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学科主题: 临床医学
题名:
ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells
作者: Xiao, Yiren1,2; Jiang, Zhiwu1,2; Li, Yin3; Ye, Wei1,2; Jia, Bei4; Zhang, Minjie5; Xu, Yan6,7; Wu, Donghai1,2; Lai, Liangxue1,2; Chen, Yaoyu8; Chang, Yingjun9; Huang, Xiaojun9; Liu, Hudan10; Qing, Guoliang10; Liu, Pentao11; Li, Yangqiu6,7; Xu, Bing3; Zhong, Mei4; Yao, Yao12; Pei, Duanqing1,2; Li, Peng1,2
刊名: HAEMATOLOGICA
发表日期: 2015-05-01
DOI: 10.3324/haematol.2014.118612
卷: 100, 期:5, 页:585-594
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: ANGIOPOIETIN-LIKE PROTEINS ; EX-VIVO EXPANSION ; BONE-MARROW NICHE ; BETA-CATENIN ; SELF-RENEWAL ; CANCER CELLS ; WNT ; DIFFERENTIATION ; QUIESCENCE ; TARGET
英文摘要:

Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration.

语种: 英语
所属项目编号: XDA01020310 ; 81272329 ; 81327801 ; 81272211 ; 81200381 ; 81200255 ; 2011CB504004 ; 2010CB945500 ; yg2010080 ; yg2011082 ; yg2012049
项目资助者: Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Equipment Function Development &amp ; Technology Innovation Project of Chinese Academy of Sciences
WOS记录号: WOS:000354788000023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64386
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: 1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Beijing 100864, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Beijing 100864, Peoples R China
3.Southern Med Univ, Nanfang Hosp, Dept Hematol, Guangzhou, Guangdong, Peoples R China
4.Southern Med Univ, Nan Fang Hosp, Dept Obstet & Gynecol, Guangzhou, Guangdong, Peoples R China
5.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Univ Town, Peoples R China
6.Wellcome Trust Sanger Inst, Cambridge, England
7.Jinan Univ, Coll Med, Inst Hematol, Guangzhou, Guangdong, Peoples R China
8.Jinan Univ, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
9.Nanjing Med Univ, Dept Hematol, Nanjing, Jiangsu, Peoples R China
10.Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100871, Peoples R China
11.Huazhong Unviers Sci & Technol, Tongji Med Coll, Sch Pharm, Wuhan, Peoples R China
12.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Drug Discovery Pipeline, Beijing 100864, Peoples R China

Recommended Citation:
Xiao, Yiren,Jiang, Zhiwu,Li, Yin,et al. ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells[J]. HAEMATOLOGICA,2015,100(5):585-594.
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