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学科主题: 临床医学
题名:
Capillary electrochromatography and quartz crystal microbalance, valuable techniques in the study of heparin-lipoprotein interactions
作者: Lipponen, Katriina1; Liu, Yi1,2; Stege, Patricia Wanda1; Oorni, Katariina3; Kovanen, Petri T.3; Riekkola, Marja-Liisa1
关键词: Open tubular capillary ; electrochromatography ; Quartz crystal microbalance ; Heparin ; Low-density lipoproteins ; High-density lipoproteins ; Interactions
刊名: ANALYTICAL BIOCHEMISTRY
发表日期: 2012-05-01
DOI: 10.1016/j.ab.2012.02.017
卷: 424, 期:1, 页:71-78
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: LOW-DENSITY LIPOPROTEINS ; APOLIPOPROTEIN-E ; EXTRACELLULAR-MATRIX ; BINDING-SITES ; ATHEROSCLEROSIS ; PROTEOGLYCANS ; PEPTIDES ; PROTEIN ; GLYCOSAMINOGLYCANS ; ELECTROPHORESIS
英文摘要:

Atherosclerosis is initiated when lipoproteins bind to proteoglycans (PGs) in arterial walls. The binding is mediated by apolipoprotein apoB-100 and/or apoE, both of which have binding affinity toward heparin. We developed covalently bound heparin coatings for APTES-modified silica capillaries and SiO2 chips and carried out capillary electrochromatography (CEC) and quartz crystal microbalance (QCM) studies on the interactions of heparin with selected peptide fragments of apoB-100 and apoE and, for CEC, also with low- and high-density lipoproteins (LDL and HDL), the latter with and without apoE. The peptides are known to mediate interactions of HDL and LDL with arterial PGs. Interactions and affinities were expressed in CEC as retention factors and reduced mobilities and in continuous flow QCM techniques as affinity constants. Both techniques showed heparin interactions to be stronger with apoB-100 peptide than with apoE peptide fragment, and they confirmed that the sulfate groups in heparin play an especially important role in interactions with apoB-100 peptide fragments. In addition, CEC confirmed the importance of sulfate groups of heparin in interactions between heparin and LDL and between heparin and apoE-containing HDL. CEC and QCM acted as excellent platforms to mimic these biologically important interactions, with small sample and reagent consumption. (c) 2012 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 116288 ; 1133184
项目资助者: Research Council for Natural Sciences and Engineering ; Academy of Finland
WOS记录号: WOS:000302842700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64391
Appears in Collections:北京大学第二临床医学院_药剂科_期刊论文

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作者单位: 1.Univ Helsinki, Analyt Chem Lab, Dept Chem, FIN-00014 Helsinki, Finland
2.Peking Univ, Dept Pharm, Peoples Hosp, Beijing 100044, Peoples R China
3.Wihuri Res Inst, FIN-00140 Helsinki, Finland

Recommended Citation:
Lipponen, Katriina,Liu, Yi,Stege, Patricia Wanda,et al. Capillary electrochromatography and quartz crystal microbalance, valuable techniques in the study of heparin-lipoprotein interactions[J]. ANALYTICAL BIOCHEMISTRY,2012,424(1):71-78.
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