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学科主题: 基础医学
题名:
A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation
作者: Li, Yajuan1; Liu, Shuang2; Lu, Fengmin1; Zhang, Ting1; Chen, Hairu3; Wu, Songwei3; Zhuang, Hui1
关键词: hepatocellular carcinoma ; T-type Ca2+ channel ; proliferation ; Mibefradil
刊名: ONCOLOGY REPORTS
发表日期: 2009-11-01
DOI: 10.3892/or_00000559
卷: 22, 期:5, 页:1229-1235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CALCIUM-CHANNELS ; BREAST-CANCER ; GROWTH ; EXPRESSION ; PATHWAY ; HEPATOCARCINOGENESIS ; PHOSPHORYLATION ; DIFFERENTIATION ; MECHANISMS ; PHENOTYPE
英文摘要:

The role of T-type Ca2+ channels in hepatocellular carcinoma cell proliferation was investigated in vitro. Eleven hepatocellular carcinoma cell lines and one immortalized liver cell line (LO2) were examined for the status of T-type Ca2+ channels with RT-PCR and voltage-clamp recordings. Except HBxF344, other cell lines tested had one, two or all ;three of alpha 1-subunits (alpha 1G, alpha 1H and alpha 11) mRNA expression. Obvious T-type current was recorded in SNU449 cells, while others exhibited a minimal or no T-type current. SNU449, PLC/PRF5, Hep3B and LO2 cell lines were subjected to growth assay in the presence of Mibefradil, a T-type Ca2+ channel blocker, only the proliferation of SNU449 cell which had functional T-type Ca2+ channel was reduced by Mibefradil treatment. Furthermore, the persistent increase of phosphorylated ERK1/2 in SNU449 cells was found when treated with Mibefradil. A microarray assay also demonstrated some down-regulated genes were mainly associated with cell cycle and cell proliferation in Mibefradil treated SNU449 cells. In conclusion, this study showed that the functional T-type Ca2+ channels probably participate in modulating the proliferation of some hepatocellular carcinoma cells. The cell proliferation reduction of SNU449 with Mibefradil treatment is possibly associated with the persistent increase of phosphorylated ERK1/2.

语种: 英语
所属项目编号: 30771099 ; D08050702870000 ; 2008ZX10002-012
项目资助者: National Natural Science Foundation of China ; Project of Beijing Municipal Science and Technology Commission ; National Projects on Major Infectious Diseases, Ministry of Science and Technology of China
WOS记录号: WOS:000270573200037
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64424
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ S Alabama, Dept Pharmacol, Mobile, AL 36688 USA
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
3.Capital Med Univ, Beijing YouAn Hosp, Artificial Liver Treatment & Training Ctr, Beijing 100069, Peoples R China

Recommended Citation:
Li, Yajuan,Liu, Shuang,Lu, Fengmin,et al. A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation[J]. ONCOLOGY REPORTS,2009,22(5):1229-1235.
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