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Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry
Yang, M; Liu, AH; Guan, SH; Sun, JH; Xu, M; Guo, D
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
2006
DOI10.1002/rcm.2447
20期:8页:1266-1280
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Spectroscopy
研究领域[WOS]Chemistry ; Spectroscopy
关键词[WOS]PRESSURE CHEMICAL-IONIZATION ; IN-VITRO CYTOTOXICITY ; DITERPENOIDS ; ODORIFERA ; EXTRACT ; BUNGE
英文摘要

The qualitative analysis of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen in Chinese) was performed using high-performance liquid chromatography with electrospray ionization tandem mass spectrometry (ESI-MSn). Tanshinones are the major bioactive constituents of Dan-shen, which is used in China for the treatment of haematological abnormalities and cardiovascular diseases. The ESI-MSn fragmentation behavior of tanshinones was investigated. For tanshinones with the tanshinone I nucleus, the fragmentation was triggered by loss of a molecule of CO except bearing a substituent at C-17 or C-18, followed by sequential eliminations of CO. If C15-16 was a saturated bond, the fragmentation was triggered by elimination of a molecule of H2O. For tanshinones with the tanshinone IIA nucleus, the fragmentation was triggered by loss of a molecule of H2O, followed by successive eliminations of CO. Ions corresponding to loss of a molecule of propylene (Delta m = 42) were also observed. Moreover, when C15-16 was a saturated bond, ions corresponding to losses of CH3, H2O and propylene were more abundant. If no D-ring existed, the presence of isopropyl resulted in an elimination of a molecule of H2O with an adjacent CO or OR In addition, the extension of the pi-conjugation in the A-ring (especially at C1-2) induced the fragmentation by loss of a molecule of CO. These fragmentation rules were applied to the identification of tanshinones in a chloroform/methanol (3:7) extract of Dan-shen, which was separated on a C18 column with gradient elution. A total of 27 tanshinones were identified, including five new constituents. The established method could be used for the sensitive and rapid identification of tanshinones in the Dan-shen drug and its pharmaceutical preparations. Copyright (c) 2006 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000236852800015
Citation statistics
Cited Times:67[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64390
Collection北京大学药学院
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai 201203, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yang, M,Liu, AH,Guan, SH,et al. Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2006,20(8):1266-1280.
APA Yang, M,Liu, AH,Guan, SH,Sun, JH,Xu, M,&Guo, D.(2006).Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,20(8),1266-1280.
MLA Yang, M,et al."Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 20.8(2006):1266-1280.
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