IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1
Liu, Zhiqiang1; Li, Tao1; Liu, Yinan1; Jia, Zhuqing1; Li, Yanming1; Zhang, Chenguang1; Chen, Ping1; Ma, Kangtao1; Affara, Nabeel2; Zhou, Chunyan1
关键词P19CL6 cell Cardiomyogenesis Epigenetic Nkx2.5 HDAC1 WNT
刊名BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
2009-02-01
DOI10.1016/j.bbamcr.2008.08.013
1793期:2页:300-311
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]EMBRYONIC STEM-CELLS ; II HISTONE DEACETYLASES ; CARDIAC DEVELOPMENT ; DEPENDENT RECRUITMENT ; GENE-EXPRESSION ; DNA METHYLATION ; DIFFERENTIATION ; HEART ; MOUSE ; MORPHOGENESIS
英文摘要

The cardiac transcription factor NKX2.5 plays a crucial role in cardiomyogenesis, but its mechanism of regulation is still unclear. Recently, epigenetic regulation has become increasingly recognized as important in differentiation and development. In this study, we used P19CL6 cells to investigate the regulation of Nkx2.5 expression by methylation and acetylation during cardiomyocyte differentiation. During the early stage of differentiation, Nkx2.5 expression was upregulated, but the methylation status of the Nkx2.5 promoter did not undergo significant change; while the acetylation levels of histones H3 and H4 were increased, accompanied by a significant reduction in Hdac1 expression. Suppression of Hdac1 activity stimulated cardiac differentiation accompanied by increased expression of cardiac-specific genes and cell cycle arrest. Overexpression of Hdac1 inhibited cardiomyocyte formation and downregulated the expressions of Gata4 and Nkx2.5. Mimicking induction of the WNT pathway inhibited Hdac1 expression with upregulated Nkx2.5 expression. WNT3a and WNT3 downregulated the expression of Hdac1, contrary to the effect of SFRP2 and GSK3 beta. Cotransfection beta-catenin and Lef1 significantly downregulated the expression of Hdac1. Our data suggest that WNT signaling pathway plays important roles in the regulation of Hdac1 during the early stage of cardiomyocyte differentiation and that the downregulation of Hdac1 promotes cardiac differentiation. (c) 2008 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000263402600009
引用统计
被引频次:51[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64447
专题北京大学基础医学院
北京大学药学院_药学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
推荐引用方式
GB/T 7714
Liu, Zhiqiang,Li, Tao,Liu, Yinan,et al. WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2009,1793(2):300-311.
APA Liu, Zhiqiang.,Li, Tao.,Liu, Yinan.,Jia, Zhuqing.,Li, Yanming.,...&Zhou, Chunyan.(2009).WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1793(2),300-311.
MLA Liu, Zhiqiang,et al."WNT signaling promotes Nkx2.5 expression and early cardiomyogenesis via downregulation of Hdac1".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1793.2(2009):300-311.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
WNT signaling promot(1924KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Zhiqiang]的文章
[Li, Tao]的文章
[Liu, Yinan]的文章
百度学术
百度学术中相似的文章
[Liu, Zhiqiang]的文章
[Li, Tao]的文章
[Liu, Yinan]的文章
必应学术
必应学术中相似的文章
[Liu, Zhiqiang]的文章
[Li, Tao]的文章
[Liu, Yinan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: WNT signaling promotes Nkx2_5 expression and early cardiomyogenesis via downregulation of Hdac1.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。