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学科主题: 临床医学
题名:
Activation of VEGF and ERK1/2 and Improvement of Urethral Function by Adipose-derived Stem Cells in a Rat Stress Urinary Incontinence Model
作者: Li, Guang-Yong; Zhou, Feng; Gong, Yan-Qing; Cui, Wan-Shou; Yuan, Yi-Ming; Song, Wei-Dong; Xin, Hua; Liu, Tao; Li, Wei-Ren; Gao, Zhe-Zhu; Liu, Jing; Guo, Ying-Lu; Xin, Zhong-Cheng
刊名: UROLOGY
发表日期: 2012-10-01
DOI: 10.1016/j.urology.2012.05.030
卷: 80, 期:4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: TISSUE ; WOMEN ; PREVALENCE ; POSTPARTUM ; MANAGEMENT ; ESTROGEN ; THERAPY
英文摘要:

OBJECTIVE To investigate the injected autologous adipose-derived stem cells (ADSCs) in improving stress urinary incontinence in a rodent model of parturition-related stress incontinence and the possible mechanism.

METHODS The 40 rats were developed stress urinary incontinence models by postpartum balloon dilation of the vagina for 4 hours followed by bilateral ovariectomy. ADSCs were isolated from the peri-ovarian fat and labeled with thymidine analog 5-ethynyl-2-deoxyuridine (EdU). Twenty stress urinary incontinence rats received peri-urethral injection of phosphate-buffered saline as the negative controls and the other 20 stress urinary incontinence rats received peri-urethral injection of EdU-labeled ADSCc. Twenty control rats underwent sham ovariectomy without balloon dilation and served as positive controls. Four weeks later, voiding function was assessed by cystometry. Urethral histologic examination (Masson trichrome stain, picrosirius red stain, Hart elastin stain, Gordon and Sweet stain, and immunohistochemical stain) and Western blot were performed on urethral tissues.

RESULTS Both leak point pressure and bladder capacity were significantly increased in ADSC-treated rats, compared to the balloon-injured ovariectomized rats. Histologic examination revealed normalized appearance of the fibromuscular structure of the urethra as well as increased peri-urethral blood vessel density in ADSC-treated rats. On Western blot, vascular endothelial growth factor and P-extracellular signal-regulated kinases (ERKs)1/2 protein was expressed at a higher rate in tissues from ADSC-treated rats compared to phosphate-buffered saline-treated rats.

CONCLUSION Peri-urethral injection of ADSCs is associated with more normal urinary function and urethral structure in rats with parturition-related incontinence. The activation of vascular endothelial growth factor and ERK1/2 may be responsible for the paracrine effects from ADSCs. UROLOGY 80: 953.e1-953.e8, 2012. (C) 2012 Elsevier Inc.

语种: 英语
所属项目编号: Z080507030808011 ; 30972993
项目资助者: Peking National Natural Science Foundation ; National Natural Science Foundation of China
WOS记录号: WOS:000309518700060
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64444
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: Peking Univ, Hosp 1, Androl Ctr, Beijing 100034, Peoples R China

Recommended Citation:
Li, Guang-Yong,Zhou, Feng,Gong, Yan-Qing,et al. Activation of VEGF and ERK1/2 and Improvement of Urethral Function by Adipose-derived Stem Cells in a Rat Stress Urinary Incontinence Model[J]. UROLOGY,2012,80(4).
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