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学科主题: 临床医学
题名:
Effect of all-trans-retinoic acid on the expression of primordial germ cell differentiation-associated genes in mESC-derived EBs
作者: Guo, Xin; Qi, Zheng-Yu; Zhang, Yan-Min; Qin, Jie; Cui, Guang-Hui; Gui, Yao-Ting; Cai, Zhi-Ming
关键词: all-trans-retinoic acid ; embryoid body ; mouse embryonic stem cell ; primordial germ cell ; retinoic acid
刊名: CELL BIOLOGY INTERNATIONAL
发表日期: 2012-05-01
DOI: 10.1042/CBI20110423
卷: 36, 期:5, 页:491-495
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: EMBRYONIC STEM-CELLS ; IN-VITRO ; MOUSE ; LIN28 ; MICE
英文摘要:

atRA (all-trans-retinoic acid) is known to induce the differentiation of mESCs (mouse embryonic stem cells) into PGCs (primordial germ cells) in vitro. However, it is not clear as to what changes occur in PGC differentiation-associated genes or what mechanisms are involved when EBs (embryoid bodies) derived from mESCs are induced by atRA. EBs derived from mESCs were treated with 1, 2 or 5 mu M atRA for 16 h, 2 days or 5 days. Real-time PCR and Western blot analysis were performed to detect the relative levels of PGC differentiation-associated genes (Lin28, Blimp 1, Stra8 and Mvh) and the corresponding proteins respectively. Immunofluorescence was used to detect the protein location and distribution in EBs. The expression characteristics of genes could be divided into three categories: rapidly reached the peak value in 16 h and then decreased (Stra8, Lin28), initially low and then increased to reach the peak value in 5 days (Mvh) and relatively unchanged (Blimp1). A low level of Lin28 was expressed in EBs treated with atRA for 2 days or 5 days. The variation in the level of Lin28 mRNA did not influence the change in the level of Blimp1 mRNA. The changes in Stra8/Lin28 were consistent with the corresponding changes in the levels of their respective mRNAs, but the changes for Mvh/Blimp1 were not consistent with the corresponding changes in the levels of their respective mRNAs. Blimp1 expression may be independent of the effect of atRA on PGC differentiation. atRA may promote the start of a period in which there is a low level of Lin28 expression during PGC differentiation.

语种: 英语
所属项目编号: 30500543 ; 30700824 ; 30972992 ; 81100471 ; B2009230 ; 20090001120130 ; JC201005260214A ; 201001014
项目资助者: National Natural Science Foundation of China ; Medical Research Fund of Guangdong Province ; Ministry of Education of China ; Shenzhen Municipal R &amp ; D funding for basic research programs funded project ; Science and Technology Plan Key Projects of Shenzhen
WOS记录号: WOS:000303550700011
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64516
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China

Recommended Citation:
Guo, Xin,Qi, Zheng-Yu,Zhang, Yan-Min,et al. Effect of all-trans-retinoic acid on the expression of primordial germ cell differentiation-associated genes in mESC-derived EBs[J]. CELL BIOLOGY INTERNATIONAL,2012,36(5):491-495.
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