北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1
作者: Liu, Zhiqiang1; Li, Tao1; Liu, Yinan1; Jia, Zhuqing1; Li, Yanming1; Zhang, Chenguang1; Chen, Ping1; Ma, Kangtao1; Affara, Nabeel2; Zhou, Chunyan1
关键词: P19CL6 cell ; Cardiomyogenesis ; Epigenetic ; Nkx2.5 ; HDAC1 ; WNT
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
发表日期: 2009-02-01
DOI: 10.1016/j.bbamcr.2008.08.013
卷: 1793, 期:2, 页:300-311
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: EMBRYONIC STEM-CELLS ; II HISTONE DEACETYLASES ; CARDIAC DEVELOPMENT ; DEPENDENT RECRUITMENT ; GENE-EXPRESSION ; DNA METHYLATION ; DIFFERENTIATION ; HEART ; MOUSE ; MORPHOGENESIS
英文摘要:

The cardiac transcription factor NKX2.5 plays a crucial role in cardiomyogenesis, but its mechanism of regulation is still unclear. Recently, epigenetic regulation has become increasingly recognized as important in differentiation and development. In this study, we used P19CL6 cells to investigate the regulation of Nkx2.5 expression by methylation and acetylation during cardiomyocyte differentiation. During the early stage of differentiation, Nkx2.5 expression was upregulated, but the methylation status of the Nkx2.5 promoter did not undergo significant change; while the acetylation levels of histones H3 and H4 were increased, accompanied by a significant reduction in Hdac1 expression. Suppression of Hdac1 activity stimulated cardiac differentiation accompanied by increased expression of cardiac-specific genes and cell cycle arrest. Overexpression of Hdac1 inhibited cardiomyocyte formation and downregulated the expressions of Gata4 and Nkx2.5. Mimicking induction of the WNT pathway inhibited Hdac1 expression with upregulated Nkx2.5 expression. WNT3a and WNT3 downregulated the expression of Hdac1, contrary to the effect of SFRP2 and GSK3 beta. Cotransfection beta-catenin and Lef1 significantly downregulated the expression of Hdac1. Our data suggest that WNT signaling pathway plays important roles in the regulation of Hdac1 during the early stage of cardiomyocyte differentiation and that the downregulation of Hdac1 promotes cardiac differentiation. (c) 2008 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000263402600009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64447
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England

Recommended Citation:
Liu, Zhiqiang,Li, Tao,Liu, Yinan,et al. WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2009,1793(2):300-311.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Zhiqiang]'s Articles
[Li, Tao]'s Articles
[Liu, Yinan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Zhiqiang]‘s Articles
[Li, Tao]‘s Articles
[Liu, Yinan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace