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学科主题: 基础医学
题名:
TRAIL gene reorganizes the cytoskeleton and decreases the motility of human leukemic Jurkat cells
作者: Jiang, Yuhui; Chen, Kai; Tang, Zhiyu; Zeng, Zhu; Yao, Weijuan; Sun, Dagong; Ka, Weibo; He, Dongqi; Wen, Zongyao; Chien, Shu
关键词: tumor necrosis factor-related apoptosis inducing ligand ; Jurkat ; mobility ; deformability ; adhesion ; migration
刊名: CELL MOTILITY AND THE CYTOSKELETON
发表日期: 2006-08-01
DOI: 10.1002/cm.20138
卷: 63, 期:8, 页:471-482
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: APOPTOSIS-INDUCING LIGAND ; MURINE ERYTHROLEUKEMIA-CELLS ; AUTOIMMUNE-DISEASES ; T-CELLS ; ADHESION ; DEATH ; EXPRESSION ; SURFACE ; CANCER ; SHEAR
英文摘要:

TRAIL can selectively induce rapid apoptosis of various types of tumor cells. We induced the expression of TRAIL in Jurkat cells, and measured the adhesion of cells to human umbilical vein endothelial cells (HUVECs) and laminin (LN) in a parallel plate flow chamber system and by using a colorimetric method. The apoptosis percentage, cycle distribution, intracellular Ca2+ concentration, and adhesion molecule expression of the cells were detected by flow cytometry. Cytoskeleton was observed with a laser confocal microscopy. The roles of adhesion molecules in the cell interaction was defined by their function blocking. The results showed that TRAIL attenuated the adhesion of Jurkat cells to HUVECs and LN, as well as their transendothelial migration. The increased apoptosis and G1-phase cell percentages, decreased intracellular Ca2+ concentration, depolymerized actin and impaired cell deformability could contribute to the decreased adhesion of Jurkat cells caused by TRAIL. Furthermore, CD11a was found to play a more important role than CD62L in the adhesion of Jurkat cells to HUVECs. These findings contribute to the knowledge on the role of TRAIL in tumor metastasis and provide mechanistic basis for the clinical application of TRAIL and tumor therapy.

语种: 英语
WOS记录号: WOS:000239225700003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64435
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Guangdong Inst Drug Control, Guangzhou, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Med Math, Beijing 100871, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Med Phys, Beijing 100871, Peoples R China
5.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
6.Univ Calif San Diego, Whitaker Inst Biomed Engn, La Jolla, CA 92093 USA

Recommended Citation:
Jiang, Yuhui,Chen, Kai,Tang, Zhiyu,et al. TRAIL gene reorganizes the cytoskeleton and decreases the motility of human leukemic Jurkat cells[J]. CELL MOTILITY AND THE CYTOSKELETON,2006,63(8):471-482.
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