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学科主题: 临床医学
题名:
A non-invasive, rapid method to genotype late-onset Alzheimer′s disease-related apolipoprotein E gene polymorphisms
作者: Yi, Li1; Wu, Ting1; Luo, Wenyuan1; Zhou, Wen2; Wu, Jun1
关键词: nerve regeneration ; neurodegeneration ; late-onset Alzheimer&prime ; s disease ; apolipoprotein E gene ; real-time PCR ; DNA sequencing ; risk factor ; allele ; neural regeneration
刊名: NEURAL REGENERATION RESEARCH
发表日期: 2014
DOI: 10.4103/1673-5374.125332
卷: 9, 期:1, 页:69-75
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Neurosciences
研究领域[WOS]: Cell Biology ; Neurosciences & Neurology
关键词[WOS]: TRANSGENIC MOUSE MODEL ; APOE EPSILON-4 ALLELE ; A-BETA ; COGNITIVE IMPAIRMENT ; TYPE-4 ALLELE ; BUCCAL SWABS ; RISK-FACTORS ; GENOMIC DNA ; COLLECTION ; NEUROPATHOLOGY
英文摘要:

The apolipoprotein E gene epsilon 4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer′s disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E epsilon 4 allele in neural regeneration in late-onset Alzheimer′s disease cases.

语种: 英语
所属项目编号: 201002063 ; JC20110518075 7A
项目资助者: Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality in China
WOS记录号: WOS:000331487500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64389
Appears in Collections:北京大学深圳医院_神经内科_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Neurol, Shenzhen 518036, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Radiol, Shenzhen 518036, Guangdong, Peoples R China

Recommended Citation:
Yi, Li,Wu, Ting,Luo, Wenyuan,et al. A non-invasive, rapid method to genotype late-onset Alzheimer′s disease-related apolipoprotein E gene polymorphisms[J]. NEURAL REGENERATION RESEARCH,2014,9(1):69-75.
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