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Effect of all-trans-retinoic acid on the expression of primordial germ cell differentiation-associated genes in mESC-derived EBs
Guo, Xin; Qi, Zheng-Yu; Zhang, Yan-Min; Qin, Jie; Cui, Guang-Hui; Gui, Yao-Ting; Cai, Zhi-Ming
关键词all-trans-retinoic acid embryoid body mouse embryonic stem cell primordial germ cell retinoic acid
刊名CELL BIOLOGY INTERNATIONAL
2012-05-01
DOI10.1042/CBI20110423
36期:5页:491-495
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]EMBRYONIC STEM-CELLS ; IN-VITRO ; MOUSE ; LIN28 ; MICE
英文摘要

atRA (all-trans-retinoic acid) is known to induce the differentiation of mESCs (mouse embryonic stem cells) into PGCs (primordial germ cells) in vitro. However, it is not clear as to what changes occur in PGC differentiation-associated genes or what mechanisms are involved when EBs (embryoid bodies) derived from mESCs are induced by atRA. EBs derived from mESCs were treated with 1, 2 or 5 mu M atRA for 16 h, 2 days or 5 days. Real-time PCR and Western blot analysis were performed to detect the relative levels of PGC differentiation-associated genes (Lin28, Blimp 1, Stra8 and Mvh) and the corresponding proteins respectively. Immunofluorescence was used to detect the protein location and distribution in EBs. The expression characteristics of genes could be divided into three categories: rapidly reached the peak value in 16 h and then decreased (Stra8, Lin28), initially low and then increased to reach the peak value in 5 days (Mvh) and relatively unchanged (Blimp1). A low level of Lin28 was expressed in EBs treated with atRA for 2 days or 5 days. The variation in the level of Lin28 mRNA did not influence the change in the level of Blimp1 mRNA. The changes in Stra8/Lin28 were consistent with the corresponding changes in the levels of their respective mRNAs, but the changes for Mvh/Blimp1 were not consistent with the corresponding changes in the levels of their respective mRNAs. Blimp1 expression may be independent of the effect of atRA on PGC differentiation. atRA may promote the start of a period in which there is a low level of Lin28 expression during PGC differentiation.

语种英语
WOS记录号WOS:000303550700011
项目编号30500543 ; 30700824 ; 30972992 ; 81100471 ; B2009230 ; 20090001120130 ; JC201005260214A ; 201001014
资助机构National Natural Science Foundation of China ; Medical Research Fund of Guangdong Province ; Ministry of Education of China ; Shenzhen Municipal R &amp ; D funding for basic research programs funded project ; Science and Technology Plan Key Projects of Shenzhen
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64516
专题北京大学深圳医院
作者单位Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China
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GB/T 7714
Guo, Xin,Qi, Zheng-Yu,Zhang, Yan-Min,et al. Effect of all-trans-retinoic acid on the expression of primordial germ cell differentiation-associated genes in mESC-derived EBs[J]. CELL BIOLOGY INTERNATIONAL,2012,36(5):491-495.
APA Guo, Xin.,Qi, Zheng-Yu.,Zhang, Yan-Min.,Qin, Jie.,Cui, Guang-Hui.,...&Cai, Zhi-Ming.(2012).Effect of all-trans-retinoic acid on the expression of primordial germ cell differentiation-associated genes in mESC-derived EBs.CELL BIOLOGY INTERNATIONAL,36(5),491-495.
MLA Guo, Xin,et al."Effect of all-trans-retinoic acid on the expression of primordial germ cell differentiation-associated genes in mESC-derived EBs".CELL BIOLOGY INTERNATIONAL 36.5(2012):491-495.
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