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学科主题: 基础医学
题名:
Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG
作者: Zhao, Hong-xi1; Li, Yang2,3; Jin, Hai-feng4,5; Xie, Li6,7; Liu, Chuang1; Jiang, Feng1; Luo, Ya-ning1; Yin, Guo-wu1; Li, Yi1; Wang, Jun1; Li, Ling-song2,3; Yao, Yuan-qing1,7; Wang, Xiao-hong1
关键词: Reprogramming ; Pluripotency ; Amnion ; Embryonic stem cell
刊名: DIFFERENTIATION
发表日期: 2010-09-01
DOI: 10.1016/j.diff.2010.03.002
卷: 80, 期:2-3, 页:123-129
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Developmental Biology
研究领域[WOS]: Cell Biology ; Developmental Biology
关键词[WOS]: HUMAN SOMATIC-CELLS ; STEM-CELLS ; HUMAN FIBROBLASTS ; EPITHELIAL-CELLS ; DEFINED FACTORS ; GENERATION ; INDUCTION ; THERAPY ; MOUSE ; LINES
英文摘要:

Reprogramming human somatic cells to pluripotency represents a valuable resource for research aiming at the development of in vitro models for human diseases and regenerative medicines to produce patient-specific induced pluripotent stem (iPS) cells. Seeking appropriate cell resources for higher efficiency and reducing the risk of viral transgene activation, especially oncogene activation, are of significance for iPS cell research. In this study, we tested whether human amnion-derived cells (hADCs) could be rapidly and efficiently reprogrammed into iPS cells by the defined factors: OCT4/SOX2/NANOG. hADCs from normal placenta were isolated and cultured. The 3rd passage cells were infected with the lentiviral vectors for the delivery of OCT4, SOX2, and NANOG. Afterwards, the generated iPSCs were identified by morphology, pluripotency markers, global gene expression profiles, and epigenetic status both in vitro and in vivo. The results showed that we were able to reprogram hADCs by the defined factors (OCT4/SOX2/NANOG). The efficiency was significantly high (about 0.1%), and the typical colonies appeared on the 9th day after infection. They were similar to human embryonic stem (ES) cells in morphology, proliferation, surface markers, gene expression, and the epigenetic status of pluripotent cell-specific genes. Furthermore, these cells were able to differentiate into various cell types of all three germ layers both in vitro and in vivo. These results demonstrate that hADCs were an ideal somatic cell resource for the rapid and efficient generation of iPS cells by OCT4/SOX2/NANOG. (C) 2010 Published by Elsevier Ltd. on behalf of International Society of Differentiation

语种: 英语
项目资助者: scientific research foundation for the training of specialized talented person of Tangdu Hospital, FMMU, China
WOS记录号: WOS:000283602800008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64469
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.China Natl Ctr Int Res, Beijing 100191, Peoples R China
2.Fourth Mil Med Univ, Tangdu Hosp, Dept Gynecol & Obstet, Xian 710038, Peoples R China
3.Peking Univ, Stem Cell Res Ctr, Beijing 100191, Peoples R China
4.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Peoples R China
5.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Peoples R China
6.NW Polytech Univ, Fac Life Sci, Xian 710072, Peoples R China
7.Chinese Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China

Recommended Citation:
Zhao, Hong-xi,Li, Yang,Jin, Hai-feng,et al. Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG[J]. DIFFERENTIATION,2010,80(2-3):123-129.
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