IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their physical status in primary laryngeal squamous cell carcinoma
Liu, B.1; Lu, Z.2; Wang, P.1; Basang, Z.2; Rao, X.3
关键词human papillomavirus(HPV) laryngeal squamous cell carcinoma (LSCC)
刊名NEOPLASMA
2010
DOI10.4149/neo_2010_06_595
57期:6页:594-600
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CERVICAL-CARCINOMA ; VIRAL INTEGRATION ; NECK CANCERS ; IN-SITU ; HEAD ; DNA ; OROPHARYNX ; CARCINOGENESIS ; IDENTIFICATION ; EXPRESSION
英文摘要

Recently oncogenetic human papilloma virus(HPV) infection has been suggested to promote laryngeal squamous cell carcinoma(LSCC). To determine the prevalence and genotypes of HPV infection in laryngeal cancer specimens, 84 specimens from pathologically confirmed LSCC patients were studied for the presence of viral DNA and possible virus integration into the cellular genome. HPV genotyping was assayed prior to the integration analysis by using two PCR-based assays, including HPV-16 and-18 E7 type-specific and L1 general primers (GP5+/GP6+). Additionally, a quantitative real-time PCR (qRT-PCR) was used to examine the physical status of HPV-16 or-18 in HPV positive LSCC. Using HPV L1 general primer amplification, HPV DNA was detected in 23(27.4%) of the 84 LSCC samples. When PCR products were cloned and sequenced, HPV16 were found in all 23 L1 positive samples. However, when specific primers for HPV- 16 or -18 were used to amplify E6 and E7 in all samples, 29 cases (34.5%) were positive for HPV-16, while 6 cases (7.1%) were positive for HPV 18. Coinfection of HPV-16 and -18 were found in 4 cases (4.8%). In addition, qRT-PCR assay found that HPV-16 was characterized as episomal in 51.7% of cases, mixed (i.e., episomal and integrated) in 34.5%, and integrated in 13.8%, while HPV-18 was similarly characterized as episomal in 83.3% of cases and mixed in 16.7%. In the present study, about 36.9% of patients with LSCC were found to be infected with HPV-16 and -18 and integration of HPV-16 and -18 DNA into the host genome was found.

语种英语
WOS记录号WOS:000286494400014
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64509
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Head & Neck Surg,Minist Educ,Key Lab Carciog, Beijing 100142, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Genet,Minist Educ,Key Lab Carcinogenesis & T, Beijing 100142, Peoples R China
3.Peking Univ, Shougang Hosp, Dept Pathol, Beijing 100144, Peoples R China
推荐引用方式
GB/T 7714
Liu, B.,Lu, Z.,Wang, P.,et al. Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their physical status in primary laryngeal squamous cell carcinoma[J]. NEOPLASMA,2010,57(6):594-600.
APA Liu, B.,Lu, Z.,Wang, P.,Basang, Z.,&Rao, X..(2010).Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their physical status in primary laryngeal squamous cell carcinoma.NEOPLASMA,57(6),594-600.
MLA Liu, B.,et al."Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their physical status in primary laryngeal squamous cell carcinoma".NEOPLASMA 57.6(2010):594-600.
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