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A non-invasive, rapid method to genotype late-onset Alzheimer′s disease-related apolipoprotein E gene polymorphisms
Yi, Li1; Wu, Ting1; Luo, Wenyuan1; Zhou, Wen2; Wu, Jun1
关键词nerve regeneration neurodegeneration late-onset Alzheimer&prime s disease apolipoprotein E gene real-time PCR DNA sequencing risk factor allele neural regeneration
刊名NEURAL REGENERATION RESEARCH
2014
DOI10.4103/1673-5374.125332
9期:1页:69-75
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Neurosciences
资助者Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality in China ; Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality in China
研究领域[WOS]Cell Biology ; Neurosciences & Neurology
关键词[WOS]TRANSGENIC MOUSE MODEL ; APOE EPSILON-4 ALLELE ; A-BETA ; COGNITIVE IMPAIRMENT ; TYPE-4 ALLELE ; BUCCAL SWABS ; RISK-FACTORS ; GENOMIC DNA ; COLLECTION ; NEUROPATHOLOGY
英文摘要

The apolipoprotein E gene epsilon 4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer′s disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E epsilon 4 allele in neural regeneration in late-onset Alzheimer′s disease cases.

语种英语
所属项目编号201002063 ; JC20110518075 7A
资助者Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality in China ; Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality in China
WOS记录号WOS:000331487500011
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64389
专题北京大学深圳医院_神经内科
作者单位1.Peking Univ, Shenzhen Hosp, Dept Neurol, Shenzhen 518036, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Radiol, Shenzhen 518036, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Yi, Li,Wu, Ting,Luo, Wenyuan,et al. A non-invasive, rapid method to genotype late-onset Alzheimer′s disease-related apolipoprotein E gene polymorphisms[J]. NEURAL REGENERATION RESEARCH,2014,9(1):69-75.
APA Yi, Li,Wu, Ting,Luo, Wenyuan,Zhou, Wen,&Wu, Jun.(2014).A non-invasive, rapid method to genotype late-onset Alzheimer′s disease-related apolipoprotein E gene polymorphisms.NEURAL REGENERATION RESEARCH,9(1),69-75.
MLA Yi, Li,et al."A non-invasive, rapid method to genotype late-onset Alzheimer′s disease-related apolipoprotein E gene polymorphisms".NEURAL REGENERATION RESEARCH 9.1(2014):69-75.
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