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学科主题: 药学
题名:
Identification of the absorptive constituents and their metabolites in vivo of Puerariae Lobatae Radix decoction orally administered in WZS-miniature pigs by HPLC-ESI-Q-TOFMS
作者: Zhou, Qi-Le1,2; Wang, Ying-Feng2; Yang, Dong-Hui1; Xu, Feng1; Zhao, Xin1; Zhang, Lei1; Liang, Jing1; Yang, Xiu-Wei1
关键词: Puerariae Lobatae Radix ; absorptive constituents ; metabolites ; WZS-miniature pig ; HPLC-ESI-Q-TOFMS
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2013-09-01
DOI: 10.1002/bmc.2928
卷: 27, 期:9, 页:1208-1218
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ; DANGGUI-BUXUE-TANG ; INTESTINAL BACTERIA ; GENISTEIN ; URINE ; RAT ; ELUCIDATION ; ISOFLAVONES ; DAIDZEIN ; MODEL
英文摘要:

In this study, the technique of high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-Q-TOFMS) was used to analyze and identify the absorptive constituents and their metabolites in drug-containing urine of Wuzhishan (WZS)-miniature pigs administered with Puerariae Lobatae Radix (PLR) decoction. With the accuratemassmeasurements (<5 ppm) and effective MS2 fragment ions, 96 compounds, including eight original constituents and 88metabolites, were identified from the drug-containing urine. Among these, 64 metabolites were new ones and their structures can be categorized into five types: isoflavones, puerols, O-desmethylangolensins, equols and isoflavanones. In particular, puerol-type constituents in PLR were first proved to be absorptive in vivo. Meanwhile, the metabolic pathways of PLR in vivo were investigated. On the basis of relative content of the identified compounds, 13 major metabolites accounting for approximately 50% of the contents, as well as their corresponding 12 prototype compounds, were determined as the major original absorptive constituents and metabolites of PLR in vivo. The HPLC-ESI-Q-TOFMS technique proved to be powerful for characterizing the chemical constituents from the complicated traditional Chinese medicine matrices in this research. Copyright (C) 2013 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2009ZX09502-006 ; 2009ZX09301-010-06
项目资助者: &prime ; Major New Medicine Project&prime ; in Mega-projects of Science Research of China ; National Science and Technology Major Project for Major New Drugs Innovation and Development of China
WOS记录号: WOS:000322717100018
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64510
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

Recommended Citation:
Zhou, Qi-Le,Wang, Ying-Feng,Yang, Dong-Hui,et al. Identification of the absorptive constituents and their metabolites in vivo of Puerariae Lobatae Radix decoction orally administered in WZS-miniature pigs by HPLC-ESI-Q-TOFMS[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(9):1208-1218.
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