE-MARROW NICHE ; BETA-CATENIN ; SELF-RENEWAL ; CANCER CELLS ; WNT ; DIFFERENTIATION ; QUIESCENCE ; TARGET
英文摘要

Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration.

语种英语
所属项目编号XDA01020310 ; 81272329 ; 81327801 ; 81272211 ; 81200381 ; 81200255 ; 2011CB504004 ; 2010CB945500 ; yg2010080 ; yg2011082 ; yg2012049
资助者Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Equipment Function Development &amp ; Technology Innovation Project of Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Equipment Function Development &amp ; Technology Innovation Project of Chinese Academy of Sciences
WOS记录号WOS:000354788000023
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64386
Collection北京大学第二临床医学院_血液科
作者单位1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Beijing 100864, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Beijing 100864, Peoples R China
3.Southern Med Univ, Nanfang Hosp, Dept Hematol, Guangzhou, Guangdong, Peoples R China
4.Southern Med Univ, Nan Fang Hosp, Dept Obstet & Gynecol, Guangzhou, Guangdong, Peoples R China
5.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Univ Town, Peoples R China
6.Wellcome Trust Sanger Inst, Cambridge, England
7.Jinan Univ, Coll Med, Inst Hematol, Guangzhou, Guangdong, Peoples R China
8.Jinan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
9.Nanjing Med Univ, Dept Hematol, Nanjing, Jiangsu, Peoples R China
10.Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100871, Peoples R China
11.Huazhong Unviers Sci & Technol, Tongji Med Coll, Sch Pharm, Wuhan, Peoples R China
12.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Drug Discovery Pipeline, Beijing 100864, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Yiren,Jiang, Zhiwu,Li, Yin,et al. ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells[J]. HAEMATOLOGICA,2015,100(5):585-594.
APA Xiao, Yiren.,Jiang, Zhiwu.,Li, Yin.,Ye, Wei.,Jia, Bei.,...&Li, Peng.(2015).ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells.HAEMATOLOGICA,100(5),585-594.
MLA Xiao, Yiren,et al."ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells".HAEMATOLOGICA 100.5(2015):585-594.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Xiao, Yiren]'s Articles
[Jiang, Zhiwu]'s Articles
[Li, Yin]'s Articles
百度学术
百度学术Similar articles in
[Xiao, Yiren]'s Articles
[Jiang, Zhiwu]'s Articles
[Li, Yin]'s Articles
必应学术
必应学术Similar articles in
[Xiao, Yiren]'s Articles
[Jiang, Zhiwu]'s Articles
[Li, Yin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.