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Capillary electrochromatography and quartz crystal microbalance, valuable techniques in the study of heparin-lipoprotein interactions
Lipponen, Katriina1; Liu, Yi1,2; Stege, Patricia Wanda1; Oorni, Katariina3; Kovanen, Petri T.3; Riekkola, Marja-Liisa1
关键词Open tubular capillary electrochromatography Quartz crystal microbalance Heparin Low-density lipoproteins High-density lipoproteins Interactions
刊名ANALYTICAL BIOCHEMISTRY
2012-05-01
DOI10.1016/j.ab.2012.02.017
424期:1页:71-78
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]LOW-DENSITY LIPOPROTEINS ; APOLIPOPROTEIN-E ; EXTRACELLULAR-MATRIX ; BINDING-SITES ; ATHEROSCLEROSIS ; PROTEOGLYCANS ; PEPTIDES ; PROTEIN ; GLYCOSAMINOGLYCANS ; ELECTROPHORESIS
英文摘要

Atherosclerosis is initiated when lipoproteins bind to proteoglycans (PGs) in arterial walls. The binding is mediated by apolipoprotein apoB-100 and/or apoE, both of which have binding affinity toward heparin. We developed covalently bound heparin coatings for APTES-modified silica capillaries and SiO2 chips and carried out capillary electrochromatography (CEC) and quartz crystal microbalance (QCM) studies on the interactions of heparin with selected peptide fragments of apoB-100 and apoE and, for CEC, also with low- and high-density lipoproteins (LDL and HDL), the latter with and without apoE. The peptides are known to mediate interactions of HDL and LDL with arterial PGs. Interactions and affinities were expressed in CEC as retention factors and reduced mobilities and in continuous flow QCM techniques as affinity constants. Both techniques showed heparin interactions to be stronger with apoB-100 peptide than with apoE peptide fragment, and they confirmed that the sulfate groups in heparin play an especially important role in interactions with apoB-100 peptide fragments. In addition, CEC confirmed the importance of sulfate groups of heparin in interactions between heparin and LDL and between heparin and apoE-containing HDL. CEC and QCM acted as excellent platforms to mimic these biologically important interactions, with small sample and reagent consumption. (c) 2012 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000302842700012
项目编号116288 ; 1133184
资助机构Research Council for Natural Sciences and Engineering ; Academy of Finland
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64391
专题北京大学第二临床医学院_药剂科
作者单位1.Univ Helsinki, Analyt Chem Lab, Dept Chem, FIN-00014 Helsinki, Finland
2.Peking Univ, Dept Pharm, Peoples Hosp, Beijing 100044, Peoples R China
3.Wihuri Res Inst, FIN-00140 Helsinki, Finland
推荐引用方式
GB/T 7714
Lipponen, Katriina,Liu, Yi,Stege, Patricia Wanda,et al. Capillary electrochromatography and quartz crystal microbalance, valuable techniques in the study of heparin-lipoprotein interactions[J]. ANALYTICAL BIOCHEMISTRY,2012,424(1):71-78.
APA Lipponen, Katriina,Liu, Yi,Stege, Patricia Wanda,Oorni, Katariina,Kovanen, Petri T.,&Riekkola, Marja-Liisa.(2012).Capillary electrochromatography and quartz crystal microbalance, valuable techniques in the study of heparin-lipoprotein interactions.ANALYTICAL BIOCHEMISTRY,424(1),71-78.
MLA Lipponen, Katriina,et al."Capillary electrochromatography and quartz crystal microbalance, valuable techniques in the study of heparin-lipoprotein interactions".ANALYTICAL BIOCHEMISTRY 424.1(2012):71-78.
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