IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1
Liu, Zhiqiang1; Li, Tao1; Liu, Yinan1; Jia, Zhuqing1; Li, Yanming1; Zhang, Chenguang1; Chen, Ping1; Ma, Kangtao1; Affara, Nabeel2; Zhou, Chunyan1
关键词P19CL6 cell Cardiomyogenesis Epigenetic Nkx2.5 HDAC1 WNT
刊名BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
2009-02-01
DOI10.1016/j.bbamcr.2008.08.013
1793期:2页:300-311
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]EMBRYONIC STEM-CELLS ; II HISTONE DEACETYLASES ; CARDIAC DEVELOPMENT ; DEPENDENT RECRUITMENT ; GENE-EXPRESSION ; DNA METHYLATION ; DIFFERENTIATION ; HEART ; MOUSE ; MORPHOGENESIS
英文摘要

The cardiac transcription factor NKX2.5 plays a crucial role in cardiomyogenesis, but its mechanism of regulation is still unclear. Recently, epigenetic regulation has become increasingly recognized as important in differentiation and development. In this study, we used P19CL6 cells to investigate the regulation of Nkx2.5 expression by methylation and acetylation during cardiomyocyte differentiation. During the early stage of differentiation, Nkx2.5 expression was upregulated, but the methylation status of the Nkx2.5 promoter did not undergo significant change; while the acetylation levels of histones H3 and H4 were increased, accompanied by a significant reduction in Hdac1 expression. Suppression of Hdac1 activity stimulated cardiac differentiation accompanied by increased expression of cardiac-specific genes and cell cycle arrest. Overexpression of Hdac1 inhibited cardiomyocyte formation and downregulated the expressions of Gata4 and Nkx2.5. Mimicking induction of the WNT pathway inhibited Hdac1 expression with upregulated Nkx2.5 expression. WNT3a and WNT3 downregulated the expression of Hdac1, contrary to the effect of SFRP2 and GSK3 beta. Cotransfection beta-catenin and Lef1 significantly downregulated the expression of Hdac1. Our data suggest that WNT signaling pathway plays important roles in the regulation of Hdac1 during the early stage of cardiomyocyte differentiation and that the downregulation of Hdac1 promotes cardiac differentiation. (c) 2008 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000263402600009
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64447
Collection北京大学基础医学院
北京大学药学院_药学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
Recommended Citation
GB/T 7714
Liu, Zhiqiang,Li, Tao,Liu, Yinan,et al. WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2009,1793(2):300-311.
APA Liu, Zhiqiang.,Li, Tao.,Liu, Yinan.,Jia, Zhuqing.,Li, Yanming.,...&Zhou, Chunyan.(2009).WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1793(2),300-311.
MLA Liu, Zhiqiang,et al."WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1793.2(2009):300-311.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Liu, Zhiqiang]'s Articles
[Li, Tao]'s Articles
[Liu, Yinan]'s Articles
百度学术
百度学术Similar articles in
[Liu, Zhiqiang]'s Articles
[Li, Tao]'s Articles
[Liu, Yinan]'s Articles
必应学术
必应学术Similar articles in
[Liu, Zhiqiang]'s Articles
[Li, Tao]'s Articles
[Liu, Yinan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.