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学科主题: 临床医学
题名:
FOXL2 suppresses proliferation, invasion and promotes apoptosis of cervical cancer cells
作者: Liu, Xing-Long1,2; Meng, Yu-Han3; Wang, Jian-Li2; Yang, Biao-Bing1; Zhang, Fan1; Tang, Sheng-Jian1
关键词: FOXL2 ; cervical cancer ; proliferation ; apoptosis ; invasiveness
刊名: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
发表日期: 2014
卷: 7, 期:4, 页:1534-1543
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Pathology
研究领域[WOS]: Oncology ; Pathology
关键词[WOS]: TRANSCRIPTION FACTOR FOXL2 ; OVARIAN DEVELOPMENT ; GENE-TRANSCRIPTION ; INVERSUS SYNDROME ; EXPRESSION ; TUMORS ; MUTATION ; ADULT ; C.402C-GREATER-THAN-G ; PROGNOSIS
英文摘要:

FOXL2 is a transcription factor that is essential for ovarian function and maintenance, the germline mutations of which give rise to the blepharophimosis ptosis epicanthus inversus syndrome (BPES), often associated with premature ovarian failure. Recently, its mutations have been found in ovarian granulosa cell tumors (OGCTs). In this study, we measured the expression of FOXL2 in cervical cancer by immunohistochemistry and its mRNA level in cervical cancer cell lines Hela and Siha by RT-PCR. Then we overexpressed FOXL2 in Hela cells and silenced it in Siha cells by plasmid transfection and verified using western blotting. When FOXL2 was overexpressed or silenced, cells proliferation and apoptosis were determined by Brdu assay and Annexin V/PI detection kit, respectively. In addition, we investigated the effects of FOXL2 on the adhesion and invasion of Hela and Siha cells. Finally, we analyzed the influences of FOXL2 on Ki67, PCNA and FasL by flow cytometry. The results showed that FOXL2 was highly expressed in cervical squamous cancer. Overexpressing FOXL2 suppressed Hela proliferation and facilitated its apoptosis. Silencing FOXL2 enhanced Siha proliferation and inhibited its apoptosis. Meanwhile, silencing FOXL2 promoted Siha invasion, but it had no effect on cells adhesion. In addition, overexpressing FOXL2 decreased the expression of Ki67 in Hela and Siha cells. Therefore, our results suggested that FOXL2 restrained cells proliferation and enhanced cells apoptosis mainly through decreasing Ki67 expression.

语种: 英语
所属项目编号: 81272122 ; jk67 ; J09LF20 ; WY20110
项目资助者: National Natural Science Foundation of China ; Science and Technology Projects of Shandong Province ; Science and Technology Plan Projects of Colleges and Universities in Shandong Province ; Weifang Medical University
WOS记录号: WOS:000335227600026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64412
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Weifang Med Univ, Plast Surg Inst, Weifang 261041, Shandong, Peoples R China
2.89th Hosp PLA, Dept Traumat Orthoped, Weifang 261041, Shandong, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Xing-Long,Meng, Yu-Han,Wang, Jian-Li,et al. FOXL2 suppresses proliferation, invasion and promotes apoptosis of cervical cancer cells[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(4):1534-1543.
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