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A Potential Role for CPY17 as a Parameter in Differentiation Between Aldosterone-producing Adenoma and Nodular Hyperplasia in Patients With Hyperaldosteronism
Cui, Xijun; Zhao, Lei; Chen, Shuang; Guan, Tianyun; Herrick, Elizabeth J.; Shi, Bingbing; Zang, Meifu; Chen, Songsen; Fang, Yujiang1
刊名UROLOGY
2012-06-01
DOI10.1016/j.urology.2012.02.009
79期:6
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]EXPERIMENTAL AUTOIMMUNE-THYROIDITIS ; SUBCLINICAL CUSHINGS-SYNDROME ; INCREASED EXPRESSION ; ADRENAL ADENOMAS ; STEROIDOGENIC ENZYMES ; RESOLUTION ; CYP11B1
英文摘要

OBJECTIVE To investigate CYP17 mRNA and protein expressions in aldosterone-producing adenoma (APA), nodular hyperplasia (NH) and normal adrenal gland (NAG) and if CPY17 might be used as a potential marker to differentiate between APA and NH in patients with hyperaldosteronism.

METHODS Total RNA and protein were extracted from APA, 12 NH, and 15 NAG tissues. mRNA and protein expressions of CPY17 were examined by real-time polymerase chain reaction (PCR) and Western blot analysis.

RESULTS The relative expression levels of CPY17mRNA to glyceraldehyde 3-phosphate dehydrogenase in the APA, NH, and NAG groups are 0.94 +/- 0.09, 2.07 +/- 0.10, and 3.94 +/- 0.19, respectively, when evaluated by real-time PCR. This result was confirmed by the relative protein expression levels of CPY17 to beta-actin, which are 117 +/- 13%, 274 +/- 19%, and 478 +/- 25%, respectively, when evaluated by Western blot analysis. There was a significant difference in mRNA and protein expression level of CPY17 between any two groups (P < .05). Thus, the sequence of the relative expression level of CPY17 is APA < NH < NAG.

CONCLUSION These results indicate that CPY17 was down-regulated in APA compared with that in NH, suggesting a potential role for CPY17 as a marker in differentiation between APA and NH in patients with hyperaldosteronism. Such a study might be helpful to improve the diagnosis and treatment of primary aldosteronism. UROLOGY 79: 1410.e15-1410.e19, 2012. (C) 2012 Elsevier Inc.

语种英语
WOS记录号WOS:000304720600071
资助机构Department of Surgery, University of Missouri School of Medicine
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64423
专题北京大学第三临床医学院_泌尿外科
作者单位1.Peoples Hosp Ningjin, Dept Surg, Ningjin, Peoples R China
2.Mudanjiang Med Coll, Dept Clin Med, Mudanjiang, Peoples R China
3.Peking Univ, Dept Urol, Hosp 3, Beijing 100871, Peoples R China
4.Univ Missouri, Sch Med, Dept Surg, Ellis Fischel Canc Ctr, Columbia, MO 65212 USA
5.Wannan Med Coll, Affiliated Hosp 2, Dept Internal Med, Wuhu, Peoples R China
6.Pekin Union Med Coll Hosp, Dept Urol, Beijing, Peoples R China
7.Chinese Acad Med Sci, Dept Biochem, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Cui, Xijun,Zhao, Lei,Chen, Shuang,et al. A Potential Role for CPY17 as a Parameter in Differentiation Between Aldosterone-producing Adenoma and Nodular Hyperplasia in Patients With Hyperaldosteronism[J]. UROLOGY,2012,79(6).
APA Cui, Xijun.,Zhao, Lei.,Chen, Shuang.,Guan, Tianyun.,Herrick, Elizabeth J..,...&Fang, Yujiang.(2012).A Potential Role for CPY17 as a Parameter in Differentiation Between Aldosterone-producing Adenoma and Nodular Hyperplasia in Patients With Hyperaldosteronism.UROLOGY,79(6).
MLA Cui, Xijun,et al."A Potential Role for CPY17 as a Parameter in Differentiation Between Aldosterone-producing Adenoma and Nodular Hyperplasia in Patients With Hyperaldosteronism".UROLOGY 79.6(2012).
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