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学科主题: 药学
题名:
Studies on aminoisonucleoside modified siRNAs: Stability and silencing activity
作者: Li, Zong-Sheng; Qiao, Ren-Ping; Du, Quan; Yang, Zhen-Jun; Zhang, Liang-Ren; Zhang, Pei-Zhuo; Liang, Zi-Cai; Zhang, Li-He
刊名: BIOCONJUGATE CHEMISTRY
发表日期: 2007-07-01
DOI: 10.1021/bc060398+
卷: 18, 期:4, 页:1017-1024
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: SMALL INTERFERING RNA ; CHEMICAL-MODIFICATIONS ; MAMMALIAN-CELLS ; IN-VITRO ; OLIGONUCLEOTIDES ; OLIGODEOXYNUCLEOTIDES ; HYBRIDIZATION ; RECOGNITION ; DUPLEXES ; COMPLEX
英文摘要:

A novel class of aminoisonucleoside was synthesized and incorporated into a luciferase gene-targeting siRNA. Structural and functional analyses of such a kind of siRNAs indicated that sense strand modifications with aminoisonucleoside at the 3′ or 5′ terminal, such as ssIso-1 and ssIso-2, have less effect on RNA duplex thermal and serum stabilities, and their functional activities are also comparable to their native siRNAs. In contrast, antisense strand modifications with aminoisonucleoside at the corresponding positions, such as asIso-2 or asIso-1, bring a striking negative effect on RNA duplex stability but still maintain around 40-50% of gene knockdown.

语种: 英语
WOS记录号: WOS:000248085700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64415
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Shanghai GenePharma Co Ltd, ZJ High Tech Pk, Shanghai 201203, Peoples R China

Recommended Citation:
Li, Zong-Sheng,Qiao, Ren-Ping,Du, Quan,et al. Studies on aminoisonucleoside modified siRNAs: Stability and silencing activity[J]. BIOCONJUGATE CHEMISTRY,2007,18(4):1017-1024.
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